Difference between revisions of "Team:IISc-Bangalore/Demonstrate"

(Prototype team page)
 
 
(8 intermediate revisions by 4 users not shown)
Line 3: Line 3:
 
<html>
 
<html>
  
 +
<script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script>
 +
<head></head>
  
  
−
<div class="column full_size judges-will-not-evaluate">
+
            <ol id="inPageNav">
−
<h3>★  ALERT! </h3>
+
                <li><a href="#gvstructure">Chitosan enhances flotation<img src="https://static.igem.org/mediawiki/2017/6/68/T--IISc-Bangalore--navbar_bullet.png" /></a></li>
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
−
</div>
+
−
<div class="clear"></div>
+
  
 +
            </ol>
  
−
<div class="column full_size">
 
−
<h1>Demonstrate</h1>
 
−
<h3>Gold Medal Criterion #4</h3>
 
  
−
<p>
+
<div id="contentMain">
−
Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
+
<img src="https://static.igem.org/mediawiki/2017/a/ac/T--IISc-Bangalore--Header--Demo.svg" id="headerImg" />
−
</p>
+
  
−
<p>
+
<h1> Chitosan makes gas vesicles float!</h1>
−
Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
+
−
</p>
+
−
 
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column half_size">
+
−
 
+
−
<h4> What should we do for our demonstration?</h4>
+
−
 
+
−
<h5> Standard teams </h5>
+
−
 
+
−
<p>
+
−
If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
−
</p>
+
−
</div>
+
  
−
<div class="column half_size">
+
<p> Addition of even small quantities of chitosan led to a notable increase in the hydrodynamic radius of the gas vesicles and a significant effect on the rate of decrease of optical density. To verify that the gas vesicles were actually floating, images of gas vesicle suspensions were taken at regular intervals over two hours.</p>
  
−
<br>
+
<p>While native gas vesicles retained an almost uniform distribution throughout the column, the suspension containing chitosan treated gas vesicles quickly cleared up. The images that follow were taken at ten minute time intervals for two hours.</p>
−
<h5> Special track teams </h5>
+
  
−
<p>
+
<figure>
−
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
<img src="https://static.igem.org/mediawiki/2017/5/5a/T--IISc-Bangalore--Demo-native.png" width=800px>
−
</p>
+
<figurecaption>Native gas vesicles tend to distribute themselves uniformly throughout the column owing to strong diffusive forces</figurecaption>
 +
<img src="https://static.igem.org/mediawiki/2017/8/86/T--IISc-Bangalore--Demo-chito.png" width=800px>
 +
<figurecaption>Gas vesicles treated with chitosan cleared up to the top of the column within a few minutes</figurecaption>
 +
</figure>
  
 +
<p>Evidently, flocculation of gas vesicles using chitosan is a rapid, effective method to aggregate gas vesicles, increase their hydrodynamic radius, and cause them to float.</p>
  
 
</div>
 
</div>
  
 +
<script>
 +
window.slide = new SlideNav({
 +
  changeHash: true
 +
});
 +
var height = $('#headerImg').height();
 +
    window.onscroll = function() {myFunction()};
  
 +
    function myFunction() {
 +
        if (document.body.scrollTop > height || document.documentElement.scrollTop > height) {
 +
            $("#inPageNav").fadeIn(200);
 +
        } else {
 +
            $("#inPageNav").fadeOut(200);
 +
        }
 +
    }
 +
</script>
  
 
</html>
 
</html>

Latest revision as of 03:57, 2 November 2017

  1. Chitosan enhances flotation

Chitosan makes gas vesicles float!

Addition of even small quantities of chitosan led to a notable increase in the hydrodynamic radius of the gas vesicles and a significant effect on the rate of decrease of optical density. To verify that the gas vesicles were actually floating, images of gas vesicle suspensions were taken at regular intervals over two hours.

While native gas vesicles retained an almost uniform distribution throughout the column, the suspension containing chitosan treated gas vesicles quickly cleared up. The images that follow were taken at ten minute time intervals for two hours.

Native gas vesicles tend to distribute themselves uniformly throughout the column owing to strong diffusive forces Gas vesicles treated with chitosan cleared up to the top of the column within a few minutes

Evidently, flocculation of gas vesicles using chitosan is a rapid, effective method to aggregate gas vesicles, increase their hydrodynamic radius, and cause them to float.