Difference between revisions of "Team:SDSZ-China/Parts"

 
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                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
−
                     <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li>
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                     <li><a href="/Team:SDSZ-China/Hardware">Hardware</a></li>
 
                 </ul>
 
                 </ul>
 
                 <ul class="nav navbar-nav navbar-right">
 
                 <ul class="nav navbar-nav navbar-right">
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                     style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
 
                     style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
 
                     <li><a href="#Parts">Parts</a></li>
 
                     <li><a href="#Parts">Parts</a></li>
 +
                    <li><a href="#Contribution">Contribution</a></li>
 
                 </ul>
 
                 </ul>
 
             </div>
 
             </div>
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         <div class="col-sm-10" style="padding-right:5%">
 
         <div class="col-sm-10" style="padding-right:5%">
  
 +
            <h1 id="Parts">Parts</h1>
 
             <div>
 
             <div>
 
                 <table>
 
                 <table>
−
<thead>
+
                    <thead>
−
<tr><th>Name</th><th>Type</th><th>Description</th><th>Designer</th><th>Length</th></tr></thead>
+
                    <tr>
−
<tbody><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239001'>BBa_K2239001</a></td><td>Generator</td><td>ChBD-7alphaHSDH</td><td>Ruohong Wang</td><td>1089</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239002'>BBa_K2239002</a></td><td>Generator</td><td>ChBD-7betaHSDH</td><td>Ruohong Wang</td><td>1179</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239003'>BBa_K2239003</a></td><td>Generator</td><td>ChBD-LDH</td><td>Ruohong Wang</td><td>1323</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239004'>BBa_K2239004</a></td><td>Generator</td><td>ChBD-GDH</td><td>Ruohong Wang</td><td>1107</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239005'>BBa_K2239005</a></td><td>Generator</td><td>ChBD-GFP</td><td>Ruohong Wang</td><td>1047</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239006'>BBa_K2239006</a></td><td>Generator</td><td>CBD-7alphaHSDH</td><td>Ruohong Wang</td><td>1080</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239007'>BBa_K2239007</a></td><td>Generator</td><td>CBD-7betaHSDH</td><td>Ruohong Wang</td><td>1170</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239008'>BBa_K2239008</a></td><td>Generator</td><td>CBD-LDH</td><td>Ruohong Wang</td><td>1314</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239009'>BBa_K2239009</a></td><td>Generator</td><td>CBD-GDH</td><td>Ruohong Wang</td><td>1098</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239010'>BBa_K2239010</a></td><td>Generator</td><td>CBD-GFP</td><td>Ruohong Wang</td><td>1038</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011'>BBa_K2239011</a></td><td>Coding</td><td>7alpha-HSDH</td><td>Ruohong Wang</td><td>780</td></tr><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239012'>BBa_K2239012</a></td><td>Coding</td><td>7beta-HSDH</td><td>Ruohong Wang</td><td>870</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239013'>BBa_K2239013</a></td><td>Coding</td><td>GFP</td><td>Ruohong Wang</td><td>738</td></tr><tr><td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239014'>BBa_K2239014</a></td><td>Coding</td><td>GDH (glucose dehydrogenas)</td><td>Ruohong Wang</td><td>798</td></tr><tr><td> <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239015'>BBa_K2239015</a></td><td>Coding</td><td>LDH ( lactate dehydrogenase)</td><td>Ruohong Wang</td><td>1014</td></tr></tbody>
+
                        <th>Name</th>
−
</table>
+
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designer</th>
 +
                        <th>Length</th>
 +
                    </tr>
 +
                    </thead>
 +
                    <tbody>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239001'>BBa_K2239001</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1089</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239002'>BBa_K2239002</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1179</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239003'>BBa_K2239003</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1323</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239004'>BBa_K2239004</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1107</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239005'>BBa_K2239005</a></td>
 +
                        <td>Generator</td>
 +
                        <td>ChBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1047</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239006'>BBa_K2239006</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7alphaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1080</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239007'>BBa_K2239007</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-7betaHSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1170</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239008'>BBa_K2239008</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-LDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1314</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239009'>BBa_K2239009</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1098</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239010'>BBa_K2239010</a></td>
 +
                        <td>Generator</td>
 +
                        <td>CBD-GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1038</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011'>BBa_K2239011</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7alpha-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>780</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239012'>BBa_K2239012</a></td>
 +
                        <td>Coding</td>
 +
                        <td>7beta-HSDH</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>870</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239013'>BBa_K2239013</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GFP</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>738</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239014'>BBa_K2239014</a></td>
 +
                        <td>Coding</td>
 +
                        <td>GDH (glucose dehydrogenas)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>798</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239015'>BBa_K2239015</a></td>
 +
                        <td>Coding</td>
 +
                        <td>LDH ( lactate dehydrogenase)</td>
 +
                        <td>Ruohong Wang</td>
 +
                        <td>1014</td>
 +
                    </tr>
 +
                    </tbody>
 +
                </table>
 
