Difference between revisions of "Team:Kent/HP/Silver"

(Prototype team page)
 
 
(25 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
{{Kent}}
+
 
 +
 
 
<html>
 
<html>
−
<div class="column full_size judges-will-not-evaluate">
+
    <head>
−
<h3>★  ALERT! </h3>
+
        <link href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet" integrity="sha384-wvfXpqpZZVQGK6TAh5PVlGOfQNHSoD2xbE+QkPxCAFlNEevoEH3Sl0sibVcOQVnN" crossorigin="anonymous">
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
        <meta charset="utf-8">
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    </head>
−
</div>
+
    <style>
−
<div class="clear"></div>
+
        .logo_2017 a img{
 +
        display:none;
 +
        }
 +
        #firstHeading{
 +
        display:none;
 +
        }
 +
        #sideMenu{
 +
        display: none;
 +
        }
 +
        @font-face {
 +
        font-family: 'KentExo2';
 +
        src: url(https://static.igem.org/mediawiki/2017/7/76/T--Kent--Exo2.ttf) format('truetype');
 +
        }
 +
        #globalWrapper, #content { /*-- changes default wiki settings --*/
 +
        margin: 0px;
 +
        padding: 0px;
 +
        width: 100%;
 +
        height: 100%;
 +
        border: 0px;
 +
        background: #272e33 url("https://static.igem.org/mediawiki/2017/e/ee/T--Kent--Silverbkg.png") no-repeat center center fixed;
 +
        -webkit-background-size: cover;
 +
        -moz-background-size: cover;
 +
        -o-background-size: cover;
 +
        background-size: cover;
 +
        }
  
 +
        *{
 +
        padding: 0;
 +
        margin: 0;
 +
font-family: 'KentExo2';
 +
        }
  
−
<div class="column full_size">
+
        #navdiv{
 +
        color: #FFFFFF;
 +
        left:0px;
 +
        padding: 30px;
 +
        text-align: center;
 +
        font-family:KentExo2;
 +
        position:fixed;
 +
        top:10px;
 +
        z-index:100;
 +
        }
  
−
<h1>Silver Medal Human Practices</h1>
+
        #navdiv h1 img {
−
<p>iGEM teams are leading in the area of Human Practices because they conduct their projects within a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
+
        width:150px;
−
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
+
        }
−
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
+
−
</div>
+
  
−
<div class="clear"></div>
+
#navdiv h1 {
 +
line-height: 150px;
 +
margin-top:-25px;
 +
}
  
−
<div class="column half_size">
+
        #navdiv ul{
−
<h3>Silver Medal Criterion #3</h3>
+
background: transparent;
−
<p>Convince the judges you have thought carefully and creatively about whether your work is safe, responsible and good for the world. You could accomplish this through engaging with your local, national and/or international communities or other approaches. Please note that standard surveys will not fulfill this criteria.</p>
+
        text-align: center;
 +
        list-style: none;
 +
        padding: 0;
 +
        margin: 0;
 +
        display: block;
 +
                }
 +
 
 +
        #navdiv ul a{
 +
        text-decoration: none;
 +
        color: #FFFFFF;
 +
 
 +
        }
 +
        #navdiv ul li{
 +
margin-top: 30px;
 +
        float: left;
 +
        width: 169px;
 +
        height: 50px;
 +
        line-height: 50px;
 +
        position: relative;
 +
        text-transform: uppercase;
 +
        font-size: 16px;
 +
        color:#D0FEDC;
 +
        cursor: pointer;
 +
        opacity:0.8;
 +
border-radius: 10px;
 +
        }
 +
 
 +
#navdiv ul #teamLogo{
 +
margin-top:-10px;
 +
margin-left: 10px;
 +
margin-right: 10px;
 +
        float: left;
 +
        width: 150px;
 +
        height: 120px;
 +
        position: relative;
 +
        opacity:0.8;
 +
        }
 +
 
 +
        #navdiv ul li:hover{
 +
border-radius:10px;
 +
        background: #E61B5F;
 +
        transition:all 0.3s;
 +
        opacity:0.9;
 +
        }
 +
 
 +
 
 +
#navdiv ul #teamLogo:hover{
 +
border-radius:10px;
 +
        background: #455057;
 +
        transition:all 0.3s;
 +
        opacity:0.8;
 +
        }
 +
 
