Difference between revisions of "Team:Queens Canada/Protocols"
| Line 2: | Line 2: | ||
{{Team:Queens_Canada/QU17_Menu}} | {{Team:Queens_Canada/QU17_Menu}} | ||
{{Team:Queens_Canada/QU17_Style}} | {{Team:Queens_Canada/QU17_Style}} | ||
| + | {{Team:Queens_Canada/Resources/CSS}} | ||
<html> | <html> | ||
<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
<link rel="stylesheet" href="https://www.w3schools.com/w3css/4/w3.css"> | <link rel="stylesheet" href="https://www.w3schools.com/w3css/4/w3.css"> | ||
<head> | <head> | ||
| − | |||
<script> | <script> | ||
function myFunction(id) { | function myFunction(id) { | ||
Revision as of 15:24, 18 August 2017
/*! Pure v0.5.0 Copyright 2014 Yahoo! Inc. All rights reserved. Licensed under the BSD License. https://github.com/yui/pure/blob/master/LICENSE.md
- /
/*! normalize.css v1.1.3 | MIT License | git.io/normalize Copyright (c) Nicolas Gallagher and Jonathan Neal
- /
/*! normalize.css v1.1.3 | MIT License | git.io/normalize */
/* ==========================================================================
HTML5 display definitions ========================================================================== */
/**
* Correct `block` display not defined in IE 6/7/8/9 and Firefox 3. */
article, aside, details, figcaption, figure, footer, header, hgroup, main, nav, section, summary {
display: block;
}
/**
* Correct `inline-block` display not defined in IE 6/7/8/9 and Firefox 3. */
audio, canvas, video {
display: inline-block; *display: inline; *zoom: 1;
}
/**
* Prevent modern browsers from displaying `audio` without controls. * Remove excess height in iOS 5 devices. */
audio:not([controls]) {
display: none; height: 0;
}
/**
* Address styling not present in IE 7/8/9, Firefox 3, and Safari 4. * Known issue: no IE 6 support. */
[hidden] {
display: none;
}
/* ==========================================================================
Base ========================================================================== */
/**
* 1. Correct text resizing oddly in IE 6/7 when body `font-size` is set using * `em` units. * 2. Prevent iOS text size adjust after orientation change, without disabling * user zoom. */
html {
font-size: 100%; /* 1 */ -ms-text-size-adjust: 100%; /* 2 */ -webkit-text-size-adjust: 100%; /* 2 */
}
/**
* Address `font-family` inconsistency between `textarea` and other form * elements. */
html, button, input, select, textarea {
font-family: sans-serif;
}
/**
* Address margins handled incorrectly in IE 6/7. */
body {
margin: 0;
}
/* ==========================================================================
Links ========================================================================== */
/**
* Address `outline` inconsistency between Chrome and other browsers. */
a:focus {
outline: thin dotted;
}
/**
* Improve readability when focused and also mouse hovered in all browsers. */
a:active, a:hover {
outline: 0;
}
/* ==========================================================================
Typography ========================================================================== */
/**
* Address font sizes and margins set differently in IE 6/7. * Address font sizes within `section` and `article` in Firefox 4+, Safari 5, * and Chrome. */
h1 {
font-size: 2em; margin: 0.67em 0;
}
h2 {
font-size: 1.5em; margin: 0.83em 0;
}
h3 {
font-size: 1.17em; margin: 1em 0;
}
h4 {
font-size: 1em; margin: 1.33em 0;
}
h5 {
font-size: 0.83em; margin: 1.67em 0;
}
h6 {
font-size: 0.67em; margin: 2.33em 0;
}
/**
* Address styling not present in IE 7/8/9, Safari 5, and Chrome. */
abbr[title] {
border-bottom: 1px dotted;
}
/**
* Address style set to `bolder` in Firefox 3+, Safari 4/5, and Chrome. */
b, strong {
font-weight: bold;
}
blockquote {
margin: 1em 40px;
}
/**
* Address styling not present in Safari 5 and Chrome. */
dfn {
font-style: italic;
}
/**
* Address differences between Firefox and other browsers. * Known issue: no IE 6/7 normalization. */
