Difference between revisions of "Team:Fudan/Composite Part"

 
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   <h class="sup-title dark-blue">Composite part – SynPro(S)-ZF43-8;8*</br></h>
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   <h class="sup-title dark-blue"></br>Composite part – SynPro(S)-ZF43-8;8*</br></h>
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  <!--<h class="sub-title dark-blue">enables antigen density dependent tri-response for therapeutic applications</h>-->
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<div class="block main-text main-text">
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   <h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite basic part is SynPro(S)-43-8;8* </br>(BBa_K2446028).</h>
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   <h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite composite part is SynPro(S)-43-8;8* </br>(BBa_K2446028).</h>
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   <p></br>This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S). </br></br>
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   <p></br>This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).
 
</p>
 
</p>
 
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</div>
  
 
<div class="block main-text main-text">
 
<div class="block main-text main-text">
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   <h class="highlight-title dark-blue" id="Antigen"></br></br>How pSV40-ZF43-8;8*works </h>
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   <h class="highlight-title dark-blue" id="Antigen"></br></br>How SynPro(S)-ZF43-8;8*works </h>
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   <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ end to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.   
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   <p>The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.   
 
</p>
 
</p>
  
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<img width="30%" src="https://static.igem.org/mediawiki/2017/9/91/T--Fudan--CPFigure.jpg">
 
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<div class="block main-text main-text">
 
<div class="block main-text main-text">
 
   <h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h>
 
   <h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h>
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   <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression in constant expression state is high. Meanwhile, it is almost being completely silenced in silencing expression state.
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   <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
 
</p>
 
</p>
 
<p>
 
<p>
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The silencing fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
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The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.
 
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<div class="block main-text main-text">
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  <h class="highlight-title dark-blue" id="Antigen"></br></br>See More</h>
 
<p>
 
<p>
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Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website.
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Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website.
 
</p>
 
</p>
 
<p>
 
<p>
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We listed all the composite parts we constructed this year here.  
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We listed other composite part we constructed this year below.  
 
</p>
 
</p>
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<table class="tg" width="40%" style="border-collapse:separate; border-spacing:20px 10px;>
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  <colgroup span="1" width="50%"></colgroup>
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  <colgroup span="1" width="50%"></colgroup>
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  <tr>
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    <td>SV40_8_ZF_42-10 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446025>(BBa_K2446025)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_2_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446026>(BBa_K2446026)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_4_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446027>(BBa_K2446027)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_8_ZF_54-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446029>(BBa_K2446029)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_8_ZF_21-16 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446030>(BBa_K2446030)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_8_ZF54-8/43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446031>(BBa_K2446031)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_4_ZFHD1 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446032>(BBa_K2446032)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_2_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446033>(BBa_K2446033)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_4_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446034>(BBa_K2446034)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_8_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446035>(BBa_K2446035)</a></td>
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  </tr>
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  <tr>
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    <td>SV40_UAS promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446036>(BBa_K2446036)</a></td>
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  </tr>
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  <tr>
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    <td>CMV_8_ZF_42-10 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446018>(BBa_K2446018)</a></td>
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  </tr>
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  <tr>
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    <td>CMV_8_ZF_43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446019>(BBa_K2446019)</a></td>
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  </tr>
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  <tr>
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    <td>CMV_8_ZF_54-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446020>(BBa_K2446020)</a></td>
 +
  </tr>
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  <tr>
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    <td>CMV_8_ZF54-8/43-8 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446021>(BBa_K2446021)</a></td>
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  </tr>
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  <tr>
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    <td>CMV_4_ZFHD1 promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446022>(BBa_K2446022)</a></td>
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  </tr>
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  <tr>
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    <td>CMV_4_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446023>(BBa_K2446023)</a></td>
 +
  </tr>
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  <tr>
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    <td>CMV_8_PIP promoter</td>
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    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446024>(BBa_K2446024)</a></td>
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  </tr>
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Latest revision as of 11:53, 14 December 2017

Composite part – SynPro(S)-ZF43-8;8*


For this year’s iGEM competition, our favorite composite part is SynPro(S)-43-8;8*
(BBa_K2446028).


This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).



How SynPro(S)-ZF43-8;8*works

The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.



The advantages of pSV40-ZF43-8;8*

Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.

The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.



See More

Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.

We listed other composite part we constructed this year below.

SV40_8_ZF_42-10 promoter (BBa_K2446025)
SV40_2_ZF_43-8 promoter (BBa_K2446026)
SV40_4_ZF_43-8 promoter (BBa_K2446027)
SV40_8_ZF_54-8 promoter (BBa_K2446029)
SV40_8_ZF_21-16 promoter (BBa_K2446030)
SV40_8_ZF54-8/43-8 promoter (BBa_K2446031)
SV40_4_ZFHD1 promoter (BBa_K2446032)
SV40_2_PIP promoter (BBa_K2446033)
SV40_4_PIP promoter (BBa_K2446034)
SV40_8_PIP promoter (BBa_K2446035)
SV40_UAS promoter (BBa_K2446036)
CMV_8_ZF_42-10 promoter (BBa_K2446018)
CMV_8_ZF_43-8 promoter (BBa_K2446019)
CMV_8_ZF_54-8 promoter (BBa_K2446020)
CMV_8_ZF54-8/43-8 promoter (BBa_K2446021)
CMV_4_ZFHD1 promoter (BBa_K2446022)
CMV_4_PIP promoter (BBa_K2446023)
CMV_8_PIP promoter (BBa_K2446024)