Difference between revisions of "Team:SJTU-BioX-Shanghai/Composite Part-cn"
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Latest revision as of 10:56, 10 December 2017
复合元件
总体介绍
我们很高兴能为大家呈现这个暑假我们构架的所有关于多因子可视化检测的整合模块
我们的多因子可视化检测系统是怎么工作的？
Figure 1. The STAR-chromoprotein construct.
大量的色素蛋白可以添加到Target的下游，在这里我们使用amilGFP, amilCP, eforRed, cjBlue这四种色素蛋白。为了响应外界环境的变化，我们需要选择适当的启动子，添加到Target的前面。当想要检测的因子不存在时，Antisense不会被表达，Target中的发卡结构也就不会被打开，下游的色素蛋白就不会表达。当外界因子存在时，响应的启动子就会被激活，Antisense转录进而打开Target中的发卡结构，下游的色素蛋白基因得以表达，颜色显现。
两个基因回路被构建在同一个质粒上，我们使用双表达质粒pCDF-Duet1和pET-Duet1。常表达启动子J23119和Target是提交到公司一起合成的，Antisense和它下游的终止子t500也一样。因为我们的系统有许多优点，所以我们有信心这个系统作为荧光蛋白的替代品会被广泛使用。
主要优点:
- STAR1和STAR3系统具有很强的正交性，彼此互不影响；
- 色素蛋白可被肉眼看到，不需要借助如紫外灯等额外设备；
- 我们创造了与之搭配的手机支架和颜色检测APP以更加方便根据颜色得到因子浓度；
- 更多关于STAR的好处已经在帝国理工项目中作了讨论（更多信息）.
Parts汇总表
Reference
- Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs. Nat Chem Biol 11:214–220.
- Meyer, S., Chappell, J., Sankar, S., Chew, R., and Lucks, J. B. (2016) Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies Biotechnol. Bioeng. 113, 216.