hr {
-moz-box-sizing: content-box; box-sizing: content-box; height: 0;
}
/**
* Address styling not present in IE 6/7/8/9. */
mark {
background: #ff0; color: #000;
}
/**
* Address margins set differently in IE 6/7. */
p, pre {
margin: 1em 0;
}
/**
* Correct font family set oddly in IE 6, Safari 4/5, and Chrome. */
code, kbd, pre, samp {
font-family: monospace, serif; _font-family: 'courier new', monospace; font-size: 1em;
}
/**
* Improve readability of pre-formatted text in all browsers. */
pre {
white-space: pre; white-space: pre-wrap; word-wrap: break-word;
}
/**
* Address CSS quotes not supported in IE 6/7. */
q {
quotes: none;
}
/**
* Address `quotes` property not supported in Safari 4. */
q:before, q:after {
content: ; content: none;
}
/**
* Address inconsistent and variable font size in all browsers. */
small {
font-size: 80%;
}
/**
* Prevent `sub` and `sup` affecting `line-height` in all browsers. */
sub, sup {
font-size: 75%; line-height: 0; position: relative; vertical-align: baseline;
}
sup {
top: -0.5em;
}
sub {
bottom: -0.25em;
}
/* ==========================================================================
Lists ========================================================================== */
/**
* Address margins set differently in IE 6/7. */
dl, menu, ol, ul {
margin: 1em 0;
}
dd {
margin: 0 0 0 40px;
}
/**
* Address paddings set differently in IE 6/7. */
menu, ol, ul {
padding: 0 0 0 40px;
}
/**
* Correct list images handled incorrectly in IE 7. */
nav ul, nav ol {
list-style: none; list-style-image: none;
}
/* ==========================================================================
Embedded content ========================================================================== */
/**
* 1. Remove border when inside `a` element in IE 6/7/8/9 and Firefox 3. * 2. Improve image quality when scaled in IE 7. */
img {
border: 0; /* 1 */ -ms-interpolation-mode: bicubic; /* 2 */
}
/**
* Correct overflow displayed oddly in IE 9. */
svg:not(:root) {
overflow: hidden;
}
/* ==========================================================================
Figures ========================================================================== */
/**
* Address margin not present in IE 6/7/8/9, Safari 5, and Opera 11. */
figure {
margin: 0;
}
/* ==========================================================================
Forms ========================================================================== */
/**
* Correct margin displayed oddly in IE 6/7. */
form {
margin: 0;
}
/**
* Define consistent border, margin, and padding. */
fieldset {
border: 1px solid #c0c0c0; margin: 0 2px; padding: 0.35em 0.625em 0.75em;
}
/**
* 1. Correct color not being inherited in IE 6/7/8/9. * 2. Correct text not wrapping in Firefox 3. * 3. Correct alignment displayed oddly in IE 6/7. */
legend {
border: 0; /* 1 */ padding: 0; white-space: normal; /* 2 */ *margin-left: -7px; /* 3 */
}
/**
* 1. Correct font size not being inherited in all browsers. * 2. Address margins set differently in IE 6/7, Firefox 3+, Safari 5, * and Chrome. * 3. Improve appearance and consistency in all browsers. */
button, input, select, textarea {
font-size: 100%; /* 1 */ margin: 0; /* 2 */ vertical-align: baseline; /* 3 */ *vertical-align: middle; /* 3 */
}
/**
* Address Firefox 3+ setting `line-height` on `input` using `!important` in * the UA stylesheet. */
button, input {
line-height: normal;
}
/**
* Address inconsistent `text-transform` inheritance for `button` and `select`. * All other form control elements do not inherit `text-transform` values. * Correct `button` style inheritance in Chrome, Safari 5+, and IE 6+. * Correct `select` style inheritance in Firefox 4+ and Opera. */
button, select {
text-transform: none;
}
/**