 +
 
 +
        #navdiv ul li:hover a{
 +
        color:#455057;
 +
        }
 +
 
 +
        #navdiv ul.drop-menu {
 +
        position: absolute;
 +
        top: 100%;
 +
        left: 0%;
 +
        width: 100%;
 +
        padding: 0;
 +
        z-index:100;
 +
        }
 +
 
 +
        #navdiv ul.drop-menu li {
 +
        background: #455057;
 +
        color: #FFFFFF;
 +
       
 +
        }
 +
        #navdiv ul.drop-menu li:hover {
 +
color:#455057;
 +
        background: #e2638e;
 +
 
 +
        }
 +
#navdiv ul.drop-menu li ul{
 +
        border-radius: 10px;
 +
        }
 +
     
 +
        #navdiv ul.drop-menu li {
 +
        display: none;
 +
        }
 +
 
 +
        #navdiv li:hover > ul.drop-menu{
 +
        perspective: 1000px;
 +
        }
 +
 
 +
        #navdiv li:hover > ul.drop-menu li {
 +
margin-top: 5px;
 +
        display: block;
 +
        text-align: center;
 +
        }
 +
 
 +
#title{
 +
margin-top:170px;
 +
}
 +
 
 +
        #title #header1{
 +
display:inline-block;
 +
        margin-left:8%;
 +
margin-right:10%;
 +
margin-bottom:100px;
 +
        width:230px;
 +
        }
 +
#title #header2{
 +
display:inline-block;
 +
        margin-left:10%;
 +
margin-right:3%;
 +
margin-bottom:100px;
 +
        width:300px;
 +
        }
 +
 
 +
#title span{
 +
position:absolute:
 +
margin-top:500px;
 +
display: inline-block;
 +
color:#E61B5F;
 +
font-size:50px;
 +
}
 +
 
 +
        .droptext {
 +
box-shadow: 0px 0px 0px 5px #E61B5F;
 +
border-radius:10px;
 +
        margin: auto;
 +
        margin-bottom:200px;
 +
        margin-top:0px;
 +
        width: 1200px;
 +
opacity:0.85;
 +
 
 +
}
 +
.droptext input {
 +
display: none;
 +
}
 +
.hull {
 +
position: relative;
 +
height: 100px;
 +
background: transparent;
 +
    transition: all .25s ease-in-out;
 +
}
 +
.hull::before {
 +
    content: '';
 +
    position: absolute;
 +
    display: block;
 +
    top: 0;
 +
    bottom: 0;
 +
    left: 0;
 +
    right: 0;
 +
    pointer-events: none;
 +
}
 +
header.hull {
 +
border-radius:10px 10px 0px 0px;
 +
border: 3px solid #455057;
 +
border-style:solid;
 +
border-bottom-color: #E61B5F;
 +
background: #455057;
 +
z-index: 50;
 +
cursor: initial;
 +
 
 +
}
 +
header .hull-title {
 +
margin: 0;
 +
font-weight: normal;
 +
font-size: 25pt;
 +
color: #E61B5F;
 +
cursor: initial;
 +
 
 +
}
 +
.hull-title {
 +
color:#efd0db;
 +
font-size: 20px;
 +
width: calc(100% - 40px);
 +
height: 50px;
 +
line-height: 50px;
 +
padding: 20px 0 0 50px;
 +
display: inline-block;
 +
cursor: pointer;
 +
-webkit-touch-callout: none;-webkit-user-select: none;-khtml-user-select: none;-moz-user-select: none;-ms-user-select: none;user-select: none;
 +
}
 +
.hull-content {
 +
width: calc(100% - 40px);
 +
padding: 30px 0 20px 20px;
 +
font-size: 11pt;
 +
color: #455057;
 +
display: none;
 +
}
 +
.hull-close {
 +
position: absolute;
 +
height: 64px;
 +
width: 100%;
 +
top: 0;
 +
left: 0;
 +
cursor: pointer;
 +
display: none;
 +
}
 +
input:checked + .hull {
 +
height: auto;
 +
color:#455057;
 +
background:#efd0db;
 +
border-top: 2.5px solid #E61B5F;
 +
 
 +
}
 +
input:checked + .hull .hull-title {
 +
color:#455057;
 +
}
 +
 
 +
input:checked + .hull .hull-content,
 +
input:checked + .hull .hull-close {
 +
display: inline-block;
 +
border-bottom: 2.5px solid #E61B5F;
 +
}
 +
 
