Difference between revisions of "Team:Berlin diagnostX/Experiments"

 
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We approached these institutions by presenting on the one hand the global challenge of T.solium infection and on the other hand the opportunities that go along with the toehold switch technology. In this respect, we aimed to show potential sponsors and promoters how this technology could revolutionize field diagnostics – and to present the advantages of a team consisting of young, motivated scientists who are not yet primed to think inside their silos. <br> <br>
 
We approached these institutions by presenting on the one hand the global challenge of T.solium infection and on the other hand the opportunities that go along with the toehold switch technology. In this respect, we aimed to show potential sponsors and promoters how this technology could revolutionize field diagnostics – and to present the advantages of a team consisting of young, motivated scientists who are not yet primed to think inside their silos. <br> <br>
 
By this approach, we were able to convince different institutions to sponsor different aspects of our project. Whereas corporates of the biotechnological sector supported us with equipment, finance and consulting corporates were convinced of our team structure, and would support our participation in the iGEM competition. Institutional funding would often be given for international collaborations such as our project with India, or our participation in the Cystinet conference. </p>
 
By this approach, we were able to convince different institutions to sponsor different aspects of our project. Whereas corporates of the biotechnological sector supported us with equipment, finance and consulting corporates were convinced of our team structure, and would support our participation in the iGEM competition. Institutional funding would often be given for international collaborations such as our project with India, or our participation in the Cystinet conference. </p>
     <img class="img-fluid mt-5" src="https://static.igem.org/mediawiki/2017/9/96/T--Berlin_diagnostX--Cover_Kuchen.png">
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     <img class="img-fluid mt-5" src="https://static.igem.org/mediawiki/2017/1/1d/T--Berlin_diagnostX--Kuchen_neu.png">
 
      
 
      
 
     </div></div>
 
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             <li>Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li>
 
             <li>Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li>
 
         </ol></li>
 
         </ol></li>
      
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     <p class="mt-4"><strong>Please find detailed protocols under the "Protocol" Section</strong></p>
 
     </ul></div>
 
     </ul></div>
 
      
 
      
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             <h3 class="text-center igem_blue mt-4"><strong>Protocols</strong></h3>
 
             <h3 class="text-center igem_blue mt-4"><strong>Protocols</strong></h3>
 
         </div>
 
         </div>
    </div>
 
 
     </div>
 
     </div>
  
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     <div class="col-6">
 
     <div class="col-6">
 
     <ul>
 
     <ul>
         <li><a class="igem_blue" href="#transkriptom">Transcriptome analysis of T. solium</a>  
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         <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/4/47/T--Berlin_diagnostX--Protocol_RNA_Iso_Seq.pdf">RNA Isolation and Sequencing</a></li>
            <ol>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/7/76/T--Berlin_diagnostX--12_Extension_PCR%2C_Gel_electrophoresis_%26_DNA_Clean_UP.pdf">Extension PCR, Electrophoresis and DNA Clean-Up</a></li>
            <li>RNA Isolation</li>
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         <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/4/49/T--Berlin_diagnostX--NESTED.pdf">Nested PCR</a></li>
            <li>RNA sequencing</li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/a/a2/T--Berlin_diagnostX--Protocol_Colony_PCR.pdf">Colony PCR</a></li>
        </ol></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/6/6a/T--Berlin_diagnostX--Sensor_Screening.pdf">Toehold Switch Sensor – High troughput screening</a></li>
         <li><a class="igem_blue" href="#bioinformatics">Bioinformatics</a></li>
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        <li><a class="igem_blue" href="https://static.igem.org/mediawiki/2017/3/37/T--Berlin_diagnostX--Protocol_Mutagenesis.pdf">Sensor Mutagenesis and Cloning</a></li>
        <li><a class="igem_blue" href="#switch_synthese">Switch Synthesis</a>  
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            <ol>
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</ul>
            <li>Extension PCR</li>
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            <li>Nested PCR</li>
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            <li>Colony PCR</li>
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            <li>Gel Electrophoresis</li>
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            <li>DNA Clean-Up</li>
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            </ol></li></ul>
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     </div>
 
     </div>
     <div class="col-6">
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     <div class="col-6"></div>
        <ul>
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        <li><a class="igem_blue" href="#switch_testing">Toehold Switch Sensor Test – Testing Pipeline</a>
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            <ol>
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            <li>High troughput screening in liquid medium</li>
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            <li>Reliable cell free expression on cellulose membranes</li>
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        </ol></li>
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        <li><a class="igem_blue" href="#screening_opti">Optimizing the Screening Pipeline</a></li>
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        <li><a class="igem_blue" href="#andere_experimente">Other Experiments</a>
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            <ol>
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            <li>Preparation of nucleic acids (DNA preparation, Restriction digest/ DNA ligation, in vitro transcription, RNA purification)</li>
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            <li>Preparation of sensors for iGEM Submission (Mutagenesis & Cloning)</li>
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        </ol></li>
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        <p class="mt-4"><strong>Please find detailed protocols under the "Protocol" Section</strong></p>       
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    </ul></div>
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</div>
 
</div>
 
</div>   
 
</div>   
 
        
 
        
     
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</div>     
 
</body>
 
</body>
 
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Latest revision as of 01:17, 16 December 2017

Experiments