Difference between revisions of "Team:HUST-China/Basic part"

 
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                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336026">BBa_K2336026</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336026">BBa_K2336026</a></td>
−
                                 <td>OprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1</td>
+
                                 <td>oprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT1,a lanthanide-binding tag to recycle ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT1, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>
 
                               </tr>
  
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336027">BBa_K2336027</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336027">BBa_K2336027</a></td>
−
                                 <td>OprF-GS-FLAG-LBT3-GS-LBT3-GS-LBT3</td>
+
                                 <td>oprF-GS-FLAG-LBT3-GS-LBT3-GS-LBT3</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT3,a lanthanide-binding tag to recycle ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT3, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
  
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336028">BBa_K2336028</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336028">BBa_K2336028</a></td>
−
                                 <td>OprF-GS-FLAG-LBT4-GS-LBT4-GS-LBT4</td>
+
                                 <td>oprF-GS-FLAG-LBT4-GS-LBT4-GS-LBT4</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT4,a lanthanide-binding tag to recycle ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT4, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
  
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336029">BBa_K2336029</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336029">BBa_K2336029</a></td>
−
                                 <td>OprF-GS-FLAG-LBT6-GS-LBT6</td>
+
                                 <td>oprF-GS-FLAG-LBT6-GS-LBT6-GS-LBT6</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT6, a lanthanide-binding tag to recycle ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT6, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
  
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336030">BBa_K2336030</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336030">BBa_K2336030</a></td>
−
                                 <td>OprF-GS-FLAG-LBT7-GS-LBT7-GS-LBT7</td>
+
                                 <td>oprF-GS-FLAG-LBT7-GS-LBT7-GS-LBT7</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT7,a lanthanide-binding tag to recycle lanthanide ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT7, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>  
 
                               </tr>  
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336031">BBa_K2336031</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336031">BBa_K2336031</a></td>
−
                                 <td>OprF-GS-FLAG-LBT8-GS-LBT8-GS-LBT8</td>
+
                                 <td>oprF-GS-FLAG-LBT8-GS-LBT8-GS-LBT8</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT8,a lanthanide-binding tag to recycle lanthanide ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT8, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336032">BBa_K2336032</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336032">BBa_K2336032</a></td>
−
                                 <td>OprF-GS-FLAG-LBT10-GS-LBT10-GS-LBT10</td>
+
                                 <td>oprF-GS-FLAG-LBT10-GS-LBT10-GS-LBT10</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT10,a lanthanide-binding tag to recycle lanthanide ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT10, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336033">BBa_K2336033</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336033">BBa_K2336033</a></td>
−
                                 <td>OprF-GS-FLAG-LBT11-GS-LBT11</td>
+
                                 <td>oprF-GS-FLAG-LBT11-GS-LBT11-GS-LBT11</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT11,a lanthanide-binding tag to recycle lanthanide ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT11, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>
 
                               </tr>
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336034">BBa_K2336034</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336034">BBa_K2336034</a></td>
−
                                 <td>OprF-GS-FLAG-LBT12-GS-LBT12-GS-LBT12</td>
+
                                 <td>oprF-GS-FLAG-LBT12-GS-LBT12-GS-LBT12</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables LBT12,a lanthanide-binding tag to recycle lanthanide ions.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT12, a lanthanide-binding tag can capture lanthanide ions around the bacteria.</td>
 
                               </tr>   
 
                               </tr>   
 
                               <tr>
 
                               <tr>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336035">BBa_K2336035</a></td>
 
                                 <td><a href="http://parts.igem.org/Part:BBa_K2336035">BBa_K2336035</a></td>
−
                                 <td>OprF-GS-Sitag-FLAG</td>
+
                                 <td>oprF-GS-Sitag-FLAG</td>
−
                                 <td>The OprF protein can act as an anchoring protein on E.coli’s surface, which enables us to recycle bacteria by silicon surface.</td>
+
                                 <td>The OprF protein can act as an anchored protein on E.coli’s surface, with which, Sitag can help the bacteria be recycled by binding with a silicon board.</td>
 
                               </tr>   
 
                               </tr>   
 
                               <tr>
 
                               <tr>
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                     </div>
 
                     </div>
 
                     <div class="col-xs-12">
 
                     <div class="col-xs-12">
−
                         <p>Those binding tag are highly effective and specific (see part page for experiment result). Different type of them allow user to custom their own combination.</p>
+
                         <p>Those binding tags are highly effective and specific (see part page for experiment result). Different types of them allow users to customize their own combination.</p>
 +
                        <p>We have improved our Sitag-part. Two years ago, we used it in yeast. Now we put gene into E.coli, while we have optimized its codons. Next we will do experiment to test its binding ability. We hope it will have the effect as we expected.</p>
 
                     </div>
 
                     </div>
 
                 </div>         
 
                 </div>         

Latest revision as of 03:27, 16 December 2017

Basic part

「Basic part」

Click Part Number to see more details:

Part Number Name Description
BBa_K2336026 oprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT1, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336027 oprF-GS-FLAG-LBT3-GS-LBT3-GS-LBT3 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT3, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336028 oprF-GS-FLAG-LBT4-GS-LBT4-GS-LBT4 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT4, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336029 oprF-GS-FLAG-LBT6-GS-LBT6-GS-LBT6 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT6, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336030 oprF-GS-FLAG-LBT7-GS-LBT7-GS-LBT7 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT7, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336031 oprF-GS-FLAG-LBT8-GS-LBT8-GS-LBT8 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT8, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336032 oprF-GS-FLAG-LBT10-GS-LBT10-GS-LBT10 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT10, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336033 oprF-GS-FLAG-LBT11-GS-LBT11-GS-LBT11 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT11, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336034 oprF-GS-FLAG-LBT12-GS-LBT12-GS-LBT12 The OprF protein can act as an anchored protein on E.coli’s surface, with which, LBT12, a lanthanide-binding tag can capture lanthanide ions around the bacteria.
BBa_K2336035 oprF-GS-Sitag-FLAG The OprF protein can act as an anchored protein on E.coli’s surface, with which, Sitag can help the bacteria be recycled by binding with a silicon board.
BBa_K2336036 PmrC-GFP PmrC promoter can be activated by phosphorylated PmrA.

Those binding tags are highly effective and specific (see part page for experiment result). Different types of them allow users to customize their own combination.

We have improved our Sitag-part. Two years ago, we used it in yeast. Now we put gene into E.coli, while we have optimized its codons. Next we will do experiment to test its binding ability. We hope it will have the effect as we expected.

Acknowledgments: