Difference between revisions of "Team:Berlin diagnostX/Experiments"

 
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            <h3 class="text-center igem_blue mt-4"><strong>Project Management</strong></h3>
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        <h2 class="text-center igem_blue">Project Management</h2>
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        <p class="text-justify mb-3">Our team consists of more than 30 members who are involved into very different parts of the project. Of course, the larger part of the team is involved into the lab work at some point, but other tasks have to be covered: financial resources need to be acquired, collaborations have to be set up, digital solutions demand programming and networking, and last but not least our immense focus on science communication and public relations required some manpower (or womanpower) behind it. </p>
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        <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> <h5 class="d-inline pl-3 my-3"> How can such a large team work?</h5>
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    <p class="mt-5 text-justify"> As a first and most important basic rule, we took the dictum “divide and conquer” to heart: no one can do everything, and even more, no one likes to do everything. Every member of our team thus associated to one or several subteams he or she personally felt most enticing. These subteams included the following affiliations: </p>
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        <li>Science Team, further divided into <ol>
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            <li>In Vitro RNA sensor design (Team 0)</li>
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            <li>Sensor synthesis (Team 1)</li>
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            <li>Sensor screening/Cell free expression (Team 2)</li>
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            </ol></li>
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            <li>Fundraising Team</li>
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            <li>Entrepreneurship Team</li>
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            <li>Public Relations & Collaborations Team</li>
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    <p class="text-justify mb-3">We embraced a strategy of low hierarchies: Henrik Sadlowski was named project leader, and each subteam also had a team leader. However, each team member had high personal responsibility and the authority to independently make decisions in his or her field of expertise. </p>
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    <p class="text-justify">In order to ensure the individual team members in their subteams working as interlocking parts towards one goal, another key feature was required: <strong>communication</strong>. Throughout the months, we set up several interlocking strategies in order to keep information flowing between and within subteams. One part of the communication took place online – Trello, a free web-based project management application, helps to divide tasks and set deadlines. Furthermore, the Science team used Benchling, a life science data management and collaboration platform, as means of a digital lab notebook. The digital communication in place, we also set up strategies for actual face-to-face communication. <br> <br>
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In the first phase of the project, concerned primarily with planning and raising funds, fortnightly team leader meetings would allow for the debates needed to propel an idea towards its realization, while the day-to-day work took place in the subteams. Later, when the project was at full speed and everyone was working on very specific tasks, we replaced the team leader meetings by a “diagnost-x co-working” – every Tuesday and Thursday night, we would work at the same time in the laboratories, offices and even in the kitchen of Prof. Schuelkes facilities. These co-workings ensured many questions could be solved at the same time, not requiring everyone to follow each and every problem.
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You can find out about the detailed work of the Science Team <a class="igem_blue" href="https://2017.igem.org/Team:Berlin_diagnostX/Design">here</a>.
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The Entrepreneur Team shows its work <a class="igem_blue" href="https://2017.igem.org/Team:Berlin_diagnostX/Entrepreneurship">here</a>, and the results of the Collaborations and Public Relations Teams can be found <a class="igem_blue" href="https://2017.igem.org/Team:Berlin_diagnostX/Collaborations">here</a> and <a class="igem_blue" href="https://2017.igem.org/Team:Berlin_diagnostX/Human_Practices">here</a>. In order not to omit a part of work without which this project could not have existed, we lay open our funding strategy below.</p>
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<div class="row mt-5"><div class="col-12"><h3 class="text-center igem_blue">Fundraising: How we financed one year of diagnost-x</h3>
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    <p class="text-justify">The basic principles of our project can be divided into three aspects:</p>
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        <li>Humanitarian Aid</li>
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        <li>Establishment of new technology</li>
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        <li>Encouraging young scientists</li>
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    </ul>
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    <p class="text-justify mt-3">All of these aspects are of interest for different technological as well as charitable institutions. <br>
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We approached these institutions by presenting on the one hand the global challenge of T.solium infection and on the other hand the opportunities that go along with the toehold switch technology. In this respect, we aimed to show potential sponsors and promoters how this technology could revolutionize field diagnostics – and to present the advantages of a team consisting of young, motivated scientists who are not yet primed to think inside their silos. <br> <br>
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By this approach, we were able to convince different institutions to sponsor different aspects of our project. Whereas corporates of the biotechnological sector supported us with equipment, finance and consulting corporates were convinced of our team structure, and would support our participation in the iGEM competition. Institutional funding would often be given for international collaborations such as our project with India, or our participation in the Cystinet conference. </p>
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             <li>Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li>
 
             <li>Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li>
 
         </ol></li>
 
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        <p class="mt-4"><strong>Please find detailed protocols under the "Protocol" Section</strong></p>      
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    <p class="mt-4"><strong>Please find detailed protocols under the "Protocol" Section</strong></p>
 
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/4/47/T--Berlin_diagnostX--Protocol_RNA_Iso_Seq.pdf">RNA Isolation and Sequencing</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/7/76/T--Berlin_diagnostX--12_Extension_PCR%2C_Gel_electrophoresis_%26_DNA_Clean_UP.pdf">Extension PCR, Electrophoresis and DNA Clean-Up</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/4/49/T--Berlin_diagnostX--NESTED.pdf">Nested PCR</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/a/a2/T--Berlin_diagnostX--Protocol_Colony_PCR.pdf">Colony PCR</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/6/6a/T--Berlin_diagnostX--Sensor_Screening.pdf">Toehold Switch Sensor – High troughput screening</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/3/37/T--Berlin_diagnostX--Protocol_Mutagenesis.pdf">Sensor Mutagenesis and Cloning</a></li>
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Latest revision as of 01:17, 16 December 2017

Experiments