* 1. Avoid the WebKit bug in Android 4.0.* where (2) destroys native `audio` * and `video` controls. * 2. Correct inability to style clickable `input` types in iOS. * 3. Improve usability and consistency of cursor style between image-type * `input` and others. * 4. Remove inner spacing in IE 7 without affecting normal text inputs. * Known issue: inner spacing remains in IE 6. */
button, html input[type="button"], /* 1 */ input[type="reset"], input[type="submit"] {
-webkit-appearance: button; /* 2 */ cursor: pointer; /* 3 */ *overflow: visible; /* 4 */
}
/**
* Re-set default cursor for disabled elements. */
button[disabled], html input[disabled] {
cursor: default;
}
/**
* 1. Address box sizing set to content-box in IE 8/9. * 2. Remove excess padding in IE 8/9. * 3. Remove excess padding in IE 7. * Known issue: excess padding remains in IE 6. */
input[type="checkbox"], input[type="radio"] {
box-sizing: border-box; /* 1 */ padding: 0; /* 2 */ *height: 13px; /* 3 */ *width: 13px; /* 3 */
}
/**
* 1. Address `appearance` set to `searchfield` in Safari 5 and Chrome. * 2. Address `box-sizing` set to `border-box` in Safari 5 and Chrome * (include `-moz` to future-proof). */
input[type="search"] {
-webkit-appearance: textfield; /* 1 */ -moz-box-sizing: content-box; -webkit-box-sizing: content-box; /* 2 */ box-sizing: content-box;
}
/**
* Remove inner padding and search cancel button in Safari 5 and Chrome * on OS X. */
input[type="search"]::-webkit-search-cancel-button, input[type="search"]::-webkit-search-decoration {
-webkit-appearance: none;
}
/**
* Remove inner padding and border in Firefox 3+. */
button::-moz-focus-inner, input::-moz-focus-inner {
border: 0; padding: 0;
}
/**
* 1. Remove default vertical scrollbar in IE 6/7/8/9. * 2. Improve readability and alignment in all browsers. */
textarea {
overflow: auto; /* 1 */ vertical-align: top; /* 2 */
}
/* ==========================================================================
Tables ========================================================================== */
/**
* Remove most spacing between table cells. */
table {
border-collapse: collapse; border-spacing: 0;
}
/*csslint important:false*/
/* ==========================================================================
Pure Base Extras ========================================================================== */
/**
* Extra rules that Pure adds on top of Normalize.css */
/**
* Always hide an element when it has the `hidden` HTML attribute. */
[hidden] {
display: none !important;
}
/**
* Add this class to an image to make it fit within it's fluid parent wrapper while maintaining * aspect ratio. */
.pure-img {
max-width: 100%; height: auto; display: block;
}
/*csslint regex-selectors:false, known-properties:false, duplicate-properties:false*/
.pure-g {
letter-spacing: -0.31em; /* Webkit: collapse white-space between units */ *letter-spacing: normal; /* reset IE < 8 */ *word-spacing: -0.43em; /* IE < 8: collapse white-space between units */ text-rendering: optimizespeed; /* Webkit: fixes text-rendering: optimizeLegibility */
/* Sets the font stack to fonts known to work properly with the above letter and word spacings. See: https://github.com/yui/pure/issues/41/
The following font stack makes Pure Grids work on all known environments.
* FreeSans: Ships with many Linux distros, including Ubuntu
* Arimo: Ships with Chrome OS. Arimo has to be defined before Helvetica and
Arial to get picked up by the browser, even though neither is available
in Chrome OS.
* Droid Sans: Ships with all versions of Android.
* Helvetica, Arial, sans-serif: Common font stack on OS X and Windows. */ font-family: FreeSans, Arimo, "Droid Sans", Helvetica, Arial, sans-serif;
/* Use flexbox when possible to avoid `letter-spacing` side-effects.
NOTE: Firefox (as of 25) does not currently support flex-wrap, so the `-moz-` prefix version is omitted. */
display: -webkit-flex; -webkit-flex-flow: row wrap;
/* IE10 uses display: flexbox */ display: -ms-flexbox; -ms-flex-flow: row wrap;
}
/* Opera as of 12 on Windows needs word-spacing.
