Difference between revisions of "Team:MSU-Michigan/HP/Gold Integrated"

 
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<h3>★  ALERT! </h3>
 
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1>Human Practices</h1>
 
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<h3><b>East Lansing Water Treatment Facility</b></h3>
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We visited the plant in hopes of leaving with a better idea on how to tackle the current issue of contaminated water supply.  Bob, the manager of the plant, took us on a full tour displaying everything he could from where the water entered to where the solids and the clean water departed.  Bob spoke told us about how water treatment is expensive and that the plant is barely getting by and using 40 year old equipment and machines.  Late in the tour he told us some stories from when he visited some small villages in Nigeria where the people would drink water that was clearly contaminated as they had no choice and little knowledge of the contamination.  This encouraged us to pursue the idea of creating a cheap water contaminant detector.
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<img src="https://static.igem.org/mediawiki/2017/thumb/2/22/MSUteamphoto.jpeg/1200px-MSUteamphoto.jpeg" height="" width="">
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<img src="https://static.igem.org/mediawiki/2017/thumb/b/b3/MSU-Michigan_WWTL1.jpeg/1200px-MSU-Michigan_WWTL1.jpeg">
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<h3><b>Bioreactors as a safe measurement device and educational tool</b></h3>
  
<h1>Gold Medal and Integrated Human Practices</h1>
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<p>MSU-iGEM 2017 drew inspiration for our project from the Flint Michigan water crisis. Fresh water contamination continues to impact wildlife and humans throughout the globe with contaminants ranging from oil to heavy metals and pesticides/pharmaceuticals. Contaminants such as lead are regulated by the EPA but pesticides, heavy metals, and pharmaceuticals go unregulated. We wanted to emphasize detecting contaminants that are not regulated in hopes of developing a system that can even be incorporated into water treatment facilities. We visited the East Lansing Water Treatment Plant to gather information on how to apply our project. We continued to develop the impact of our project by participating in an even with the Society of Women Engineers. We wanted to show high schoolers the possibilities of synthetic biology and test to see if bioreactors could be used as a teaching tool. Finally, we sent our bioreactors to the Purdue University iGEM team to test reproducibility of our results. We also wanted to test if our system could be used based on a detailed protocol posted on University of Michigan Software’s website ProtoCat.</p>
  
<p>This page will contain information for your Gold medal Human Practices work, which you can also use to nominate your team for the Best Integrated Human Practices page. To make things easier, we have combined the Gold medal page with the Best Integrated Human Practices page since we expect the work to overlap considerably. </p>
 
<p>iGEM teams are unique and leading the field because they "go beyond the lab" to imagine their projects in a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
 
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
 
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
 
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<a href="https://protocat.org/user/68/">View MSU iGEM protocol on ProtoCat</a>
  
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<h3>Gold Medal Criterion #1</h3>
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<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.</p>
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<h1>Paper Microbial Fuel Cells</h1>
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<img src="https://static.igem.org/mediawiki/2017/8/8e/MSU-Michigan_paper_mfc.png">
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<img src="https://static.igem.org/mediawiki/2017/6/63/MSU-Michigan_papermfc.jpeg">
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<p>Following our work with the bioreactors, we wished to pursue an even simpler and more inexpensive alternative to our design. Paper microbial fuel cells (MFCs) represent a new design to the traditional fuel cell (1). Their compact size and self sufficiency allow for a more user friendly mechanism for measuring current. We tested these (MFCs) extensively and found that there are two crucial keys to success for these cells. These two things include: optimal connections between adjoining MFCs and standardization of the size within each component of the MFC. We troubleshooted the first problem by hand-building an acrylic compression system to hold the MFCs in place. The MFCs are also readily disposable by flame, which will sterilize any biohazard, and are easy to use.  We unfortunately were unable to finish troubleshooting the second main problem but managed to obtain up to 1.8mA of current across six MFCs that maintained good connections. We would want to standardize the MFCs by 3D printing them which would allow for optimal connections and a successful system as displayed in the literature (1).</p>  
  
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<a href="https://2017.igem.org/Team:MSU-Michigan/Engagement" class="w3-button w3-xxlarge w3-padding-large w3-green w3-ripple w3-round w3-hover-white">More Engagement</a>
 
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<h3>Best Integrated Human Practices Special Prize</h3>
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<h1>Updated Biobrick 3A Assembly Protocol</h1>
  