 +
.arrows section .hull-title {
 +
padding-left: 44px;
 +
width: calc(100% - 80px);
 +
}
 +
input:checked + .arrows section .hull-title{
 +
color:#455057;
 +
}
 +
 
 +
.arrows section .hull-title:before {
 +
position: absolute;
 +
display: block;
 +
content: '\203a';
 +
font-size: 28pt;
 +
left: 20px;
 +
top: 17px;
 +
color: #E61B5F;
 +
transition: transform .5s ease-in-out;
 +
 +
}
 +
input:checked + section.hull .hull-title:before {
 +
transform: rotate(270deg);
 +
 
 +
}
 +
 
 +
 
 +
#box1{
 +
margin:auto;
 +
width:1200px;
 +
text-align:center;
 +
}
 +
 
 +
#box2{
 +
margin:auto;
 +
width:1200px;
 +
text-align:center;
 +
 
 +
 
 +
}
 +
#textbox1{
 +
display:inline-block;
 +
margin: 40px 10px 40px 0;
 +
width:500px;
 +
border-radius:20px;
 +
opacity:0.8;
 +
background: #455057;
 +
border-style: solid;
 +
border-color: #E61B5F;
 +
text-align:center;
 +
}
 +
#textbox3{
 +
display:inline-block;
 +
margin: 40px 0 40px 0;
 +
width:900px;
 +
border-radius:20px;
 +
opacity:0.8;
 +
background: #455057;
 +
border-style: solid;
 +
border-color: #E61B5F;
 +
text-align:center;
 +
}
 +
#textbox4{
 +
display:inline-block;
 +
margin: 40px 0 40px 0;
 +
width:800px;
 +
border-radius:20px;
 +
opacity:0.8;
 +
background: #455057;
 +
border-style: solid;
 +
border-color: #E61B5F;
 +
text-align:center;
 +
}
 +
 
 +
#textbox2{
 +
display:inline-block;
 +
margin: 0px 0 0px 0;
 +
width:1000px;
 +
border-radius:20px;
 +
 
 +
opacity:0.8;
 +
background: #455057;
 +
border-style: solid;
 +
border-color: #E61B5F;
 +
text-align:center;
 +
}
 +
 
 +
}
 +
.connector{
 +
text-align:center;
 +
}
 +
.connector img{
 +
display:inline-block;
 +
width:50px;
 +
}
 +
 
 +
#box1 p{
 +
display:inline-block;
 +
width: calc(100% - 40px);
 +
font-size:20px;
 +
color:#E61B5F;
 +
}
 +
#box2 p{
 +
display:inline-block;
 +
width: calc(100% - 40px);
 +
font-size:20px;
 +
color:#E61B5F;
 +
}
 +
 
 +
#title2{
 +
padding: 50px 0 50px 0;
 +
}
 +
.centerizer{
 +
width:100%;
 +
text-align:center;
 +
margin: 0 0 0 0;
 +
}
 +
.centerizer span{
 +
color:#f4ccb2;
 +
font-size:50px;
 +
}
 +
        #foot ul{
 +
        display: flex;
 +
        position: fixed;
 +
        top:92%;
 +
        left:50%;
 +
        transform: translate(-50%,-50%);
 +
        }
 +
        #foot ul li{
 +
        list-style: none;
 +
        }
 +
        #foot ul li span{
 +
        width:50px;
 +
        height:50px;
 +
        background: #455057;
 +
        text-align:center;
 +
        line-height:50px;
 +
        font-size: 25px;
 +
        margin: 0 15px;
 +
        display: block;
 +
        border-radius: 50%;
 +
        position: relative;
 +
        overflow:hidden;
 +
        border: 4px solid #455057;
 +
        z-index:50;
 +
        }
 +
        #foot ul li span .fa{
 +
        margin:25%;
 +
        position:relative;
 +
        color:white;
 +
        transition: 0.5s;
 +
}
 +
 