The ".opera-only" selector is used to prevent actual prefocus styling and is not required in markup.
- /
.opera-only :-o-prefocus, .pure-g {
word-spacing: -0.43em;
}
.pure-u {
display: inline-block; *display: inline; /* IE < 8: fake inline-block */ zoom: 1; letter-spacing: normal; word-spacing: normal; vertical-align: top; text-rendering: auto;
}
/* Resets the font family back to the OS/browser's default sans-serif font, this the same font stack that Normalize.css sets for the `body`.
- /
.pure-g [class *= "pure-u"] {
font-family: sans-serif;
}
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display: inline-block; *display: inline; zoom: 1; letter-spacing: normal; word-spacing: normal; vertical-align: top; text-rendering: auto;
}
.pure-u-1-24 {
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.pure-u-1-12, .pure-u-2-24 {
width: 8.3333%; *width: 8.3023%;
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.pure-u-1-8, .pure-u-3-24 {
width: 12.5000%; *width: 12.4690%;
}
.pure-u-1-6, .pure-u-4-24 {
width: 16.6667%; *width: 16.6357%;
}
.pure-u-1-5 {
width: 20%; *width: 19.9690%;
}
.pure-u-5-24 {
width: 20.8333%; *width: 20.8023%;
}
.pure-u-1-4, .pure-u-6-24 {
width: 25%; *width: 24.9690%;
}
.pure-u-7-24 {
width: 29.1667%; *width: 29.1357%;
}
.pure-u-1-3, .pure-u-8-24 {
width: 33%; *width: 33%;
}
.pure-u-3-8, .pure-u-9-24 {
width: 37.5000%; *width: 37.4690%;
}
.pure-u-2-5 {
width: 40%; *width: 39.9690%;
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.pure-u-5-12, .pure-u-10-24 {
width: 41.6667%; *width: 41.6357%;
}
.pure-u-11-24 {
width: 45.8333%; *width: 45.8023%;
}
.pure-u-1-2, .pure-u-12-24 {
width: 49%; *width: 49.9690%;
}
.pure-u-13-24 {
width: 54.1667%; *width: 54.1357%;
}
.pure-u-7-12, .pure-u-14-24 {
width: 58.3333%; *width: 58.3023%;
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.pure-u-3-5 {
width: 60%; *width: 59.9690%;
}
.pure-u-5-8, .pure-u-15-24 {
width: 62.5000%; *width: 62.4690%;
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.pure-u-2-3, .pure-u-16-24 {
width: 66.6667%; *width: 66.6357%;
}
.pure-u-17-24 {
width: 70.8333%; *width: 70.8023%;
}
.pure-u-3-4, .pure-u-18-24 {
width: 75%; *width: 74.9690%;
}
.pure-u-19-24 {
width: 79.1667%; *width: 79.1357%;
}
.pure-u-4-5 {
width: 80%; *width: 79.9690%;
}
.pure-u-5-6, .pure-u-20-24 {
width: 83.3333%; *width: 83.3023%;
}
.pure-u-7-8, .pure-u-21-24 {
width: 87.5000%; *width: 87.4690%;
}
.pure-u-11-12, .pure-u-22-24 {
width: 91.6667%; *width: 91.6357%;
}
.pure-u-23-24 {
width: 95.8333%; *width: 95.8023%;
}
.pure-u-1, .pure-u-1-1, .pure-u-5-5, .pure-u-24-24 {
width: 100%;
} .pure-button {
/* Structure */ display: inline-block; *display: inline; /*IE 6/7*/ zoom: 1; line-height: normal; white-space: nowrap; vertical-align: baseline; text-align: center; cursor: pointer; -webkit-user-drag: none; -webkit-user-select: none; -moz-user-select: none; -ms-user-select: none; user-select: none;
}