             </div>
 
             </div>
  
−
<div id="bodyContent">
+
            <h1 id="Contribution">Contribution</h1>
−
             <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p><br>
+
             <p>We improved the description of CBD and ChBD on previous part pages.</p>
−
<span style="font-size: 150%; font-weight: bold;">7alpha-HSDH</span>
+
            <h2>Proof of the CBD function</h2>
−
</p><p>7alpha-HSDH (7alpha-hydroxysteroid dehydrogenase) catalyzes the oxidation of hydroxysteroids at C-7 position and back, as it converts NAD+ to NADH and back. It catalyzes the oxidation of CDCA into intermediate product 7-oxo-LAC (7-ketolithocholic acid), where the hydroxyl at C-7 position becomes carbonyl.[1]
+
            <p>The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is
−
</p><p><br>
+
                recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not
−
<span class="h3bb">Sequence and Features</span>
+
                significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is
−
</p>
+
                left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).</p>
−
<script src="http://parts.igem.org/cgi/partsdb/seq_edit/all.js"></script> <div id="sequencePaneDiv" style="clear: both; display: block; top: 0px; left: 0px; z-index: 20; background-color: white; border: 1px solid gray;"> <input type="hidden" id="new_dna_format" name="new_dna_format" value="_ruler_"> <input type="hidden" id="selection_start" name="selection_start" value="1"> <input type="hidden" id="selection_end" name="selection_end" value="0"><form id="saveForm" action="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2239011" method="POST" style="display: none;"><input type="hidden" id="primaryPartId" name="primaryPartId" value="43571"><input type="hidden" id="seqHidden" name="seqHidden" value="catcatcatcatcatcactttaattctgacaacctgagactcgacggaaaatgcgccatcatcacaggtgcgggtgcaggtattggtaaagaaatcgcca
+
            <div class="row">
−
ttacattcgcgacagctggcgcatctgtggtggtcagtgatattaacgccgacgcagctaaccatgttgtagacgaaattcaacaactgggtggtcaggc
+
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'
−
atttgcctgccgttgtgatattacttccgaacaggaactctctgcactggcagactttgccgtcagtaagctgggtaaagttgatatcctggttaacaac
+
                                              alt='https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png'/>
−
gccggtggcggtggtcctaaaccgtttgatatgccaatggcagattttcgccgtgcttatgaactgaatgtgttttcttttttccatctgtcacaacttg
+
                    <p>[Fig. 1. GFP-CBD on gauze before washing]</p></div></figure>
−
ttgcgccagaaatggaaaaaaatggcggtggcgttattctgaccatcacttctatggcggcagaaaataaaaatataaacatgacttcctatgcatcatc
+
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'
−
taaagctgcggccagtcatctggtcagaaatatggcatttgacctgggtgaaaaaaatattcgggtgaatggcattgcgccgggggcaatattaaccgat
+
                                              alt='https://static.igem.org/mediawiki/2017/d/de/2_CBD.png'/>
−
gccctgaaatccgttattacaccagaaattgaacaaaaaatgttacagcacacgccgatcatacgtctgggccaaccgcaagatattgctaacgcggcgc
+
                    <p>[Fig. 2. GFP-CBD on gauze after washing]</p></div></figure>
−
tgttcctttgctcgcctgctgccagctgggtaagcggacaaattctcaccgtctccggtggtggggtacaggagctcaat
+
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'
−
"><input type="hidden" id="firstHidden" name="firstHidden" value=""><input type="hidden" id="lastHidden" name="lastHidden" value=""></form><div style="position: relative; top: 0px; left: 0px; height: 16px; background-color: rgb(221, 221, 221); padding: 5px; border-bottom: 1px solid gray; font-family: arial, sans-serif;"><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; cursor: pointer; color: rgb(102, 102, 102); text-decoration: none;">Subparts</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif;">|</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: bold; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; cursor: pointer; color: black; text-decoration: none;">Ruler</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif;">|</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; cursor: pointer; color: blue; text-decoration: underline;">SS</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif;">|</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; cursor: pointer; color: blue; text-decoration: underline;">DS</span><span style="display: inline; line-height: 15px; padding-left: 50px; font-style: normal; font-variant: normal; font-weight: bold; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif;">Length:  780 bp</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; float: right; padding-right: 10px; color: blue; cursor: pointer;">Get part sequence.</span><span style="display: inline; line-height: 15px; padding-left: 5px; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 13px; font-family: arial, sans-serif; float: right; padding-right: 10px; color: blue; cursor: pointer;">View plasmid<img src="http://parts.igem.org/images/labgenius_p_icon.png" style="width: 15px; height: 15px; position: relative; top: -2px; padding-left: 5px;"></span></div><span class="Size_Pane" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; color: white;">.                                                                                                  .</span><span class="Ruler_Pane" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; color: black; left: 40px; display: none;">1          11        21        31        41        51        61        71        81        91</span><div style="position: relative; display: block;"><span class="Ruler_Pane" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; display: block;">1          100          200          300          400          500          600          700          800</span><div style="position: relative; left: 40px; height: 9px; width: 764px;"><div style="position: absolute; top: 4px; left: 0px; height: 1px; width: 763px; border-top: 1px solid black;"></div><div style="position: absolute; top: 0px; left: 0px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 19px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 38px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 57px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 76px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 95px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 114px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 134px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 153px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 172px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 191px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 210px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 229px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 248px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 267px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 286px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 305px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 324px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 343px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 362px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 382px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 401px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 420px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 439px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 458px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 477px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 496px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 515px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 534px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 553px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 572px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 591px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 610px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 629px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 649px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 668px; height: 9px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 687px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 706px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 725px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 2px; left: 744px; height: 5px; width: 1px; background-color: black;"></div><div style="position: absolute; top: 0px; left: 763px; height: 9px; width: 1px; background-color: black;"></div></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: block;"><div style="position: relative; height: 10px; background-color: white;"></div><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 53px;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 18px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 25px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 32px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 39px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 46px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 53px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 60px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 67px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 74px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 81px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 88px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 95px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 102px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 109px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 116px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 123px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 130px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 137px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 144px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 151px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 158px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 165px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 172px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 179px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 186px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 193px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 200px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 207px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 214px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 221px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 228px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 235px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 242px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 249px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 256px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 263px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 270px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 277px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 284px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 291px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 298px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 305px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 312px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 319px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 326px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 333px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 340px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 347px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 354px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 361px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 368px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 375px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 382px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 389px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 396px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 403px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 410px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 417px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 424px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 431px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 438px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 445px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 452px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 459px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 466px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 473px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 480px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 487px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 494px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 501px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 508px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 515px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 522px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 529px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 536px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 543px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 550px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 557px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 564px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 571px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 578px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 585px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 592px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 