 +
        #foot ul li span:hover .fa
 +
        {
 +
        color:#25343D;
 +
        transform: rotateY(360deg);
 +
        }
 +
        #foot ul li span:before
 +
        {
 +
        content:'';
 +
        position: absolute;
 +
        top:100%;
 +
        left:0;
 +
        width:100%;
 +
        height:100%;
 +
        background: #1AE195;
 +
        transition: 0.5s;
 +
        }
 +
        #foot ul li span:hover:before{
 +
        top:0;
 +
        }
 +
        #foot ul li:nth-child(1) span:before{
 +
        background: #E10C2B;
 +
        }
 +
        #foot ul li:nth-child(2) span:before{
 +
        background: #AA5EE7;
 +
        }
 +
        #foot ul li:nth-child(3) span:before{
 +
        background: #09F99E;
 +
        }
 +
        #foot ul li:nth-child(4) span:before{
 +
        background: #0367A6;
 +
        }
 +
        #foot ul li:nth-child(5) span:before{
 +
        background: #299CE4;
 +
        }
 +
        #foot ul li:nth-child(6) span:before{
 +
        background: #5BBBF7;
 +
        }
 +
 
 +
#sponsors{
 +
margin-left:50px;
 +
}
 +
#sponsors img{
 +
width:150px;
 +
}
 +
 
 +
#sponsorsdown #snapg img{
 +
margin-left:750px;
 +
}
 +
 
 +
    </style>
 +
 
 +
 
 +
 
 +
    <body>
 +
        <nav id="navdiv">
 +
            <ul>
 +
                <li>
 +
                    <a href="#">Project</a>
 +
                    <ul class="drop-menu menu-1">
 +
                        <a href="https://2017.igem.org/Team:Kent/Description"><li>Description</li></a>
 +
                      <a href="https://2017.igem.org/Team:Kent/Results"><li>Results</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Model"><li>Modelling</li></a>
 +
 
 +
                    </ul>
 +
                <li>
 +
                    <a href="#">Parts</a>
 +
                    <ul class="drop-menu menu-2">
 +
                        <a href="https://2017.igem.org/Team:Kent/Basic_Part"><li>Basic Parts</li></a>
 +
 
 +
 
 +
                    </ul>
 +
                </li>
 +
                <li>
 +
                    <a href="#">Notebook</a>
 +
                    <ul class="drop-menu menu-2">
 +
                        <a href="https://2017.igem.org/Team:Kent/Notebook"><li>Logbook</li></a>
 +
<a href="https://2017.igem.org/Team:Kent/Experiments"><li>Experiments</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Improve"><li>Future Ideas</li></a>
 +
                       
 +
                    </ul>
 +
                </li>
 +
                <li id="teamLogo">
 +
                    <a href="https://2017.igem.org/Team:Kent">
 +
                        <h1><img src="https://static.igem.org/mediawiki/2017/thumb/4/4f/T--Kent--Kenttemplogo.png/667px-T--Kent--Kenttemplogo.png"></h1>
 +
                    </a>
 +
                </li>
 +
                <li>
 +
                    <a href="#">Safety</a>
 +
                    <ul class="drop-menu menu-2">
 +
                        <a href="https://2017.igem.org/Team:Kent/Safety"><li>Project Safety</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Signs"><li>Hazard Signs</li></a>
 +
                    </ul>
 +
                </li>
 +
                <li>
 +
                    <a href="#">Team</a>
 +
                    <ul class="drop-menu menu-2">
 +
                        <a href="https://2017.igem.org/Team:Kent/Team"><li>Meet the Team</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Contribution"><li>Contribution</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Attributions"><li>Attribution</li></a>
 +
                    </ul>
 +
                </li>
 +
                <li>
 +
                    <a href="#">Human Practices</a>
 +
                    <ul class="drop-menu menu-2">
 +
                        <a href="https://2017.igem.org/Team:Kent/HP/Silver"><li>Integrated HP</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Engagement"><li>Public Engagement</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/InterLab"><li>Interlab</li></a>
 +
                        <a href="https://2017.igem.org/Team:Kent/Collaborations"><li>Collaboration</li></a>
 +
                    </ul>
 +
                </li>
 +
            </ul>
 +
        </nav>
 +
        <div id ="title">
 +
                <img src = "https://static.igem.org/mediawiki/2017/thumb/4/46/T--Kent--SilverHeader.png/401px-T--Kent--SilverHeader.png" id="header1">
 +
<span>Integrated HP</span>
 +
<img src = "https://static.igem.org/mediawiki/2017/thumb/1/12/T--Kent--SilverHeader2.png/766px-T--Kent--SilverHeader2.png" id="header2">
 +
        </div>
 +
       
