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− | + | body { | |
− | + | position: relative; | |
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} | } | ||
+ | @media (min-width: 768px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:10px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:100px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:8px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | |||
+ | /* 中等屏幕(桌面显示器,大于等于992px) */ | ||
+ | @media (min-width: 992px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:20px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:110px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:10px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
+ | /* 大屏幕(大桌面显示器,大于等于1200px) */ | ||
+ | @media (min-width: 1200px) { | ||
+ | .menu_logo_1 { | ||
+ | top: 18px; | ||
+ | left:80px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .menu_logo_2 { | ||
+ | top: 18px; | ||
+ | left:170px; | ||
+ | z-index: 1000; | ||
+ | position: fixed; | ||
+ | } | ||
+ | .nav-pills{ | ||
+ | font-size:12px; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | } | ||
</style> | </style> | ||
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<ul class="nav nav-pills nav-stacked" style="font-size:12px; text-align:center; background:#ccc;"> | <ul class="nav nav-pills nav-stacked" style="font-size:12px; text-align:center; background:#ccc;"> | ||
<li class="active"><a href="#section1">Sensing part</a></li> | <li class="active"><a href="#section1">Sensing part</a></li> | ||
− | <li><a href="#section2"> | + | <li><a href="#section2">Enrichment part<br> and recycling part</a></li> |
<li><a href="#section3">The whole circuit </a></li> | <li><a href="#section3">The whole circuit </a></li> | ||
<li><a href="#section4">Detection of <br>sensing part</a></li> | <li><a href="#section4">Detection of <br>sensing part</a></li> | ||
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<h4><strong>①Amplify</strong> </h4> | <h4><strong>①Amplify</strong> </h4> | ||
<p> | <p> | ||
− | <I><I><I><I><I><I><I>PmrA-PmrB(Fe)</I></I></I></I></I></I></I>、 <I> <I> <I> <I> PmrC-GFP </I></I></I></I> gene through the pcr protocol | + | <I><I><I><I><I><I><I>PmrA-PmrB(Fe)</I></I></I></I></I></I></I>、 <I> <I> <I> <I> PmrC-GFP </I></I></I></I> gene through the pcr protocol. |
</p> | </p> | ||
<h4><strong>②Plasmid Construction</strong> </h4> | <h4><strong>②Plasmid Construction</strong> </h4> | ||
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<div id="section2" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | <div id="section2" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;"> | ||
− | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;"> | + | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Enrichment part and recycling part </h3> |
<p><strong>The Capture part is to capture the rare earth ions in the environment and to anchor our cells over the silicon net.</strong></p> | <p><strong>The Capture part is to capture the rare earth ions in the environment and to anchor our cells over the silicon net.</strong></p> | ||
<h4><strong>①With appropriate primers, PCR was carried out to amplify target gene <I>oprF-LBT1-12</I> and <I>oprF-Sitag</I>.</strong> </h4> | <h4><strong>①With appropriate primers, PCR was carried out to amplify target gene <I>oprF-LBT1-12</I> and <I>oprF-Sitag</I>.</strong> </h4> | ||
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<h4 class="col-xs-12"><strong>③Detection</strong> </h4> | <h4 class="col-xs-12"><strong>③Detection</strong> </h4> | ||
<p class="col-xs-12"> | <p class="col-xs-12"> | ||
− | In the meanwhile, we added the FLAG tag to | + | In the meanwhile, we added the FLAG tag to test whether the <I>LBTs</I> and the <I>Sitag</I> was expressed on the cell membrane. |
</p> | </p> | ||
</div> | </div> | ||
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<h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Construction of the whole circuit</h3> | <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Construction of the whole circuit</h3> | ||
<p> | <p> | ||
− | <I> PmrA-PmrB | + | <I> PmrA-PmrB(Fe)-PmrC-GFP</I> All of our part should be built in one plasmid. Therefore, we use endonuclease and ligase to replace the <I>GFP</I> by <I>oprF-LBT</I> and <I>oprF-Sitag</I>. |
</p> | </p> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/5/58/2017_HUST_China_Experiment_image4.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/5/58/2017_HUST_China_Experiment_image4.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | ||
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<img title="demo1" src="https://static.igem.org/mediawiki/2017/1/1a/2017_HUST_China_Experiment_image5.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/1/1a/2017_HUST_China_Experiment_image5.png" alt="experiment_circuit" alt="demo1" class="col-xs-4 col-xs-offset-4" style="padding: 10px 0px;"> | ||
− | <p class="col-xs-12">In order to make the terbium ions sufficiently precipitated, we add excess base. Rinse the precipitate and dissolve the precipitate with hydrochloric acid titration. In this way, we can know the amount of terbium ions which have already adsorbed on the surface of the bacteria. | + | <p class="col-xs-12">In order to make the terbium ions sufficiently precipitated, we add excess base. Rinse the precipitate and dissolve the precipitate with 0.1 M hydrochloric acid titration. In this way, we can know the amount of terbium ions which have already adsorbed on the surface of the bacteria. |
</p> | </p> | ||
+ | </div> | ||
− | < | + | |
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <nav class="col-sm-2" id="myScrollspy2.0"> | ||
+ | <div class="container-fluid"> | ||
+ | <div class="collapse navbar-collapse" id="navbar-collapse"> | ||
+ | <ul class="nav nav-pills2 nav-stacked"> | ||
+ | <li class="active"> | ||
+ | <div style="display:none;" id="rocket-to-top"> | ||
+ | <div style="opacity:0;display:block;" class="level-2"></div> | ||
+ | <div class="level-3"></div> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <footer id="footer"> | ||
+ | <div class="container" style="text-align: left"> | ||
+ | <h3 class="col-xs-12"><strong>Acknowledgments:</strong></h3> | ||
+ | <img style="max-width: 100%; height:auto; display: block;" src="https://static.igem.org/mediawiki/2017/8/88/2017_HUST_China_seven_logo.png" /> | ||
+ | </div> | ||
+ | </footer> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 09:09, 15 December 2017