599px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 606px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 613px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 620px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 627px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 634px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 641px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 648px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 655px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 662px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 669px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 676px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 683px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 690px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 697px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 704px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 711px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 718px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 725px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 732px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 739px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 353.55px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div><img src="http://parts.igem.org/images/f_icons/tag_l.png" style="top: 24px; left: 1px; height: 7px; width: 1px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_r.png" style="top: 24px; left: 15px; height: 7px; width: 1px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 24px; left: 2px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 24px; left: 9px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 31px; left: 0px; height: 11px; position: absolute; z-index: 60;">his-tag</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 63px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 63px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">catcatcatc atcatcactt taattctgac aacctgagac tcgacggaaa atgcgccatc atcacaggtg cgggtgcagg tattggtaaa gaaatcgcca<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">1</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">gtagtagtag tagtagtgaa attaagactg ttggactctg agctgccttt tacgcggtag tagtgtccac gcccacgtcc ataaccattt ctttagcggt</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 134px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 141px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 148px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 155px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 162px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 169px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 176px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 183px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 190px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 197px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 204px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 211px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 218px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 225px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 232px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 239px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 246px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 253px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 260px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 267px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 274px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 281px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 288px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 295px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 302px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 309px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 316px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 323px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 330px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 337px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 344px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 351px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 358px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 365px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 372px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 379px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 386px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 393px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 400px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 407px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 414px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 421px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 428px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 435px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 442px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 449px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 456px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 463px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 470px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 477px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 484px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 491px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 498px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 505px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 512px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 519px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 526px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 533px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 540px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 547px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 554px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 561px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 568px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 575px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 582px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 589px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 596px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 603px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 610px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 617px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 624px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 631px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 638px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 645px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 652px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 659px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 666px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 673px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 680px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 421.55px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div><img src="http://parts.igem.org/images/f_icons/tag_l.png" style="top: 0px; left: 1px; height: 7px; width: 1px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_r.png" style="top: 0px; left: 132px; height: 7px; width: 1px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 2px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 9px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 16px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 23px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 30px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 37px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 44px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 51px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 58px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 65px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 72px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 79px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 86px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 93px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 100px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 107px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 114px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 121px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/tag_m.png" style="top: 0px; left: 128px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 49.85px; height: 11px; position: absolute; z-index: 60;">his-tag</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">ttacattcgc gacagctggc gcatctgtgg tggtcagtga tattaacgcc gacgcagcta accatgttgt agacgaaatt caacaactgg gtggtcaggc<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">101</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">aatgtaagcg ctgtcgaccg cgtagacacc accagtcact ataattgcgg ctgcgtcgat tggtacaaca tctgctttaa gttgttgacc caccagtccg</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; 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left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">atttgcctgc cgttgtgata ttacttccga acaggaactc tctgcactgg cagactttgc cgtcagtaag ctgggtaaag ttgatatcct ggttaacaac<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">201</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">taaacggacg gcaacactat aatgaaggct tgtccttgag agacgtgacc gtctgaaacg gcagtcattc gacccatttc aactatagga ccaattgttg</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; 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left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">gccggtggcg gtggtcctaa accgtttgat atgccaatgg cagattttcg ccgtgcttat gaactgaatg tgttttcttt tttccatctg tcacaacttg<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">301</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">cggccaccgc caccaggatt tggcaaacta tacggttacc gtctaaaagc ggcacgaata cttgacttac acaaaagaaa aaaggtagac agtgttgaac</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; 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left: 659px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 666px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 673px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 680px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">ttgcgccaga aatggaaaaa aatggcggtg gcgttattct gaccatcact tctatggcgg cagaaaataa aaatataaac atgacttcct atgcatcatc<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">401</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">aacgcggtct ttaccttttt ttaccgccac cgcaataaga ctggtagtga agataccgcc gtcttttatt tttatatttg tactgaagga tacgtagtag</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 22px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 29px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 36px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 43px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 50px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 57px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 64px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 71px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 78px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 85px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 92px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 99px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 106px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 113px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 120px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 127px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 134px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 141px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 148px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 155px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 162px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 169px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 176px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 183px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 190px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 197px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 204px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 211px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 218px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 225px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 232px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 239px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 246px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 253px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 260px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 267px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 274px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 281px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 288px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 295px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 302px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 309px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 316px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 323px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 330px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 337px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 344px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 351px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 358px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 