 +
<div id="box1">
 +
 
 +
 
 +
<div id="textbox2" >
 +
<p>Our team has strived to make human practices the solid foundation of our project right from the start. We wanted to ensure we fully understood the implications of any project we chose to pursue while addressing current global problems.
 +
<br>
 +
We held a brainstorming session where we bounced ideas off of one another for potential project ideas and had narrowed it down to a top five. We decided we needed to consult with our immediate community to scope out what issues they deemed important that needed immediate addressing. Through different outreach opportunities, we were able to comprehend the controversy surrounding GMOs and genetic engineering in general. This caused us to critically analyze our project ideas before deciding upon ‘LuCAS’: a novel way of mRNA localization using CRISPR - dCas13a.
 +
</p>
 +
</div>
 
</div>
 
</div>
  
−
<div class="column half_size">
+
<div class="centerizer"  id="title2">
−
<h5>Some Human Practices topic areas </h5>
+
<span>Why CRISPR-dCas13a (C2C2)?</span>
−
<ul>
+
<div class="SlineSeparator"></div>
−
<li>Philosophy</li>
+
−
<li>Public Engagement / Dialogue</li>
+
−
<li>Education</li>
+
−
<li>Product Design</li>
+
−
<li>Scale-Up and Deployment Issues</li>
+
−
<li>Environmental Impact</li>
+
−
<li>Ethics</li>
+
−
<li>Safety</li>
+
−
<li>Security</li>
+
−
<li>Public Policy</li>
+
−
<li>Law and Regulation</li>
+
−
<li>Risk Assessment</li>
+
−
</ul>
+
 
</div>
 
</div>
  
 +
<div id="box2">
  
−
<div class="column half_size">
+
<div id="textbox2" >
−
<h5>What should we write about on this page?</h5>
+
<p>We were introduced to CRISPR-Cas9 as a tool for targeting cellular mRNA translation through Dr. Peter Ellis, a lecturer in Molecular Biology and Reproduction. However, through research, we discovered that Cas9 is a DNA endonuclease, meaning it recognizes DNA targets and cuts them. It would need to be ‘tricked’ by molecular means. C2C2, however, or Cas13a is an RNA endonuclease, meaning it cuts the RNA rather than the DNA. There are two key differences when comparing Cas13 to CAS9: Firstly, Cas13 recognizes RNA rather than DNA. Secondly, once the target is recognized, it acts promiscuously and starts to ‘chew up’ all of the RNA around it, not only the intended target sequence. <br><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Kent--LogbookP5.png"><img src="https://static.igem.org/mediawiki/2017/5/52/T--Kent--LogbookP3.png">
−
<p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement). This should include all of the work done for the Silver Medal Criterion #3. Details for your Gold medal work and/or work for the two Human Practices special prizes should be put on those specified pages.</p>
+
</p>
 
</div>
 
</div>
 +
<div class="centerizer">
 +
<div class="connector">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/f/f4/T--Kent--HP9.png/133px-T--Kent--HP9.png">
 +
</div>
 +
<div id="textbox2" >
 +
<p>
 +
We wanted to utilize this knowledge and build upon the CRISPR-Cas9 and produce a tool that would just track the mRNA, not cut it. We did not want to overcomplicate our methodology and deemed tagging the mRNA with GFP would be sufficient as we would be able to essentially track the mRNA movement to ensure its localization without damaging its integrity through cutting it.
 +
</p>
 +
</div>
 +
</div>
 +
<div class="centerizer">
 +
<div class="connector">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/f/f4/T--Kent--HP9.png/133px-T--Kent--HP9.png">
 +
</div>
 +
</div>
 +
<nav class="droptext arrows">
 +
<header class="hull">
 +
<label for="acc-close" class="hull-title">Surveys and Consultations</label>
 +
</header>
 +
<input type="radio" name="droptext" id="cb1" />
 +
<section class="hull">
 +
<label class="hull-title" for="cb1">Surveys</label>
 +
<label class="hull-close" for="acc-close"></label>
 +
<div class="hull-content"><ul><li>We wanted the opportunity to receive insight from the public immediately, even before deciding on a project idea. We wanted to ensure that any topic we would be putting our focus into would heed positive results, not only from the scientific community but from the general public as well.</li>
  
 +
<li>We set out to design several surveys, questioning the community at our University and locally on their knowledge of GMOs and whether our project abstract was an idea they agreed would be beneficial once produced. </li>
  