/* Firefox: Get rid of the inner focus border */ .pure-button::-moz-focus-inner {
padding: 0; border: 0;
}
/*csslint outline-none:false*/
.pure-button {
font-family: inherit; font-size: 100%; *font-size: 90%; /*IE 6/7 - To reduce IE's oversized button text*/ *overflow: visible; /*IE 6/7 - Because of IE's overly large left/right padding on buttons */ padding: 0.5em 1em; color: #444; /* rgba not supported (IE 8) */ color: rgba(0, 0, 0, 0.80); /* rgba supported */ *color: #444; /* IE 6 & 7 */ border: 1px solid #999; /*IE 6/7/8*/ border: none rgba(0, 0, 0, 0); /*IE9 + everything else*/ background-color: #E6E6E6; text-decoration: none; border-radius: 2px;
}
.pure-button-hover, .pure-button:hover, .pure-button:focus {
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#00000000', endColorstr='#1a000000',GradientType=0); background-image: -webkit-gradient(linear, 0 0, 0 100%, from(transparent), color-stop(40%, rgba(0,0,0, 0.05)), to(rgba(0,0,0, 0.10))); background-image: -webkit-linear-gradient(transparent, rgba(0,0,0, 0.05) 40%, rgba(0,0,0, 0.10)); background-image: -moz-linear-gradient(top, rgba(0,0,0, 0.05) 0%, rgba(0,0,0, 0.10)); background-image: -o-linear-gradient(transparent, rgba(0,0,0, 0.05) 40%, rgba(0,0,0, 0.10)); background-image: linear-gradient(transparent, rgba(0,0,0, 0.05) 40%, rgba(0,0,0, 0.10));
} .pure-button:focus {
outline: 0;
} .pure-button-active, .pure-button:active {
box-shadow: 0 0 0 1px rgba(0,0,0, 0.15) inset, 0 0 6px rgba(0,0,0, 0.20) inset;
}
.pure-button[disabled], .pure-button-disabled, .pure-button-disabled:hover, .pure-button-disabled:focus, .pure-button-disabled:active {
border: none; background-image: none; filter: progid:DXImageTransform.Microsoft.gradient(enabled = false); filter: alpha(opacity=40); -khtml-opacity: 0.40; -moz-opacity: 0.40; opacity: 0.40; cursor: not-allowed; box-shadow: none;
}
.pure-button-hidden {
display: none;
}
/* Firefox: Get rid of the inner focus border */ .pure-button::-moz-focus-inner{
padding: 0; border: 0;
}
.pure-button-primary, .pure-button-selected, a.pure-button-primary, a.pure-button-selected {
background-color: rgb(0, 120, 231); color: #fff;
}
.pure-form input[type="text"], .pure-form input[type="password"], .pure-form input[type="email"], .pure-form input[type="url"], .pure-form input[type="date"], .pure-form input[type="month"], .pure-form input[type="time"], .pure-form input[type="datetime"], .pure-form input[type="datetime-local"], .pure-form input[type="week"], .pure-form input[type="number"], .pure-form input[type="search"], .pure-form input[type="tel"], .pure-form input[type="color"], .pure-form select, .pure-form textarea {
padding: 0.5em 0.6em; display: inline-block; border: 1px solid #ccc; box-shadow: inset 0 1px 3px #ddd; border-radius: 4px; -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box;
}
/* Need to separate out the :not() selector from the rest of the CSS 2.1 selectors since IE8 won't execute CSS that contains a CSS3 selector.