365px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 372px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 379px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 386px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 393px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 400px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 407px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 414px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 421px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 428px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 435px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 442px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 449px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 456px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 463px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 470px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 477px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 484px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 491px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 498px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 505px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 512px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 519px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 526px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 533px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 540px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 547px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 554px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 561px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 568px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 575px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 582px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 589px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 596px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 603px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 610px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 617px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 624px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 631px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 638px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 645px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 652px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 659px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 666px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 673px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 680px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">taaagctgcg gccagtcatc tggtcagaaa tatggcattt gacctgggtg aaaaaaatat tcgggtgaat ggcattgcgc cgggggcaat attaaccgat<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">501</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">atttcgacgc cggtcagtag accagtcttt ataccgtaaa ctggacccac tttttttata agcccactta ccgtaacgcg gcccccgtta taattggcta</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 22px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 29px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 36px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 43px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 50px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 57px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 64px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 71px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 78px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 85px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 92px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 99px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 106px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 113px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 120px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 127px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 134px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 141px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 148px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 155px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 162px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 169px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 176px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 183px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 190px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 197px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 204px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 211px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 218px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 225px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 232px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 239px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 246px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 253px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 260px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 267px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 274px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 281px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 288px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 295px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 302px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 309px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 316px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 323px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 330px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 337px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 344px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 351px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 358px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 365px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 372px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 379px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 386px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 393px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 400px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 407px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 414px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 421px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 428px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 435px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 442px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 449px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 456px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 463px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 470px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 477px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 484px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 491px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 498px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 505px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 512px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 519px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 526px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 533px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 540px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 547px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 554px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 561px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 568px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 575px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 582px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 589px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 596px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 603px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 610px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 617px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 624px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 631px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 638px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 645px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 652px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 659px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 666px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 673px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 680px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">gccctgaaat ccgttattac accagaaatt gaacaaaaaa tgttacagca cacgccgatc atacgtctgg gccaaccgca agatattgct aacgcggcgc<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">601</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">cgggacttta ggcaataatg tggtctttaa cttgtttttt acaatgtcgt gtgcggctag tatgcagacc cggttggcgt tctataacga ttgcgccgcg</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: 765px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 22px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 29px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 36px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 43px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 50px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 57px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 64px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 71px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 78px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 85px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 92px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 99px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 106px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 113px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 120px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 127px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 134px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 141px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 148px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 155px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 162px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 169px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 176px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 183px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 190px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 197px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 204px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 211px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 218px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 225px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 232px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 239px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 246px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 253px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 260px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 267px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 274px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 281px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 288px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 295px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 302px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 309px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 316px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 323px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 330px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 337px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 344px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 351px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 358px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 365px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 372px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 379px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 386px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 393px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 400px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 407px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 414px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 421px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 428px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 435px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 442px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 449px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 456px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 463px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 470px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 477px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 484px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 491px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 498px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 505px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 512px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 519px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 526px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 533px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 540px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 547px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 554px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 561px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 568px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 575px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 582px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 589px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 596px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 603px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 610px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 617px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 624px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 631px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 638px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 645px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 652px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 659px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 666px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 673px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 680px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 687px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 694px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 701px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 708px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 715px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 722px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 729px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 736px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 743px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 750px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 757px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 355.