Difference between revisions of "Team:RPI Troy NY/Results"

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{{RPI_Troy_NY}}
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<a href="https://2017.igem.org/Team:RPI_Troy_NY"style="text-decoration:none;color:#FFFAF0">Home </a> </td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/Team" style="text-decoration:none;color:#FFFAF0">Team </a> </td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/Collaborations" style="text-decoration:none;color:#FFFAF0">Collaborations </a></td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/Description" style="text-decoration:none;color:#FFFAF0">Project</a> </td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/Results" style="text-decoration:none;color:#FFFAF0">Results </a> </td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/InterLab" style="text-decoration:none;color:#FFFAF0">Interlab Study</a></td>
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/HP/Silver" style="text-decoration:none;color:#FFFAF0">Human Practices </a></td>
  
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<a href="https://2017.igem.org/Team:RPI_Troy_NY/Attributions" style="text-decoration:none;color:#FFFAF0">Attributions </a></td>
  
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<center><h1 style="border:3px; border-style:solid; border-color:#323232; padding:20px;width:325px;">
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<font face = "Palatino"><font size = "8">Results</h1></center></font>
  
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<h5>What should this page contain?</h5>
 
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<li> Clearly and objectively describe the results of your work.</li>
 
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<li> Future plans for the project. </li>
 
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<li> Considerations for replicating the experiments. </li>
 
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<h5>You should also describe what your results mean: </h5>
 
  
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<p>After culturing our control yeast strain, one with a full <i>MFE-2</i> gene knockout and one wild-type, it appeared there was not enough separation in the flasks to indicate enough sophorolipid yield. Unforeseen issues with primer design as well as fusion PCR delayed the microbial genetics committee by a few weeks. A new protocol was created by Dr. Gross and was implemented from November 3 through November 9. Final results were presented by the team at the Giant Jamboree.</p>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h3>Preliminary Results</h3>
  
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<h5>Below are previous Sophorolipid LCMS Data collected. This data will no longer be used. Final data to be presented at the Jamboree.</h5>
  
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<h5> Project Achievements </h5>
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<p><img src="https://static.igem.org/mediawiki/2017/a/a6/Rpi_preliminary_results.png" width="900px" height="300px"></p>
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<br> Below are the final data as presented at the Giant Jamboree. Our data demonstrate that selective modification of the <i>Starmerella bombicola</i> (yeast) <i>MFE-2</i> gene improves the efficiency of sophorolipid production.</h5><br>
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<img src="https://static.igem.org/mediawiki/2017/6/67/Data_1.png"><br>
  
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<ul>
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<img src="https://static.igem.org/mediawiki/2017/e/ec/2017_Rpi_try_2.png"><br>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<img src="https://static.igem.org/mediawiki/2017/e/ef/2017_rpi_troy_ny_data_3.png"><br>
  
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<img src="https://static.igem.org/mediawiki/2017/8/8f/2017_rpi_troy_ny_data_4.png"><br>
  
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<img src="https://static.igem.org/mediawiki/2017/7/76/2017_rpi_troy_ny_data_5.png"><br>
  
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<h5>Inspiration</h5>
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<img src="https://static.igem.org/mediawiki/2017/a/a4/2017_rpi_troy_ny_data_6.png"><br>
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Latest revision as of 22:56, 14 December 2017

Home Team Collaborations Project Results
Interlab Study Safety Human Practices Attributions

Results

After culturing our control yeast strain, one with a full MFE-2 gene knockout and one wild-type, it appeared there was not enough separation in the flasks to indicate enough sophorolipid yield. Unforeseen issues with primer design as well as fusion PCR delayed the microbial genetics committee by a few weeks. A new protocol was created by Dr. Gross and was implemented from November 3 through November 9. Final results were presented by the team at the Giant Jamboree.

Preliminary Results

Below are previous Sophorolipid LCMS Data collected. This data will no longer be used. Final data to be presented at the Jamboree.


Below are the final data as presented at the Giant Jamboree. Our data demonstrate that selective modification of the Starmerella bombicola (yeast) MFE-2 gene improves the efficiency of sophorolipid production.