- /
.pure-form input:not([type]) {
padding: 0.5em 0.6em; display: inline-block; border: 1px solid #ccc; box-shadow: inset 0 1px 3px #ddd; border-radius: 4px; -webkit-box-sizing: border-box; -moz-box-sizing: border-box; box-sizing: border-box;
}
/* Chrome (as of v.32/34 on OS X) needs additional room for color to display. */
/* May be able to remove this tweak as color inputs become more standardized across browsers. */
.pure-form input[type="color"] {
padding: 0.2em 0.5em;
}
.pure-form input[type="text"]:focus,
.pure-form input[type="password"]:focus,
.pure-form input[type="email"]:focus,
.pure-form input[type="url"]:focus,
.pure-form input[type="date"]:focus,
.pure-form input[type="month"]:focus,
.pure-form input[type="time"]:focus,
.pure-form input[type="datetime"]:focus,
.pure-form input[type="datetime-local"]:focus,
.pure-form input[type="week"]:focus,
.pure-form input[type="number"]:focus,
.pure-form input[type="search"]:focus,
.pure-form input[type="tel"]:focus,
.pure-form input[type="color"]:focus,
.pure-form select:focus,
.pure-form textarea:focus {
outline: 0; outline: thin dotted \9; /* IE6-9 */ border-color: #129FEA;
}
/* Need to separate out the :not() selector from the rest of the CSS 2.1 selectors since IE8 won't execute CSS that contains a CSS3 selector.
- /
.pure-form input:not([type]):focus {
outline: 0; outline: thin dotted \9; /* IE6-9 */ border-color: #129FEA;
}
.pure-form input[type="file"]:focus, .pure-form input[type="radio"]:focus, .pure-form input[type="checkbox"]:focus {
outline: thin dotted #333; outline: 1px auto #129FEA;
} .pure-form .pure-checkbox, .pure-form .pure-radio {
margin: 0.5em 0; display: block;
}
.pure-form input[type="text"][disabled], .pure-form input[type="password"][disabled], .pure-form input[type="email"][disabled], .pure-form input[type="url"][disabled], .pure-form input[type="date"][disabled], .pure-form input[type="month"][disabled], .pure-form input[type="time"][disabled], .pure-form input[type="datetime"][disabled], .pure-form input[type="datetime-local"][disabled], .pure-form input[type="week"][disabled], .pure-form input[type="number"][disabled], .pure-form input[type="search"][disabled], .pure-form input[type="tel"][disabled], .pure-form input[type="color"][disabled], .pure-form select[disabled], .pure-form textarea[disabled] {
cursor: not-allowed; background-color: #eaeded; color: #cad2d3;
}
/* Need to separate out the :not() selector from the rest of the CSS 2.1 selectors since IE8 won't execute CSS that contains a CSS3 selector.
- /
.pure-form input:not([type])[disabled] {
cursor: not-allowed; background-color: #eaeded; color: #cad2d3;
} .pure-form input[readonly], .pure-form select[readonly], .pure-form textarea[readonly] {
background: #eee; /* menu hover bg color */ color: #777; /* menu text color */ border-color: #ccc;
}
.pure-form input:focus:invalid, .pure-form textarea:focus:invalid, .pure-form select:focus:invalid {
color: #b94a48; border-color: #ee5f5b;
} .pure-form input:focus:invalid:focus, .pure-form textarea:focus:invalid:focus, .pure-form select:focus:invalid:focus {
border-color: #e9322d;
} .pure-form input[type="file"]:focus:invalid:focus, .pure-form input[type="radio"]:focus:invalid:focus, .pure-form input[type="checkbox"]:focus:invalid:focus {
outline-color: #e9322d;
} .pure-form select {
border: 1px solid #ccc; background-color: white;
} .pure-form select[multiple] {
height: auto;
} .pure-form label {
margin: 0.5em 0 0.2em;
} .pure-form fieldset {
margin: 0; padding: 0.35em 0 0.75em; border: 0;
} .pure-form legend {
display: block; width: 100%; padding: 0.3em 0; margin-bottom: 0.3em; color: #333; border-bottom: 1px solid #e5e5e5;
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.pure-form-stacked input[type="text"], .pure-form-stacked input[type="password"], .pure-form-stacked input[type="email"], .pure-form-stacked input[type="url"], .pure-form-stacked input[type="date"], .pure-form-stacked input[type="month"], .pure-form-stacked input[type="time"], .pure-form-stacked input[type="datetime"], .pure-form-stacked input[type="datetime-local"], .pure-form-stacked input[type="week"], .pure-form-stacked input[type="number"], .pure-form-stacked input[type="search"], .pure-form-stacked input[type="tel"], .pure-form-stacked input[type="color"], .pure-form-stacked select, .pure-form-stacked label, .pure-form-stacked textarea {
display: block; margin: 0.25em 0;
}
/* Need to separate out the :not() selector from the rest of the CSS 2.1 selectors since IE8 won't execute CSS that contains a CSS3 selector.