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div class="Row_Pane" style="position: relative; top: 0px; left: 0px; z-index: 40; background-color: white; display: none;"><div style="position: relative; height: 10px; background-color: white;"></div><span class="BP_seqDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">tgttcctttg ctcgcctgct gccagctggg taagcggaca aattctcacc gtctccggtg gtggggtaca ggagctcaat<div class="BP_indexDiv" style="position: absolute; top: 0px; left: -40px; z-index: 60; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; display: none;">701</div></span><span class="BP_compDiv" style="position: relative; white-space: pre; font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; font-size: 12px; line-height: 12px; font-family: monospace; left: 40px; z-index: 60; display: none;">acaaggaaac gagcggacga cggtcgaccc attcgcctgt ttaagagtgg cagaggccac caccccatgt cctcgagtta</span><div class="featureDiv" style="position: relative; top: 1px; left: 40px; width: 100px; z-index: 60; height: 29px;"><img src="http://parts.igem.org/images/f_icons/arrow_r.png" style="top: 0px; left: -8px; height: 5px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 1px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 8px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 15px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 22px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 29px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 36px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 43px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 50px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 57px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 64px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 71px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 78px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 85px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 92px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 99px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 106px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 113px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 120px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 127px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 134px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 141px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 148px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 155px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 162px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 169px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 176px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 183px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 190px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 197px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 204px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 211px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 218px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 225px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 232px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 239px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 246px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 253px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 260px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 267px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 274px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 281px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 288px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 295px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 302px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 309px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 316px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 323px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 330px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 337px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 344px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 351px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 358px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 365px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 372px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 379px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 386px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 393px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 400px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 407px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 414px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 421px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 428px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 435px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 442px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 449px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 456px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 463px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 470px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 477px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 484px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 491px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 498px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 505px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 512px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 519px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 526px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 533px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 540px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 547px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 554px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 561px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 568px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 575px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 582px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 589px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 596px; height: 7px; width: 7px; position: absolute; z-index: 60;"><img src="http://parts.igem.org/images/f_icons/protein_m.png" style="top: 0px; left: 603px; height: 7px; width: 7px; position: absolute; z-index: 60;"><div style="font-size: 85%; white-space: nowrap; top: 7px; left: 278.05px; height: 11px; position: absolute; z-index: 60;">7alpha-HSDH</div></div><div class="Hilite" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 2px; z-index: 50; background-color: rgb(170, 170, 238); visibility: hidden;"></div><div class="Cover_Div" style="position: absolute; top: 0px; left: 0px; height: 39px; width: 943px; z-index: 70;"></div></div><div style="position: relative; height: 60px; display: none;"></div></div> <script> var sequence = new String('catcatcatcatcatcactttaattctgacaacctgagactcgacggaaaatgcgccatcatcacaggtgcgggtgcaggtattggtaaagaaatcgccattacattcgcgacagctggcgcatctgtggtggtcagtgatattaacgccgacgcagctaaccatgttgtagacgaaattcaacaactgggtggtcaggcatttgcctgccgttgtgatattacttccgaacaggaactctctgcactggcagactttgccgtcagtaagctgggtaaagttgatatcctggttaacaacgccggtggcggtggtcctaaaccgtttgatatgccaatggcagattttcgccgtgcttatgaactgaatgtgttttcttttttccatctgtcacaacttgttgcgccagaaatggaaaaaaatggcggtggcgttattctgaccatcacttctatggcggcagaaaataaaaatataaacatgacttcctatgcatcatctaaagctgcggccagtcatctggtcagaaatatggcatttgacctgggtgaaaaaaatattcgggtgaatggcattgcgccgggggcaatattaaccgatgccctgaaatccgttattacaccagaaattgaacaaaaaatgttacagcacacgccgatcatacgtctgggccaaccgcaagatattgctaacgcggcgctgttcctttgctcgcctgctgccagctgggtaagcggacaaattctcaccgtctccggtggtggggtacaggagctcaat');        var seqFeatures = new Array( ['protein',19,780,'7alpha-HSDH', 0], ['tag',1,18,'his-tag', 0]); var subParts = null; var Format = '_ruler_'; var PrimaryPartName = 'BBa_K2239011'; var PrimaryPartID = '43571'; var Selection_Start = 0; var Selection_End = 0; showSeqFeatures(false);  </script><div style="position:relative;clear:both;width:100%"><div style="">
+
                                              alt='https://static.igem.org/mediawiki/2017/7/70/3_CBD.png'/>
−
<p><style type="text/css">
+
                    <p>[Fig. 3. GFP-ChBD on gauze before washing]</p></div></figure>
−
.compatibility_div ul,
+
                <figure class="col-sm-3"><div class="thumbnail"><img src='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'
−
.compatibility_div li {
+
                                              alt='https://static.igem.org/mediawiki/2017/1/13/4_CBD.png'/>
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display: inline;
+
                    <p>[Fig. 4. GFP-ChBD on gauze after washing]</p></div></figure>
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}
+
            </div>
−
.compatibility_div li {
+
            <h2>Proof of the ChBD function</h2>
−
position: relative;
+
            <p>The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but
−
padding-top: 2px;
+
                without chitin, solution with the presence of GFP and chitin, and water in comparison, are
−
padding-left:4px;
+
                presented.</p>
−
padding-right:3px;
+
            <p>Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder
−
margin-right:2px;
+
                inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in
−
margin-bottom: 5px;
+
                tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the
−
}
+
                color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a
−
.compatibility_div .box {
+
                result, the ChBD is well functioned.</p>
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top:  35px;
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            <p></p>
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width: 200px;
+
            <div class="row">
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left:  0px;
+
                <figure class="col-sm-3"><div class="thumbnail">
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}
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                    <img src='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'
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.compatibility_div .box_white {
+
                        alt='https://static.igem.org/mediawiki/2017/0/07/5_CBD.png'/>
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border: 1px solid gray;
+
                    <p>[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4,
−
background-color: white;
+
                        water]</p></div></figure>
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}
+
                <p></p>
−
.compatibility_div .box_red {
+
−
border: 1px solid #dd6666;
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background-color: #ffcccc;
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background-image: url('http://parts.igem.org/images/red_not/red_box.png');
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background-repeat: none;
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}
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.compatibility_div .box_green {
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border: 1px solid #44ee44;
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background-color: #aaffaa;
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}
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</p><p><br />
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</p>
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</style></p><div class="compatibility_div" style="display:inline">
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−
Assembly Compatibility:<ul>
+
−
 
