Difference between revisions of "Team:Berlin diagnostX"
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<img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/f/f0/T--Berlin_diagnostX--icon3.png/600px-T--Berlin_diagnostX--icon3.png"> | <img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/f/f0/T--Berlin_diagnostX--icon3.png/600px-T--Berlin_diagnostX--icon3.png"> | ||
<h5 class="igem_blue text-center mt-4 mb-1 pb-1">INSUFFICIENT DRUG DISTRIBUTION</h5> | <h5 class="igem_blue text-center mt-4 mb-1 pb-1">INSUFFICIENT DRUG DISTRIBUTION</h5> | ||
| − | <p class="text-center px-5 pt-2">Although affordable therapeutics exist</p> | + | <p class="text-center px-5 pt-2">Although affordable therapeutics exist, they don't reach patients in infectious hotspots </p> |
</div> | </div> | ||
<div class="col-4 px-3"> | <div class="col-4 px-3"> | ||
<img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/2/2d/T--Berlin_diagnostX--icon2.png/600px-T--Berlin_diagnostX--icon2.png"> | <img class="px-5 img-fluid" src="https://static.igem.org/mediawiki/2017/thumb/2/2d/T--Berlin_diagnostX--icon2.png/600px-T--Berlin_diagnostX--icon2.png"> | ||
<h5 class="igem_blue text-center mt-4 mb-1 pb-1">EXPENSIVE DIAGNOSTICS</h5> | <h5 class="igem_blue text-center mt-4 mb-1 pb-1">EXPENSIVE DIAGNOSTICS</h5> | ||
| − | <p class="text-center px-5 pt-2">No | + | <p class="text-center px-5 pt-2">No methods suited for field use are yet available preventing the identification of infectious hotspots</p> |
</div> | </div> | ||
</div> | </div> | ||
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<hr class="igem_seperator mt-0 ml-0"> | <hr class="igem_seperator mt-0 ml-0"> | ||
<h5 class="igem_blue pb-1 mb-4">Target Identification</h5> | <h5 class="igem_blue pb-1 mb-4">Target Identification</h5> | ||
| − | <p class="text-justify mb-1"> | + | <p class="text-justify mb-1">We sequenced 96 million RNAs and mapped them against about 12.000 Genes of T. saginata, T. asiatica and T. solium. The expression level of unique RNAs was analyzed. We identified 215 unique, potential diagnost-x targets.</p> |
<p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | <p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | ||
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
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<hr class="igem_seperator mt-0 ml-0"> | <hr class="igem_seperator mt-0 ml-0"> | ||
<h5 class="igem_blue pb-1">Sensor Screening</h5> | <h5 class="igem_blue pb-1">Sensor Screening</h5> | ||
| − | <p class="text-justify mb-4">The designed switches from the In Silico Team were | + | <p class="text-justify mb-4">The designed switches from the In Silico Team were assembled using Extension PCR. Assembled Sensors passed all through the standard screening in liquid phase.</p> |
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
<p class="d-inline pl-3"> 6 successfully working sensors detected thanks to our high flow-through Screening Pipeline</p> | <p class="d-inline pl-3"> 6 successfully working sensors detected thanks to our high flow-through Screening Pipeline</p> | ||
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<hr class="igem_seperator mt-0 ml-0"> | <hr class="igem_seperator mt-0 ml-0"> | ||
<h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | <h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | ||
| − | <p class="text-justify mb-4">Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used- therefore we spared no expense and investigated local circumstances in India</p> | + | <p class="text-justify mb-4">Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used - therefore we spared no expense and investigated local circumstances in India.</p> |
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
<p class="d-inline pl-3"> Find out more about what the insight we gained in India!</p> | <p class="d-inline pl-3"> Find out more about what the insight we gained in India!</p> | ||
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<hr class="igem_seperator mt-0 ml-0"> | <hr class="igem_seperator mt-0 ml-0"> | ||
<h5 class="igem_blue pb-1">Raising awareness for T. solium </h5> | <h5 class="igem_blue pb-1">Raising awareness for T. solium </h5> | ||
| − | <p class="text-justify mb-4">Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. Our mission was to raise awareness for NTDs and education of a new generation.</p> | + | <p class="text-justify mb-4">Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. Our mission was to raise awareness for NTDs and provide innovative impulses for the education of a new generation.</p> |
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
<p class="d-inline pl-3"> Organisation of a congress with over 150 scientists, politicians & students </p> | <p class="d-inline pl-3"> Organisation of a congress with over 150 scientists, politicians & students </p> | ||
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Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!</p> | Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!</p> | ||
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
| − | <p class="d-inline pl-3">Development of an <strong>augmented reality | + | <p class="d-inline pl-3">Development of an <strong>augmented reality app </strong> for innovative future education</p> |
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/AR" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/AR" role="button">Discover More</a></div> | ||
</div> | </div> | ||
Latest revision as of 01:15, 16 December 2017
The fight against T. solium
The worldwide fight against Taenia solium (pork tapeworm) is facing three different challenges
HIGH PREVALENCE
Estimated billions are affected - the infection causing epilepsy, blindness and death
INSUFFICIENT DRUG DISTRIBUTION
Although affordable therapeutics exist, they don't reach patients in infectious hotspots
EXPENSIVE DIAGNOSTICS
No methods suited for field use are yet available preventing the identification of infectious hotspots
The vision of diagnost-x
Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium
Target Identification
We sequenced 96 million RNAs and mapped them against about 12.000 Genes of T. saginata, T. asiatica and T. solium. The expression level of unique RNAs was analyzed. We identified 215 unique, potential diagnost-x targets.
The colour intensity is proportional to the expression of each identified possible target.
First RNA isolation from T. solium eggs avoiding hatching procedure
Sensor Design
Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.
Refined preselection of promising sensors thanks to established software
Sensor Screening
The designed switches from the In Silico Team were assembled using Extension PCR. Assembled Sensors passed all through the standard screening in liquid phase.
6 successfully working sensors detected thanks to our high flow-through Screening Pipeline
Considerating future application from Day 1
Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used - therefore we spared no expense and investigated local circumstances in India.
Find out more about what the insight we gained in India!
Raising awareness for T. solium
Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. Our mission was to raise awareness for NTDs and provide innovative impulses for the education of a new generation.
Organisation of a congress with over 150 scientists, politicians & students
Route to the Future
The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!
Development of an augmented reality app for innovative future education
Many Thanks to our Sponsors!
