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<body> | <body> | ||
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+ | <div id="loader-bg"> | ||
+ | <div id="loader"> | ||
+ | <div class="photo-show"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png" width="600"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png" width="600"> | ||
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<!-- Sidebar/menu --> | <!-- Sidebar/menu --> | ||
<nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br> | <nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br> | ||
<a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a> | <a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a> | ||
− | <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px"> | + | <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px" id="wrap"> |
<img src="https://static.igem.org/mediawiki/2017/a/a8/T--TokyoTech--logo_white_bright_10211603.png" style="width: 100%"> | <img src="https://static.igem.org/mediawiki/2017/a/a8/T--TokyoTech--logo_white_bright_10211603.png" style="width: 100%"> | ||
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− | <div style="padding-top: 15px; padding-left: 25px;"> | + | <div style="padding-top: 15px; padding-left: 25px; id="wrap"> |
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+ | <li> | ||
<input type="checkbox" id="vmcb-d1" /> | <input type="checkbox" id="vmcb-d1" /> | ||
− | + | <label for="vmcb-d1"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Overview" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">Overview</a></label> | |
+ | </li> | ||
+ | |||
+ | <li style="padding-bottom: 10px; padding-top: 5px"> | ||
+ | <input type="checkbox" id="vmcb-d2" /> | ||
+ | <label for="vmcb-d2"><a style="text-align: center;">Bacteria to <br>Human Cells ▼</a></label> | ||
<ul> | <ul> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li> | ||
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Improvement <br>Assay</a></li> |
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br> Assay</a></li> | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br>Assay</a></li> |
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br> Analysis</a></li> | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br>Analysis</a></li> |
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br> Factor Assay</a></li> | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br>Factor Assay</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li style="padding-bottom: | + | <li style="padding-bottom: 3px"> |
− | <input type="checkbox" id="vmcb- | + | <input type="checkbox" id="vmcb-d3" /> |
− | <label for="vmcb- | + | <label for="vmcb-d3"><a style="text-align: center;">Human Cells to <br>Bacteria ▼</a></label> |
<ul> | <ul> | ||
− | <li | + | <li><a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">AHK4 Assay</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li | + | <li> |
− | <input type="checkbox" id="vmcb- | + | <input type="checkbox" id="vmcb-d4" /> |
− | <label for="vmcb- | + | <label for="vmcb-d4"><a href="https://2017.igem.org/Team:TokyoTech/InterLab" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">InterLab</a></label> |
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<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li> | ||
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Integrated | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Gold (Integrated)</a></li> |
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li> | ||
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<div class="w3-xxxlarge" style="padding-bottom: 3px;text-align: center"> | <div class="w3-xxxlarge" style="padding-bottom: 3px;text-align: center"> | ||
<figure> | <figure> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/e/e6/T--TokyoTech--bacteriatohuman.png" style="max-width:80%"> |
− | <figcaption style="font-size: 16px">Fig. Mechanism of signal transmission system from bacteria to human</figcaption> | + | <figcaption style="font-size: 16px">Fig. 1 Mechanism of signal transmission system from bacteria to human</figcaption> |
</figure> | </figure> | ||
</div> | </div> | ||
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<a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay"> | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay"> | ||
− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/2/23/Fig3_construction_of_TraI_gene3.png" style="width:100%"> |
<div class="w3-container"> | <div class="w3-container"> | ||
<h3>TraI Assay</h3> | <h3>TraI Assay</h3> | ||
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− | <a href=""> | + | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor"> |
− | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2017/1/15/T--TokyoTech--human_cell_circuit.png" style="width:100%"> |
<div class="w3-container"> | <div class="w3-container"> | ||
− | <h3> | + | <h3>Chimeric <br> Transcription Factor <br>Assay</h3> |
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+ | </a> | ||
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</div> | </div> | ||
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<a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay"> | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay"> | ||
− | <img src=" | + | <img src="https://static.igem.org/mediawiki/2017/1/14/T--TokyoTech--TraRAssay.png" style="width:100%"> |
<div class="w3-container"> | <div class="w3-container"> | ||
<h3>TraR Reporter <br> Assay</h3> | <h3>TraR Reporter <br> Assay</h3> | ||
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<div class="w3-light-grey"> | <div class="w3-light-grey"> | ||