+
−
<li class="boxctrl box_green">10<div class="box"><div>COMPATIBLE WITH RFC[10]</div></div></li>
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<li class="boxctrl box_green">12<div class="box"><div>COMPATIBLE WITH RFC[12]</div></div></li>
+
−
<li class="boxctrl box_green">21<div class="box"><div>COMPATIBLE WITH RFC[21]</div></div></li>
+
−
<li class="boxctrl box_green">23<div class="box"><div>COMPATIBLE WITH RFC[23]</div></div></li>
+
−
<li class="boxctrl box_green">25<div class="box"><div>COMPATIBLE WITH RFC[25]</div></div></li>
+
−
<li class="boxctrl box_green">1000<div class="box"><div>COMPATIBLE WITH RFC[1000]</div></div></li>
+
−
</ul>
+
−
</div></div></div>
+
−
<p><br>
+
−
</p>
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−
<!--  
+
−
NewPP limit report
+
−
CPU time usage: 0.009 seconds
+
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Real time usage: 0.426 seconds
+
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Preprocessor visited node count: 8/1000000
+
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Preprocessor generated node count: 54/1000000
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Post‐expand include size: 0/2097152 bytes
+
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Template argument size: 0/2097152 bytes
+
−
Highest expansion depth: 2/40
+
−
Expensive parser function count: 0/100
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Latest revision as of 03:49, 2 November 2017


