Difference between revisions of "Team:SDSZ-China/Parts"

(Prototype team page)
 
 
(12 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<html>
 
<html>
  
 +
<head>
 +
    <title>Project</title>
  
 +
    <style type='text/css'>
 +
        #top_title, #sideMenu {
 +
            display: none;
 +
        }
  
 +
        #content {
 +
            width: 100%;
 +
            margin: 0;
 +
            padding: 0;
 +
            background: #f3f4f4;
 +
        }
  
 +
        p {
 +
            font-family: Lato !important;
 +
            font-size: 17px !important
 +
        }
  
 +
        h1 {
 +
            font-size: 40 -px !important;
 +
        }
  
−
<div class="column full_size">
+
        body {
 +
            font-family: Lato !important;
 +
        }
  
−
<h1>Parts</h1>
+
        h3 {
 +
            font-size: 25 -px;
 +
        }
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
        #bg {
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
            position: fixed;
 +
            z-index: -1;
 +
            top: 0;
 +
            left: 0;
 +
        }
 +
    </style>
 +
    <link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'>
 +
    <!-- 最新版本的 Bootstrap 核心 CSS 文件 -->
 +
    <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css"
 +
          integrity="sha384-BVYiiSIFeK1dGmJRAkycuHAHRg32OmUcww7on3RYdg4Va+PmSTsz/K68vbdEjh4u" crossorigin="anonymous">
  
  
−
</div>
+
    <script>localStorage.clear();</script>
 +
    <!-- 最新的 Bootstrap 核心 JavaScript 文件 -->
 +
    <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"
 +
            integrity="sha384-Tc5IQib027qvyjSMfHjOMaLkfuWVxZxUPnCJA7l2mCWNIpG9mGCD8wGNIcPD7Txa"
 +
            crossorigin="anonymous"></script>
  
 +
    <script type="text/javascript" async
 +
            src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script>
 +
    <script type="text/x-mathjax-config">
 +
        MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}});
  
  
  
 +
   
 +
   
 +
   
 +
    </script>
  
−
<div class="column half_size">
+
    <script type="text/javascript">
−
<div class="highlight">
+
−
<h5>Note</h5>
+
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
−
</div>
+
−
</div>
+
  
 +
        $(document).ready(function () {
 +
            $("#category_navbar a").on('click', function (event) {
 +
                // Make sure this.hash has a value before overriding default behavior
 +
                if (this.hash !== "") {
 +
                    // Prevent default anchor click behavior
 +
                    event.preventDefault();
  
 +
                    // Store hash0
 +
                    var hash = this.hash;
  
 +
                    // Using jQuery's animate() method to add smooth page scroll
 +
                    // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
 +
                    $('html, body').animate({
 +
                        scrollTop: $(hash).offset().top
 +
                    }, 800, function () {
  
−
<div class="column half_size">
+
                        // Add hash (#) to URL when done scrolling (default click behavior)
 +
                        window.location.hash = hash;
 +
                    });
 +
                }  // End if
 +
            });
 +
        });
  
−
<h5>Adding parts to the registry</h5>
 
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
−
</div>
 
  
 +
    </script>
  
 +
</head>
 +
</head>
  
 +
<body data-spy="scroll" data-target="#category_navbar">
  
 +
<div class="container" style='z-index:10;position:relative;'>
  
−
<div class="column half_size">
 
  
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
    <div class="row card" id='bodycontainer' class='row'>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
        <nav class="navbar navbar-default navbar-static-top">
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
            <!-- Brand and toggle get grouped for better mobile display -->
 +
            <div class="navbar-header">
 +
                <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 +
                        data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
 +
                    <span class="sr-only">Toggle navigation</span>
 +
                    <span class="icon-bar"></span>
 +
                    <span class="icon-bar"></span>
 +
                    <span class="icon-bar"></span>
 +
                </button>
 +
                <a class="navbar-brand" href="#"><img
 +
                        src="https://static.igem.org/mediawiki/2017/8/8c/%E5%91%80%E5%95%8A%E5%93%88.jpeg" width="100"></a>
 +
            </div>
  
−
</div>
+
            <!-- Collect the nav links, forms, and other content for toggling -->
 +
            <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 +
                <ul class="nav navbar-nav">
 +
                    <li><a href="/Team:SDSZ-China">Home</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Project">Project</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Hardware">Hardware</a></li>
 +
                </ul>
 +
                <ul class="nav navbar-nav navbar-right">
 +
                    <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li>
 +
                    <li><a href="#">By SDSZ</a></li>
 +
                </ul>
 +
            </div><!-- /.navbar-collapse -->
 +
        </nav>
  
  
−
<div class="column half_size">
+
        <div class="col-sm-2" style='padding-left:5% padding-right:10%'>
 +
            <div id="category_navbar">
 +
                <ul class="nav nav-list" data-spy="affix" data-offset-top="160"
 +
                    style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
 +
                    <li><a href="#Parts">Parts</a></li>
 +
                    <li><a href="#Contribution">Contribution</a></li>
 +
                </ul>
 +
            </div>
  
