Difference between revisions of "Team:Fudan/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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        <a href="https://2017.igem.org/Team:Fudan/Demonstrate"><img class="align" id="align-1" src='https://static.igem.org/mediawiki/2017/9/93/T--Fudan--synNotch.png' /></a>
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        <a href="https://2017.igem.org/Team:Fudan/Basic_Part"><img class="align" id="align-4" src='https://static.igem.org/mediawiki/2017/2/24/T--Fudan--hccOrange.png' /></a>
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        <a href="https://2017.igem.org/Team:Fudan/Composite_Part"><img class="align" id="align-3" src='https://static.igem.org/mediawiki/2017/6/66/T--Fudan--synTF.png' /></a>
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        <a href="https://2017.igem.org/Team:Fudan/Part_Collection"><img class="align" id="align-2" src='https://static.igem.org/mediawiki/2017/0/0c/T--Fudan--stripe.png' /></a>
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  <h class="sup-title dark-blue"></br>Composite part – SynPro(S)-ZF43-8;8*</br></h>
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  <h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite composite part is SynPro(S)-43-8;8* </br>(BBa_K2446028).</h>
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  <p></br>This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
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</p>
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<div class="block main-text main-text">
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  <h class="highlight-title dark-blue" id="Antigen"></br></br>How SynPro(S)-ZF43-8;8*works </h>
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  <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats. 
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<h1>Composite Parts</h1>
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<img width="30%" src="https://static.igem.org/mediawiki/2017/9/91/T--Fudan--CPFigure.jpg">
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<div class="block main-text main-text">
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  <h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h>
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  <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
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</p>
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<p>
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The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
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</p>
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<div class="block main-text main-text">
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  <h class="highlight-title dark-blue" id="Antigen"></br></br>See More</h>
 
<p>
 
<p>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website.
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</p>
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<p>
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We listed other composite part we constructed this year below.  
 
</p>
 
</p>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Best Composite Part Special Prize</h3>
 
  
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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    <td>SV40_8_ZF_42-10 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446025>(BBa_K2446025)</a></td>
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  </tr>
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    <td>SV40_2_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446026>(BBa_K2446026)</a></td>
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  </tr>
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    <td>SV40_4_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446027>(BBa_K2446027)</a></td>
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  </tr>
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    <td>SV40_8_ZF_54-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446029>(BBa_K2446029)</a></td>
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  </tr>
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    <td>SV40_8_ZF_21-16 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446030>(BBa_K2446030)</a></td>
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  </tr>
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    <td>SV40_8_ZF54-8/43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446031>(BBa_K2446031)</a></td>
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  </tr>
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    <td>SV40_4_ZFHD1 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446032>(BBa_K2446032)</a></td>
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  </tr>
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    <td>SV40_2_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446033>(BBa_K2446033)</a></td>
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  </tr>
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    <td>SV40_4_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446034>(BBa_K2446034)</a></td>
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  </tr>
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    <td>SV40_8_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446035>(BBa_K2446035)</a></td>
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  </tr>
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    <td>SV40_UAS promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446036>(BBa_K2446036)</a></td>
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  </tr>
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    <td>CMV_8_ZF_42-10 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446018>(BBa_K2446018)</a></td>
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    <td>CMV_8_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446019>(BBa_K2446019)</a></td>
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  </tr>
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    <td>CMV_8_ZF_54-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446020>(BBa_K2446020)</a></td>
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  </tr>
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    <td>CMV_8_ZF54-8/43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446021>(BBa_K2446021)</a></td>
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    <td>CMV_4_ZFHD1 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446022>(BBa_K2446022)</a></td>
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  </tr>
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    <td>CMV_4_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446023>(BBa_K2446023)</a></td>
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  </tr>
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    <td>CMV_8_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446024>(BBa_K2446024)</a></td>
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{{Fudan/PageFooter}}

Latest revision as of 11:53, 14 December 2017

Composite part – SynPro(S)-ZF43-8;8*


For this year’s iGEM competition, our favorite composite part is SynPro(S)-43-8;8*
(BBa_K2446028).


This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).



How SynPro(S)-ZF43-8;8*works

The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.



The advantages of pSV40-ZF43-8;8*

Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.

The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.



See More

Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.

We listed other composite part we constructed this year below.

SV40_8_ZF_42-10 promoter (BBa_K2446025)
SV40_2_ZF_43-8 promoter (BBa_K2446026)
SV40_4_ZF_43-8 promoter (BBa_K2446027)
SV40_8_ZF_54-8 promoter (BBa_K2446029)
SV40_8_ZF_21-16 promoter (BBa_K2446030)
SV40_8_ZF54-8/43-8 promoter (BBa_K2446031)
SV40_4_ZFHD1 promoter (BBa_K2446032)
SV40_2_PIP promoter (BBa_K2446033)
SV40_4_PIP promoter (BBa_K2446034)
SV40_8_PIP promoter (BBa_K2446035)
SV40_UAS promoter (BBa_K2446036)
CMV_8_ZF_42-10 promoter (BBa_K2446018)
CMV_8_ZF_43-8 promoter (BBa_K2446019)
CMV_8_ZF_54-8 promoter (BBa_K2446020)
CMV_8_ZF54-8/43-8 promoter (BBa_K2446021)
CMV_4_ZFHD1 promoter (BBa_K2446022)
CMV_4_PIP promoter (BBa_K2446023)
CMV_8_PIP promoter (BBa_K2446024)