Project

Parts

Name Type Description Designer Length
BBa_K2239001 Generator ChBD-7alphaHSDH Ruohong Wang 1089
BBa_K2239002 Generator ChBD-7betaHSDH Ruohong Wang 1179
BBa_K2239003 Generator ChBD-LDH Ruohong Wang 1323
BBa_K2239004 Generator ChBD-GDH Ruohong Wang 1107
BBa_K2239005 Generator ChBD-GFP Ruohong Wang 1047
BBa_K2239006 Generator CBD-7alphaHSDH Ruohong Wang 1080
BBa_K2239007 Generator CBD-7betaHSDH Ruohong Wang 1170
BBa_K2239008 Generator CBD-LDH Ruohong Wang 1314
BBa_K2239009 Generator CBD-GDH Ruohong Wang 1098
BBa_K2239010 Generator CBD-GFP Ruohong Wang 1038
BBa_K2239011 Coding 7alpha-HSDH Ruohong Wang 780
BBa_K2239012 Coding 7beta-HSDH Ruohong Wang 870
BBa_K2239013 Coding GFP Ruohong Wang 738
BBa_K2239014 Coding GDH (glucose dehydrogenas) Ruohong Wang 798
BBa_K2239015 Coding LDH ( lactate dehydrogenase) Ruohong Wang 1014

Contribution

We improved the description of CBD and ChBD on previous part pages.

Proof of the CBD function

The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).

https://static.igem.org/mediawiki/2017/f/f4/1_CBD.png

[Fig. 1. GFP-CBD on gauze before washing]

https://static.igem.org/mediawiki/2017/d/de/2_CBD.png

[Fig. 2. GFP-CBD on gauze after washing]

https://static.igem.org/mediawiki/2017/7/70/3_CBD.png

[Fig. 3. GFP-ChBD on gauze before washing]

https://static.igem.org/mediawiki/2017/1/13/4_CBD.png

[Fig. 4. GFP-ChBD on gauze after washing]

Proof of the ChBD function

The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but without chitin, solution with the presence of GFP and chitin, and water in comparison, are presented.

Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a result, the ChBD is well functioned.

https://static.igem.org/mediawiki/2017/0/07/5_CBD.png

[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4, water]