−
<h5>Inspiration</h5>
+
        </div>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
        <div class="col-sm-10" style="padding-right:5%">
−
<ul>
+
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column full_size">
+
            <h1 id="Parts">Parts</h1>
−
<h5>Part Table </h5>
+
            <div>
 +
                <table>
 +
                    <thead>
 +
                    <tr>
 +
                        <th>Name</th>
 +
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designer</th>
 +
                        <th>Length</th>
 +
                    </tr>
 +
                    </thead>
 +
                    <tbody>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239001'>BBa_K2239001</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1089</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239002'>BBa_K2239002</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1179</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239003'>BBa_K2239003</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1323</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239004'>BBa_K2239004</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1107</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239005'>BBa_K2239005</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1047</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239006'>BBa_K2239006</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1080</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239007'>BBa_K2239007</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1170</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239008'>BBa_K2239008</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1314</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239009'>BBa_K2239009</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1098</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239010'>BBa_K2239010</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1038</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011'>BBa_K2239011</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7alpha-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>780</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239012'>BBa_K2239012</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7beta-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>870</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239013'>BBa_K2239013</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>738</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239014'>BBa_K2239014</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GDH (glucose dehydrogenas)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>798</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239015'>BBa_K2239015</a></td>
 +
                        <td>Coding</td>
 +
                        <td>LDH ( lactate dehydrogenase)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1014</td>
 +
                    </tr>
 +
                    </tbody>
 +
                </table>
 +
            </div>
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
            <h1 id="Contribution">Contribution</h1>
 +
            <p>We improved the description of CBD and ChBD on previous part pages.</p>
 +
            <h2>Proof of the CBD function</h2>
 +
            <p>The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is
 +
                recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not
 +
                significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is
 +
                left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).</p>
 +
            <div class="row">
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'/>
 +
                    <p>[Fig. 1. GFP-CBD on gauze before washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'/>
 +
                    <p>[Fig. 2. GFP-CBD on gauze after washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'/>
 +
                    <p>[Fig. 3. GFP-ChBD on gauze before washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'/>
 +
                    <p>[Fig. 4. GFP-ChBD on gauze after washing]</p></div></figure>
 +
            </div>
 +
            <h2>Proof of the ChBD function</h2>
 +
            <p>The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but
 +
                without chitin, solution with the presence of GFP and chitin, and water in comparison, are
 +
                presented.</p>
 +
            <p>Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder
 +
                inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in
 +
                tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the
 +
                color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a
 +
                result, the ChBD is well functioned.</p>
 +
            <p></p>
 +
            <div class="row">
 +
                <figure class="col-sm-3"><div class="thumbnail">
 +
                    <img src='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'
 +
                        alt='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'/>
 +
                    <p>[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4,
 +
                        water]</p></div></figure>
 +
                <p></p>
  
−
<div class="highlight">
+
            </div>
  
  
−
</html>
+
            <br>
−
<groupparts>iGEM17 SDSZ-China</groupparts>
+
            <br>
 +
            <br>
 +
        </div>
 +
    </div>
  
−
<html>
+
    <div class="backgroundpic">
 +
        <img src="https://static.igem.org/mediawiki/2017/e/e3/%E8%83%8C%E6%99%AF2jpeg.jpeg" width="100%"
 +
            height="100%" id="bg" alt="">
 +
    </div>
 
</div>
 
</div>
−
</div>
 
−
 
−
 
  
 +
</body>
  
 
</html>
 
</html>

Latest revision as of 03:49, 2 November 2017


Project

Parts

Name Type Description Designer Length
BBa_K2239001 Generator ChBD-7alphaHSDH Ruohong Wang 1089
BBa_K2239002 Generator ChBD-7betaHSDH Ruohong Wang 1179
BBa_K2239003 Generator ChBD-LDH Ruohong Wang 1323
BBa_K2239004 Generator ChBD-GDH Ruohong Wang 1107
BBa_K2239005 Generator ChBD-GFP Ruohong Wang 1047
BBa_K2239006 Generator CBD-7alphaHSDH Ruohong Wang 1080
BBa_K2239007 Generator CBD-7betaHSDH Ruohong Wang 1170
BBa_K2239008 Generator CBD-LDH Ruohong Wang 1314
BBa_K2239009 Generator CBD-GDH Ruohong Wang 1098
BBa_K2239010 Generator CBD-GFP Ruohong Wang 1038
BBa_K2239011 Coding 7alpha-HSDH Ruohong Wang 780
BBa_K2239012 Coding 7beta-HSDH Ruohong Wang 870
BBa_K2239013 Coding GFP Ruohong Wang 738
BBa_K2239014 Coding GDH (glucose dehydrogenas) Ruohong Wang 798
BBa_K2239015 Coding LDH ( lactate dehydrogenase) Ruohong Wang 1014

Contribution

We improved the description of CBD and ChBD on previous part pages.

Proof of the CBD function

The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).

https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png

[Fig. 1. GFP-CBD on gauze before washing]

https://static.igem.org/mediawiki/2017/d/de/2_CBD.png

[Fig. 2. GFP-CBD on gauze after washing]

https://static.igem.org/mediawiki/2017/7/70/3_CBD.png

[Fig. 3. GFP-ChBD on gauze before washing]

https://static.igem.org/mediawiki/2017/1/13/4_CBD.png

[Fig. 4. GFP-ChBD on gauze after washing]

Proof of the ChBD function

The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but without chitin, solution with the presence of GFP and chitin, and water in comparison, are presented.

Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a result, the ChBD is well functioned.

https://static.igem.org/mediawiki/2017/0/07/5_CBD.png

[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4, water]