Difference between revisions of "Team:Kent/HP/Silver"
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<label class="hull-title" for="cb2">Professor Michelle Garrett</label> | <label class="hull-title" for="cb2">Professor Michelle Garrett</label> | ||
<label class="hull-close" for="acc-close"></label> | <label class="hull-close" for="acc-close"></label> | ||
| − | <div class="hull-content">When conceptualizing our project, we realized we would need to transfect our cell lines into mammalian cells if we wanted to have any use of it as a potential diagnostic tool, as it would be relatively useless in its bacterial cell host. Professor Garrett was able to guide us in the direction of the Smales Group at our University, providing us with the resources needed to make the transfections possible.</div> | + | <div class="hull-content">When conceptualizing our project, we realized we would need to transfect our cell lines into mammalian cells if we wanted to have any use of it as a potential diagnostic tool, as it would be relatively useless in its bacterial cell host. Professor Garrett was able to guide us in the direction of the Smales Group at our University, providing us with the resources needed to make the transfections possible.<br><img src="https://static.igem.org/mediawiki/2017/2/2b/T--Kent--HP8.png"></div> |
</section> | </section> | ||
<input type="radio" name="droptext" id="acc-close" /> | <input type="radio" name="droptext" id="acc-close" /> | ||
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<label class="hull-title" for="cb3">Ian Brown, Microscopy Suite Facility Manager</label> | <label class="hull-title" for="cb3">Ian Brown, Microscopy Suite Facility Manager</label> | ||
<label class="hull-close" for="acc-close"></label> | <label class="hull-close" for="acc-close"></label> | ||
| − | <div class="hull-content">As CRISPR itself has only recently been discovered as an mRNA tracking tool, most of the project entailed repeated trial and error. We realized before we even thought of imaging, we needed to ensure we had the appropriate controls for comparative and evaluative reasons. We were able to generate a repeatable layout for future imaging purposes that Ian Brown was able to confirm would be adequate in imaging our cells accurately | + | <div class="hull-content">As CRISPR itself has only recently been discovered as an mRNA tracking tool, most of the project entailed repeated trial and error. We realized before we even thought of imaging, we needed to ensure we had the appropriate controls for comparative and evaluative reasons. We were able to generate a repeatable layout for future imaging purposes that Ian Brown was able to confirm would be adequate in imaging our cells accurately<br> |
<img src="https://static.igem.org/mediawiki/2017/9/9d/T--Kent--HP5.png"></div> | <img src="https://static.igem.org/mediawiki/2017/9/9d/T--Kent--HP5.png"></div> | ||
</section> | </section> | ||
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<label class="hull-title" for="cb4">Dr. Emma Mead</label> | <label class="hull-title" for="cb4">Dr. Emma Mead</label> | ||
<label class="hull-close" for="acc-close"></label> | <label class="hull-close" for="acc-close"></label> | ||
| − | <div class="hull-content">Through research, we were able to conclude the best possible cells to transfect our constructs into would have to be HEK293 cells. However, without experience in such a field, we were guided to Dr. Mead, who proved to be invaluable throughout the entirety of our project. She confirmed that it would indeed have to be the HEK293 cells and provided us with samples to work with, as well as guiding us through the passaging and transfection itself. | + | <div class="hull-content">Through research, we were able to conclude the best possible cells to transfect our constructs into would have to be HEK293 cells. However, without experience in such a field, we were guided to Dr. Mead, who proved to be invaluable throughout the entirety of our project. She confirmed that it would indeed have to be the HEK293 cells and provided us with samples to work with, as well as guiding us through the passaging and transfection itself.<br> |
<img src="https://static.igem.org/mediawiki/2017/5/5a/T--Kent--HP6.png"></div> | <img src="https://static.igem.org/mediawiki/2017/5/5a/T--Kent--HP6.png"></div> | ||
</section> | </section> | ||
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<label class="hull-title" for="cb5">Dr. Rosalyn Masterton</label> | <label class="hull-title" for="cb5">Dr. Rosalyn Masterton</label> | ||
<label class="hull-close" for="acc-close"></label> | <label class="hull-close" for="acc-close"></label> | ||
| − | <div class="hull-content">We were able to conclude that we would need a vector to clone our construct before we could image it and decided upon pcDNA 3.1 (+) mammalian vector through the help of Dr. Masterton with her expertise in molecular biology. She also advised us who to speak to for the use of mammalian cell lines. | + | <div class="hull-content">We were able to conclude that we would need a vector to clone our construct before we could image it and decided upon pcDNA 3.1 (+) mammalian vector through the help of Dr. Masterton with her expertise in molecular biology. She also advised us who to speak to for the use of mammalian cell lines.<br> |
<img src="https://static.igem.org/mediawiki/2017/b/bf/T--Kent--HP7.png"></div> | <img src="https://static.igem.org/mediawiki/2017/b/bf/T--Kent--HP7.png"></div> | ||
</section> | </section> | ||
Revision as of 02:49, 16 December 2017
Integrated HP