−
<div class="column half_size">
+
<li>The general consensus from the students at the University was that they agreed genetic modification would be beneficial when used appropriately, however strict regulations would need to be in place when dealing with GMO applications, a small percentage even saying they would go the extra mile to ensure they did not deal with GMOs in their day to day lives.</li>
−
<h5>Inspiration</h5>
+
<li><img src="https://static.igem.org/mediawiki/2017/e/e8/T--Kent--HP1.png"></li>
−
<p>Read what other teams have done:</p>
+
<li>With this in mind, we introduced our project abstract and explained the positive outcomes we saw it producing and its potential as a diagnostic tool. The majority of the feedback we received was positive and allowed us to confidently advance in our laboratory work with a goal in mind: produce a tool with a promising future in diagnositcs.
−
<ul>
+
</li>
−
<li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
+
<li><img src="https://static.igem.org/mediawiki/2017/d/d9/T--Kent--HP2.png"><img src="https://static.igem.org/mediawiki/2017/6/60/T--Kent--HP3.png"></li>
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Policy_Practices_Overview">2014 UC Davis </a></li>
+
<li><img src="https://static.igem.org/mediawiki/2017/8/84/T--Kent--HP4.png"></li>
−
<li><a href="https://2013.igem.org/Team:Manchester/HumanPractices">2013 Manchester </a></li>
+
−
<li><a href="https://2013.igem.org/Team:Cornell/outreach">2013 Cornell </a></li>
+
−
</ul>
+
 
</div>
 
</div>
 +
</section>
 +
<input type="radio" name="droptext" id="acc-close" />
 +
<input type="radio" name="droptext" id="cb2" />
 +
<section class="hull">
 +
<label class="hull-title" for="cb2">Professor Michelle Garrett</label>
 +
<label class="hull-close" for="acc-close"></label>
 +
<div class="hull-content">When conceptualizing our project, we realized we would need to transfect our cell lines into mammalian cells if we wanted to have any use of it as a potential diagnostic tool, as it would be relatively useless in its bacterial cell host. Professor Garrett was able to guide us in the direction of the Smales Group at our University, providing us with the resources needed to make the transfections possible.<br><img src="https://static.igem.org/mediawiki/2017/2/2b/T--Kent--HP8.png"></div>
 +
</section>
 +
<input type="radio" name="droptext" id="acc-close" />
 +
<input type="radio" name="droptext" id="cb3" />
 +
<section class="hull">
 +
<label class="hull-title" for="cb3">Ian Brown, Microscopy Suite Facility Manager</label>
 +
<label class="hull-close" for="acc-close"></label>
 +
<div class="hull-content">As CRISPR itself has only recently been discovered as an mRNA tracking tool, most of the project entailed repeated trial and error. We realized before we even thought of imaging, we needed to ensure we had the appropriate controls for comparative and evaluative reasons. We were able to generate a repeatable layout for future imaging purposes that Ian Brown was able to confirm would be adequate in imaging our cells accurately<br>
 +
<img src="https://static.igem.org/mediawiki/2017/9/9d/T--Kent--HP5.png"></div>
 +
</section>
 +
<input type="radio" name="droptext" id="acc-close" />
 +
<input type="radio" name="droptext" id="cb4" />
 +
<section class="hull">
 +
<label class="hull-title" for="cb4">Dr. Emma Mead</label>
 +
<label class="hull-close" for="acc-close"></label>
 +
<div class="hull-content">Through research, we were able to conclude the best possible cells to transfect our constructs into would have to be HEK293 cells. However, without experience in such a field, we were guided to Dr. Mead, who proved to be invaluable throughout the entirety of our project. She confirmed that it would indeed have to be the HEK293 cells and provided us with samples to work with, as well as guiding us through the passaging and transfection itself.<br>
 +
<img src="https://static.igem.org/mediawiki/2017/5/5a/T--Kent--HP6.png"></div>
 +
</section>
 +
<input type="radio" name="droptext" id="acc-close" />
 +
<input type="radio" name="droptext" id="cb5" />
 +
<section class="hull">
 +
<label class="hull-title" for="cb5">Dr. Rosalyn Masterton</label>
 +
<label class="hull-close" for="acc-close"></label>
 +
<div class="hull-content">We were able to conclude that we would need a vector to clone our construct before we could image it and decided upon pcDNA 3.1 (+) mammalian vector through the help of Dr. Masterton with her expertise in molecular biology. She also advised us who to speak to for the use of mammalian cell lines.<br>
 +
<img src="https://static.igem.org/mediawiki/2017/b/bf/T--Kent--HP7.png"></div>
 +
</section>
 +
<input type="radio" name="droptext" id="acc-close" />
  