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<a href="http://parts.igem.org/Help:Protocols/3A_Assembly" class="w3-button w3-xxlarge w3-padding-large w3-green w3-ripple w3-round w3-hover-white">iGEM Protocol</a>
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<h2>SUGGESTIONS FOR ALL iGEM TEAMS THAT NEED HELP</h2>
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<h3>Digestion</h3>
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<p>
To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Integrated Human Practices prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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<br>1. Digest for one hour
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<br>2. Heat kill at 80C for 20 min.
You must also delete the message box on the top of this page to be eligible for this prize.
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<h4>Ligation</h4>
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<h5>Inspiration</h5>
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<p>Here are a few examples of excellent Integrated Human Practices work:</p>
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<br>1. Use 2:1 molar ratio of insert to vector
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<br>2. Ligate overnight
<li><a href="https://2016.igem.org/Team:INSA-Lyon/Integrated_Practices">2016 INSA Lyon</a></li>
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<br>3. Heat kill at 80C for 20min.
<li><a href="https://2016.igem.org/Team:UofC_Calgary/Integrated_Practices">2016 UofC Calgary</a></li>
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<li><a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Practices">2015 Bielefeld</a></li>
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<li><a href="https://2015.igem.org/Team:Edinburgh/Practices">2015 Edinburgh</a></li>
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<h5>Transformation</h5>
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<br>1. Use high efficiency cells
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<br>2. Use a minimum of 5μL of ligation mixture
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<h1>References</h1>
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<br>(1) Lee, S. H.; Ban, J. Y.; Oh, C.-H.; Park, H.-K.; Choi, S. Sci. Rep. 2016, 6 (1), 28588.
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Latest revision as of 03:49, 2 November 2017

Human Practices

East Lansing Water Treatment Facility

We visited the plant in hopes of leaving with a better idea on how to tackle the current issue of contaminated water supply. Bob, the manager of the plant, took us on a full tour displaying everything he could from where the water entered to where the solids and the clean water departed. Bob spoke told us about how water treatment is expensive and that the plant is barely getting by and using 40 year old equipment and machines. Late in the tour he told us some stories from when he visited some small villages in Nigeria where the people would drink water that was clearly contaminated as they had no choice and little knowledge of the contamination. This encouraged us to pursue the idea of creating a cheap water contaminant detector.

Bioreactors as a safe measurement device and educational tool

MSU-iGEM 2017 drew inspiration for our project from the Flint Michigan water crisis. Fresh water contamination continues to impact wildlife and humans throughout the globe with contaminants ranging from oil to heavy metals and pesticides/pharmaceuticals. Contaminants such as lead are regulated by the EPA but pesticides, heavy metals, and pharmaceuticals go unregulated. We wanted to emphasize detecting contaminants that are not regulated in hopes of developing a system that can even be incorporated into water treatment facilities. We visited the East Lansing Water Treatment Plant to gather information on how to apply our project. We continued to develop the impact of our project by participating in an even with the Society of Women Engineers. We wanted to show high schoolers the possibilities of synthetic biology and test to see if bioreactors could be used as a teaching tool. Finally, we sent our bioreactors to the Purdue University iGEM team to test reproducibility of our results. We also wanted to test if our system could be used based on a detailed protocol posted on University of Michigan Software’s website ProtoCat.

View MSU iGEM protocol on ProtoCat


Paper Microbial Fuel Cells



Following our work with the bioreactors, we wished to pursue an even simpler and more inexpensive alternative to our design. Paper microbial fuel cells (MFCs) represent a new design to the traditional fuel cell (1). Their compact size and self sufficiency allow for a more user friendly mechanism for measuring current. We tested these (MFCs) extensively and found that there are two crucial keys to success for these cells. These two things include: optimal connections between adjoining MFCs and standardization of the size within each component of the MFC. We troubleshooted the first problem by hand-building an acrylic compression system to hold the MFCs in place. The MFCs are also readily disposable by flame, which will sterilize any biohazard, and are easy to use. We unfortunately were unable to finish troubleshooting the second main problem but managed to obtain up to 1.8mA of current across six MFCs that maintained good connections. We would want to standardize the MFCs by 3D printing them which would allow for optimal connections and a successful system as displayed in the literature (1).




Updated Biobrick 3A Assembly Protocol



SUGGESTIONS FOR ALL iGEM TEAMS THAT NEED HELP


Digestion



1. Digest for one hour
2. Heat kill at 80C for 20 min.


Ligation



1. Use 2:1 molar ratio of insert to vector
2. Ligate overnight
3. Heat kill at 80C for 20min.


Transformation


1. Use high efficiency cells
2. Use a minimum of 5μL of ligation mixture




References


(1) Lee, S. H.; Ban, J. Y.; Oh, C.-H.; Park, H.-K.; Choi, S. Sci. Rep. 2016, 6 (1), 28588.


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