Difference between revisions of "Team:SDSZ-China/Parts"

 
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                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Hardware">Hardware</a></li>
 
                 </ul>
 
                 </ul>
 
                 <ul class="nav navbar-nav navbar-right">
 
                 <ul class="nav navbar-nav navbar-right">
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                     style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
 
                     style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
 
                     <li><a href="#Parts">Parts</a></li>
 
                     <li><a href="#Parts">Parts</a></li>
 +
                    <li><a href="#Contribution">Contribution</a></li>
 
                 </ul>
 
                 </ul>
 
             </div>
 
             </div>
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         <div class="col-sm-10" style="padding-right:5%">
 
         <div class="col-sm-10" style="padding-right:5%">
  
 +
            <h1 id="Parts">Parts</h1>
 
             <div>
 
             <div>
 
                 <table>
 
                 <table>
−
<thead>
+
                    <thead>
−
<tr><th>Name</th><th>Type</th><th>Description</th><th>Designer</th><th>Length</th></tr></thead>
+
                    <tr>
−
<tbody><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239001'>BBa_K2239001</a></td><td>Generator</td><td>ChBD-7alphaHSDH</td><td>Ruohong Wang</td><td>1089</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239002'>BBa_K2239002</a></td><td>Generator</td><td>ChBD-7betaHSDH</td><td>Ruohong Wang</td><td>1179</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239003'>BBa_K2239003</a></td><td>Generator</td><td>ChBD-LDH</td><td>Ruohong Wang</td><td>1323</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239004'>BBa_K2239004</a></td><td>Generator</td><td>ChBD-GDH</td><td>Ruohong Wang</td><td>1107</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239005'>BBa_K2239005</a></td><td>Generator</td><td>ChBD-GFP</td><td>Ruohong Wang</td><td>1047</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239006'>BBa_K2239006</a></td><td>Generator</td><td>CBD-7alphaHSDH</td><td>Ruohong Wang</td><td>1080</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239007'>BBa_K2239007</a></td><td>Generator</td><td>CBD-7betaHSDH</td><td>Ruohong Wang</td><td>1170</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239008'>BBa_K2239008</a></td><td>Generator</td><td>CBD-LDH</td><td>Ruohong Wang</td><td>1314</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239009'>BBa_K2239009</a></td><td>Generator</td><td>CBD-GDH</td><td>Ruohong Wang</td><td>1098</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239010'>BBa_K2239010</a></td><td>Generator</td><td>CBD-GFP</td><td>Ruohong Wang</td><td>1038</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011'>BBa_K2239011</a></td><td>Coding</td><td>7alpha-HSDH</td><td>Ruohong Wang</td><td>780</td></tr><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239012'>BBa_K2239012</a></td><td>Coding</td><td>7beta-HSDH</td><td>Ruohong Wang</td><td>870</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239013'>BBa_K2239013</a></td><td>Coding</td><td>GFP</td><td>Ruohong Wang</td><td>738</td></tr><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239014'>BBa_K2239014</a></td><td>Coding</td><td>GDH (glucose dehydrogenas)</td><td>Ruohong Wang</td><td>798</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239015'>BBa_K2239015</a></td><td>Coding</td><td>LDH ( lactate dehydrogenase)</td><td>Ruohong Wang</td><td>1014</td></tr></tbody>
+
                        <th>Name</th>
−
</table>
+
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designer</th>
 +
                        <th>Length</th>
 +
                    </tr>
 +
                    </thead>
 +
                    <tbody>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239001'>BBa_K2239001</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1089</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239002'>BBa_K2239002</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1179</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239003'>BBa_K2239003</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1323</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239004'>BBa_K2239004</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1107</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239005'>BBa_K2239005</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1047</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239006'>BBa_K2239006</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1080</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239007'>BBa_K2239007</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1170</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239008'>BBa_K2239008</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1314</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239009'>BBa_K2239009</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1098</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239010'>BBa_K2239010</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1038</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011'>BBa_K2239011</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7alpha-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>780</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239012'>BBa_K2239012</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7beta-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>870</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239013'>BBa_K2239013</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>738</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239014'>BBa_K2239014</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GDH (glucose dehydrogenas)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>798</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239015'>BBa_K2239015</a></td>
 +
                        <td>Coding</td>
 +
                        <td>LDH ( lactate dehydrogenase)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1014</td>
 +
                    </tr>
 +
                    </tbody>
 +
                </table>
 