 +
</nav>
  
 +
        <div id="foot">
 +
            <ul>
 +
                <li><span><a href="https://www.youtube.com/channel/UCV4kIcIodwiGjgEX_Rad1Ig"><i class="fa fa-youtube" aria-hidden="true"></i></a></span></li>
 +
                <li><span><a href="https://www.instagram.com/igem_kent2017/?hl=en"><i class="fa fa-instagram" aria-hidden="true"></i></a></span></li>
 +
                <li><span><a href="#"><i class="fa fa-envelope" aria-hidden="true"></i></a></span></li>
 +
                <li><span><a href="https://www.facebook.com/iGEMkent2017"><i class="fa fa-facebook-square" aria-hidden="true"></i></a></span></li>
 +
                <li><span><a href="https://www.linkedin.com/company/18159542/"><i class="fa fa-linkedin" aria-hidden="true"></i></a></span></li>
 +
                <li><span><a href="https://twitter.com/Kent_iGEM"><i class="fa fa-twitter" aria-hidden="true"></i></a></span></li>
 +
            </ul>
 +
        </div>
 +
<div id="sponsors">
 +
<div id="sponsorstop">
 +
<a href="https://www.kent.ac.uk/" id="ukc">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/a/a3/T--Kent--UKCwhite.png/800px-T--Kent--UKCwhite.png">
 +
</a>
 +
<a href="http://avokiddo.com/" id="avo">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/a/a7/T--Kent--avokiddo.png/800px-T--Kent--avokiddo.png">
 +
</a>
 +
</div>
  
 +
<div id ="sponsorsdown">
 +
<a href="http://www.clicktravel.com" id="clickt">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/1/1e/T--Kent--clicktravel.png/799px-T--Kent--clicktravel.png">
 +
</a>
 +
<a href="http://www.bbsrc.ac.uk/" id="bbsrc">
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/4/4e/T--Kent--bbsrc.png/800px-T--Kent--bbsrc.png">
 +
</a>
 +
<a href="http://www.snapgene.com/" id="snapg">
 +
<img src="https://static.igem.org/mediawiki/2017/3/36/T--Kent--snapgene.png">
 +
</a>
 +
</div>
 +
</div>
 +
    </body>
 
</html>
 
</html>

Latest revision as of 03:03, 16 December 2017


Integrated HP

Our team has strived to make human practices the solid foundation of our project right from the start. We wanted to ensure we fully understood the implications of any project we chose to pursue while addressing current global problems.
We held a brainstorming session where we bounced ideas off of one another for potential project ideas and had narrowed it down to a top five. We decided we needed to consult with our immediate community to scope out what issues they deemed important that needed immediate addressing. Through different outreach opportunities, we were able to comprehend the controversy surrounding GMOs and genetic engineering in general. This caused us to critically analyze our project ideas before deciding upon ‘LuCAS’: a novel way of mRNA localization using CRISPR - dCas13a.

Why CRISPR-dCas13a (C2C2)?

We were introduced to CRISPR-Cas9 as a tool for targeting cellular mRNA translation through Dr. Peter Ellis, a lecturer in Molecular Biology and Reproduction. However, through research, we discovered that Cas9 is a DNA endonuclease, meaning it recognizes DNA targets and cuts them. It would need to be ‘tricked’ by molecular means. C2C2, however, or Cas13a is an RNA endonuclease, meaning it cuts the RNA rather than the DNA. There are two key differences when comparing Cas13 to CAS9: Firstly, Cas13 recognizes RNA rather than DNA. Secondly, once the target is recognized, it acts promiscuously and starts to ‘chew up’ all of the RNA around it, not only the intended target sequence.

We wanted to utilize this knowledge and build upon the CRISPR-Cas9 and produce a tool that would just track the mRNA, not cut it. We did not want to overcomplicate our methodology and deemed tagging the mRNA with GFP would be sufficient as we would be able to essentially track the mRNA movement to ensure its localization without damaging its integrity through cutting it.