Difference between revisions of "Team:Fudan/Composite Part"

 
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   <h class="sup-title dark-blue">Composite part – SynPro(S)-ZF43-8;8*</br></h>
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   <h class="highlight-title dark-blue" id="Antigen"></br></br>The advantages of pSV40-ZF43-8;8*</h>
 
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   <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basal expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
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   <p>Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.
 
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Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website.
 
Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446028">(BBa_K2446028)</a> on the Registry of Standard Biological Parts website.
 
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We listed all the composite parts we constructed this year <a href="https://2017.igem.org/Team:Fudan/Part_Collection">here</a>.  
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We listed other composite part we constructed this year below.  
 
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Latest revision as of 11:53, 14 December 2017

Composite part – SynPro(S)-ZF43-8;8*


For this year’s iGEM competition, our favorite composite part is SynPro(S)-43-8;8*
(BBa_K2446028).


This part is a silencing-form synthetic promoter (SynPro(S)) which can be silenced by its corresponding silencing-form synthetic transcription factor SynTF(S)-43-8. This composite part has two congeners SynPro(S)-43-8;2* (BBa_K2446026) and SynPro(S)-43-8;4* (BBa_K2446027). They differ in the repeat of response elements on SynPro(S).



How SynPro(S)-ZF43-8;8*works

The SynPro(S)-ZF43-8;8* was designed in this way: it contains two core domains from 5’ to 3’ end: pSV40, ZF43-8 response elements (ZF43-8REs) in 8 repeats. While SynPro(S)-ZF43-8;2*/4* contains 2 or 4 repeats.



The advantages of pSV40-ZF43-8;8*

Without the existing of SynTF(S)-43-8, SynPro(S)-ZF43-8;8* can express constantly. While it would be silenced with the existing of SynTF(S)-43-8. The basic expression level in constant expression state is high. Meanwhile, it is almost completely silenced in silenced expression state.

The reduction fold of SynPro(S)-ZF43-8;N* is tunable by choosing different N* RE repeats.



See More

Integrated information about surEGFP posted on Demonstrate page: Wiring orthogonal and tunable SynTF-SynPro repertoire. Or you can alternatively check the (BBa_K2446028) on the Registry of Standard Biological Parts website.

We listed other composite part we constructed this year below.

SV40_8_ZF_42-10 promoter (BBa_K2446025)
SV40_2_ZF_43-8 promoter (BBa_K2446026)
SV40_4_ZF_43-8 promoter (BBa_K2446027)
SV40_8_ZF_54-8 promoter (BBa_K2446029)
SV40_8_ZF_21-16 promoter (BBa_K2446030)
SV40_8_ZF54-8/43-8 promoter (BBa_K2446031)
SV40_4_ZFHD1 promoter (BBa_K2446032)
SV40_2_PIP promoter (BBa_K2446033)
SV40_4_PIP promoter (BBa_K2446034)
SV40_8_PIP promoter (BBa_K2446035)
SV40_UAS promoter (BBa_K2446036)
CMV_8_ZF_42-10 promoter (BBa_K2446018)
CMV_8_ZF_43-8 promoter (BBa_K2446019)
CMV_8_ZF_54-8 promoter (BBa_K2446020)
CMV_8_ZF54-8/43-8 promoter (BBa_K2446021)
CMV_4_ZFHD1 promoter (BBa_K2446022)
CMV_4_PIP promoter (BBa_K2446023)
CMV_8_PIP promoter (BBa_K2446024)