             </div>
 
             </div>
 +
 +
            <h1 id="Contribution">Contribution</h1>
 +
            <p>We improved the description of CBD and ChBD on previous part pages.</p>
 +
            <h2>Proof of the CBD function</h2>
 +
            <p>The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is
 +
                recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not
 +
                significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is
 +
                left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).</p>
 +
            <div class="row">
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'/>
 +
                    <p>[Fig. 1. GFP-CBD on gauze before washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'/>
 +
                    <p>[Fig. 2. GFP-CBD on gauze after washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'/>
 +
                    <p>[Fig. 3. GFP-ChBD on gauze before washing]</p></div></figure>
 +
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'
 +
                                              alt='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'/>
 +
                    <p>[Fig. 4. GFP-ChBD on gauze after washing]</p></div></figure>
 +
            </div>
 +
            <h2>Proof of the ChBD function</h2>
 +
            <p>The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but
 +
                without chitin, solution with the presence of GFP and chitin, and water in comparison, are
 +
                presented.</p>
 +
            <p>Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder
 +
                inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in
 +
                tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the
 +
                color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a
 +
                result, the ChBD is well functioned.</p>
 +
            <p></p>
 +
            <div class="row">
 +
                <figure class="col-sm-3"><div class="thumbnail">
 +
                    <img src='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'
 +
                        alt='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'/>
 +
                    <p>[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4,
 +
                        water]</p></div></figure>
 +
                <p></p>
 +
 +
            </div>
 +
  
 
             <br>
 
             <br>

Latest revision as of 03:49, 2 November 2017


Project

Parts

Name Type Description Designer Length
BBa_K2239001 Generator ChBD-7alphaHSDH Ruohong Wang 1089
BBa_K2239002 Generator ChBD-7betaHSDH Ruohong Wang 1179
BBa_K2239003 Generator ChBD-LDH Ruohong Wang 1323
BBa_K2239004 Generator ChBD-GDH Ruohong Wang 1107
BBa_K2239005 Generator ChBD-GFP Ruohong Wang 1047
BBa_K2239006 Generator CBD-7alphaHSDH Ruohong Wang 1080
BBa_K2239007 Generator CBD-7betaHSDH Ruohong Wang 1170
BBa_K2239008 Generator CBD-LDH Ruohong Wang 1314
BBa_K2239009 Generator CBD-GDH Ruohong Wang 1098
BBa_K2239010 Generator CBD-GFP Ruohong Wang 1038
BBa_K2239011 Coding 7alpha-HSDH Ruohong Wang 780
BBa_K2239012 Coding 7beta-HSDH Ruohong Wang 870
BBa_K2239013 Coding GFP Ruohong Wang 738
BBa_K2239014 Coding GDH (glucose dehydrogenas) Ruohong Wang 798
BBa_K2239015 Coding LDH ( lactate dehydrogenase) Ruohong Wang 1014

Contribution

We improved the description of CBD and ChBD on previous part pages.

Proof of the CBD function

The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).

https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png

[Fig. 1. GFP-CBD on gauze before washing]

https://static.igem.org/mediawiki/2017/d/de/2_CBD.png

[Fig. 2. GFP-CBD on gauze after washing]

https://static.igem.org/mediawiki/2017/7/70/3_CBD.png

[Fig. 3. GFP-ChBD on gauze before washing]

https://static.igem.org/mediawiki/2017/1/13/4_CBD.png

[Fig. 4. GFP-ChBD on gauze after washing]

Proof of the ChBD function

The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but without chitin, solution with the presence of GFP and chitin, and water in comparison, are presented.

Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a result, the ChBD is well functioned.

https://static.igem.org/mediawiki/2017/0/07/5_CBD.png

[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4, water]