<a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis"> | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis"> | ||
− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/1/10/T--TokyoTech--Transcriptome_malg2.png" style="width:100%"> |
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− | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/ | + | <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement"> |
− | <img src=" | + | <img src="https://static.igem.org/mediawiki/2017/d/db/T--TokyoTech--TraIimprove_11021228.png" style="width:100%"> |
<div class="w3-container"> | <div class="w3-container"> | ||
− | <h3> | + | <h3>TraI Improvement <br>Assay</h3> |
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<figure> | <figure> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/5/5a/T--TokyoTech--humantobacteria.png" style="width:80%"> |
− | <figcaption style="font-size: 16px">Fig. Mechanism of signal transmission system from human cells to bacteria</figcaption> | + | <figcaption style="font-size: 16px">Fig. 2 Mechanism of signal transmission system from human cells to bacteria</figcaption> |
</figure> | </figure> | ||
</div> | </div> | ||
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− | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/archive/5/54/20171030133245%21T--Tokyotech--AHK4construct.png" style="width:100%"> |
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<h3>AHK4 Assay</h3> | <h3>AHK4 Assay</h3> | ||
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<h4 style="text-align: center">InterLab</h4> | <h4 style="text-align: center">InterLab</h4> | ||
− | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px"> | + | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px;padding-bottom:20px"> |
In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015). | In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015). | ||
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+ | <div class="w3-container" id="contact" style="margin-top:20px"> | ||
+ | <p id="pageTop" style="text-align:right"><a href="#wrap"><img src="https://static.igem.org/mediawiki/2017/0/0d/T--TokyoTech--page_top_2.png" style="width:200px"></a></p> | ||
</div> | </div> | ||
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− | <div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"> | + | <div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"><a href="http://96haji.me/" title="W3.CSS" target="_blank" class="w3-hover-opacity">Hajime Fujita with W3.CSS: All Rights Reserved</a></p></div> |
+ | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script> | ||
<script> | <script> | ||
// Script to open and close sidebar | // Script to open and close sidebar | ||
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document.getElementById("myOverlay").style.display = "none"; | document.getElementById("myOverlay").style.display = "none"; | ||
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} | } | ||
+ | $(function() { | ||
+ | var h = $(window).height(); | ||
+ | |||
+ | $('#wrap').css('display','none'); | ||
+ | $('#loader-bg ,#loader').height(h).css('display','block'); | ||
+ | }); | ||
+ | |||
+ | $(window).load(function () { //全ての読み込みが完了したら実行 | ||
+ | $('#loader-bg').delay(900).fadeOut(800); | ||
+ | $('#loader').delay(600).fadeOut(300); | ||
+ | $('#wrap').css('display', 'block'); | ||
+ | }); | ||
+ | </script> | ||
+ | <script> | ||
+ | //■page topボタン | ||
+ | $(function(){ | ||
+ | var topBtn=$('#pageTop'); | ||
+ | topBtn.hide(); | ||
+ | |||
+ | //◇ボタンの表示設定 | ||
+ | $(window).scroll(function(){ | ||
+ | if($(this).scrollTop()>80){ | ||
+ | //---- 画面を80pxスクロールしたら、ボタンを表示する | ||
+ | topBtn.fadeIn(); | ||
+ | }else{ | ||
+ | //---- 画面が80pxより上なら、ボタンを表示しない | ||
+ | topBtn.fadeOut(); | ||
+ | } | ||
+ | }); | ||
+ | |||
+ | // ◇ボタンをクリックしたら、スクロールして上に戻る | ||
+ | topBtn.click(function(){ | ||
+ | $('body,html').animate({ | ||
+ | scrollTop: 0},500); | ||
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+ | }); | ||
</script> | </script> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 03:40, 2 November 2017
<!DOCTYPE html>
![](https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png)
![](https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png)
Experiment: Overview
Introduction
For convenience of description, we devided experimental section into two parts. One is a series of assays to validate signal transmission system from bacteria to human cells. The other is a assay (AHK4 Assay) to validate the reverse system from human cells to bacteria. You can see the list of assays we conducted and also, a summary of InterLab activity.
Signal transmission system from bacteria to human cells
~ Integration of systems derived from bacteria and human cells ~
In our system, the transcription level is controlled by integrating quorum sensing (bacterial cell-to-cell communication) and NF-kB, transcription factor in mammalian cell. We used this system the signal transmission from bacteria to human cells. To validate the system, we conducted the assays shown below.
![](https://static.igem.org/mediawiki/2017/e/e6/T--TokyoTech--bacteriatohuman.png)
Signal transmission system from human cells to bacteria
~ Integration of systems derived from bacteria and plant cells ~
In our system, the transcription level is controlled by integrating signal transmission systems derived from bacteria and plants. We used this system the signal transmission from human cells to bacteria.
![](https://static.igem.org/mediawiki/2017/5/5a/T--TokyoTech--humantobacteria.png)
InterLab
In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015).