Difference between revisions of "Team:IISc-Bangalore"

 
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          <div class="gifAnimated" style="background-image: url('https://static.igem.org/mediawiki/2017/4/43/T--IISc-Bangalore--iFLOAT-final.gif');"></div>
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<h1> iFLOAT: Isolation of Floater-Linked Overexpressed-protein Ascender Technology </h1>
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<p>iFLOAT is our attempt to develop a novel protein purification strategy using cyanobacterial gas vesicle tagged proteins. Our project strives to reduce downstream recombinant protein purification costs and will vastly improve cost-effectiveness and productivity of industries reliant on recombinant protein production.
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Our aim is to reduce the cost of downstream processing and purification of recombinant proteins. To facilitate this, we are engineering E. coli to express gas vesicle proteins (gvpA and gvpC) from cyanobacteria (Planktothrix rubescens). The recombinant protein of interest will be fused to gvpC using an amino acid linker comprising the sequence specific to the enzyme TEV protease (EDLYFQ|S).
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When the cells have overexpressed enough of the recombinant protein, we will lyse the cells and obtain a protein extract. Since the recombinant protein of interest is fused to a gas vesicle, it will float to the surface of the protein extract and can be skimmed off. After the desired level of purity is obtained (by repeated resuspension and skimming), TEV protease can be added to cleave the recombinant protein of interest from the gas vesicle, dispersing it into the solution while the pure gas vesicles remain at the surface.
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          <div style="position: absolute; right: 5%; width: 35%; font-size: 2.3em; line-height: 1.4; font-family: 'Poppins'; top: 50%; transform: translateY(-50%);">Gas vesicles (GVs) are hollow protein nanostructures synthesized by phototrophic haloarchaea and cyanobacteria to regulate their flotation in aquatic habitats. </div>
  
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For our project, we will demonstrate a proof of concept using the recombinant protein sfGFP, which we will tag to gvpC. Following this, we will demonstrate the same system in Pichia pastoris to show that eukaryotic protein purification can also be simplified by our method.</p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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          <div class="gifAnimated" style="background-image: url('https://static.igem.org/mediawiki/2017/6/63/T--IISc-Bangalore--home-2.gif'); background-position: right;"></div>
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          <div style="position: absolute; left: 3%; width: 33%; font-size: 2.3em; line-height: 1.4; font-family: 'Poppins', sans-serif; top: 55%; transform: translateY(-50%);">Bioengineered GVs have been genetically modified for diverse purposes; ultrasonic molecular imaging, gauging cellular turgor pressures, and vaccine delivery - but none of their current applications exploit their most fundamental characteristic: buoyancy.</div>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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          <div class="gifAnimated" style="background-image: url('https://static.igem.org/mediawiki/2017/4/45/T--IISc-Bangalore--home-3.gif'); background-position: left;"></div>
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          <div style="position: absolute; right: 5%; width: 20%; font-size: 2.3em; line-height: 1.4; font-family: 'Poppins'; top: 55%; transform: translateY(-50%);">Our modelling shows that clusters of GVs float enormously better than individual GVs.</div>
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          <div class="gifAnimated" style="background-image: url('https://static.igem.org/mediawiki/2017/7/71/T--IISc-Bangalore--home-4.gif'); background-position: right;"></div>
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          <div style="position: absolute; left: 3%; width: 30%; font-size: 2.3em; line-height: 1.4; font-family: 'Poppins', sans-serif; top: 55%; transform: translateY(-50%);">iFLOAT aims to improve the flotation of gas vesicles by clustering them using three distinct methods: charge-based flocculation, biotin-streptavidin interaction, and SpyCatcher-SpyTag heterodimerization</div>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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          <div style="position: absolute; right: 5%; width: 25%; font-size: 2.3em; line-height: 1.4; font-family: 'Poppins'; top: 55%; transform: translateY(-50%);"> Future applications of these clusters can include bioremediation of oil spills, flotation-based separation, and purification of specific targets from mixtures!</div>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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              <a href="https://2017.igem.org/Team:IISc-Bangalore/Team"><img src='https://static.igem.org/mediawiki/2017/8/8e/T--IISc-Bangalore--icons-about.svg' width=100% />
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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              <div style="font-size: 2.5em; font-family: 'Raleway-ExtraBold', sans-serif; margin-top: 20px; text-align: center; color: black;" class='textclass'>ABOUT</div></a>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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              <a href="https://2017.igem.org/Team:IISc-Bangalore/Description"><img src='https://static.igem.org/mediawiki/2017/3/3b/T--IISc-Bangalore--icons-project.svg' width=100% />
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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              <div style="font-size: 2.5em; font-family: 'Raleway-ExtraBold', sans-serif; margin-top: 20px; text-align: center; color: black;" class='textclass'>PROJECT</div></a>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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              <a href="https://2017.igem.org/Team:IISc-Bangalore/Assembly"><img src='https://static.igem.org/mediawiki/2017/6/6f/T--IISc-Bangalore--icons-lab.svg' width=100% />
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<li>Have lots of fun! </li>
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              <div style="font-size: 2.5em; font-family: 'Raleway-ExtraBold', sans-serif; margin-top: 20px; text-align: center; color: black;" class='textclass'>LAB</div></a>
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              <a href="https://2017.igem.org/Team:IISc-Bangalore/Hardware"><img src='https://static.igem.org/mediawiki/2017/f/f7/T--IISc-Bangalore--icons-hardware.svg' width=100% />
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              <div style="font-size: 2.5em; font-family: 'Raleway-ExtraBold', sans-serif; margin-top: 20px; text-align: center; color: black;" class='textclass'>HARDWARE</div></a>
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              <a href="https://2017.igem.org/Team:IISc-Bangalore/HP/Silver"><img src='https://static.igem.org/mediawiki/2017/8/8f/T--IISc-Bangalore--icons-community.svg' width=100% />
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              <div style="font-size: 2.5em; font-family: 'Raleway-ExtraBold', sans-serif; margin-top: 20px; text-align: center; color: black;" class='textclass'>COMMUNITY</div></a>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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    anchors:['first', 'second', 'third', 'fourth', 'fifth', 'sixth'],
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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Latest revision as of 03:41, 2 November 2017

Gas vesicles (GVs) are hollow protein nanostructures synthesized by phototrophic haloarchaea and cyanobacteria to regulate their flotation in aquatic habitats.
Bioengineered GVs have been genetically modified for diverse purposes; ultrasonic molecular imaging, gauging cellular turgor pressures, and vaccine delivery - but none of their current applications exploit their most fundamental characteristic: buoyancy.
Our modelling shows that clusters of GVs float enormously better than individual GVs.
iFLOAT aims to improve the flotation of gas vesicles by clustering them using three distinct methods: charge-based flocculation, biotin-streptavidin interaction, and SpyCatcher-SpyTag heterodimerization
Future applications of these clusters can include bioremediation of oil spills, flotation-based separation, and purification of specific targets from mixtures!