- /
.pure-form-stacked input:not([type]) {
display: block; margin: 0.25em 0;
} .pure-form-aligned input, .pure-form-aligned textarea, .pure-form-aligned select, /* NOTE: pure-help-inline is deprecated. Use .pure-form-message-inline instead. */ .pure-form-aligned .pure-help-inline, .pure-form-message-inline {
display: inline-block; *display: inline; *zoom: 1; vertical-align: middle;
} .pure-form-aligned textarea {
vertical-align: top;
}
/* Aligned Forms */ .pure-form-aligned .pure-control-group {
margin-bottom: 0.5em;
} .pure-form-aligned .pure-control-group label {
text-align: right; display: inline-block; vertical-align: middle; width: 10em; margin: 0 1em 0 0;
} .pure-form-aligned .pure-controls {
margin: 1.5em 0 0 10em;
}
/* Rounded Inputs */ .pure-form input.pure-input-rounded, .pure-form .pure-input-rounded {
border-radius: 2em; padding: 0.5em 1em;
}
/* Grouped Inputs */ .pure-form .pure-group fieldset {
margin-bottom: 10px;
} .pure-form .pure-group input {
display: block; padding: 10px; margin: 0; border-radius: 0; position: relative; top: -1px;
} .pure-form .pure-group input:focus {
z-index: 2;
} .pure-form .pure-group input:first-child {
top: 1px; border-radius: 4px 4px 0 0;
} .pure-form .pure-group input:last-child {
top: -2px; border-radius: 0 0 4px 4px;
} .pure-form .pure-group button {
margin: 0.35em 0;
}
.pure-form .pure-input-1 {
width: 100%;
} .pure-form .pure-input-2-3 {
width: 66%;
} .pure-form .pure-input-1-2 {
width: 50%;
} .pure-form .pure-input-1-3 {
width: 33%;
} .pure-form .pure-input-1-4 {
width: 25%;
}
/* Inline help for forms */ /* NOTE: pure-help-inline is deprecated. Use .pure-form-message-inline instead. */ .pure-form .pure-help-inline, .pure-form-message-inline {
display: inline-block; padding-left: 0.3em; color: #666; vertical-align: middle; font-size: 0.875em;
}
/* Block help for forms */ .pure-form-message {
display: block; color: #666; font-size: 0.875em;
}
/*csslint adjoining-classes:false, outline-none:false*/ /*TODO: Remove this lint rule override after a refactor of this code.*/
.pure-menu ul {
position: absolute; visibility: hidden;
}
.pure-menu.pure-menu-open {
visibility: visible; z-index: 2; width: 100%;
}
.pure-menu ul {
left: -10000px; list-style: none; margin: 0; padding: 0; top: -10000px; z-index: 1;
}
.pure-menu > ul { position: relative; }
.pure-menu-open > ul {
left: 0; top: 0; visibility: visible;
}
.pure-menu-open > ul:focus {
outline: 0;
}
.pure-menu li { position: relative; }
.pure-menu a, .pure-menu .pure-menu-heading {
display: block; color: inherit; line-height: 1.5em; padding: 5px 20px; text-decoration: none; white-space: nowrap;
}
.pure-menu.pure-menu-horizontal > .pure-menu-heading {
display: inline-block; *display: inline; zoom: 1; margin: 0; vertical-align: middle;
} .pure-menu.pure-menu-horizontal > ul {
display: inline-block; *display: inline; zoom: 1; vertical-align: middle;
}
.pure-menu li a { padding: 5px 20px; }
.pure-menu-can-have-children > .pure-menu-label:after {
content: '\25B8'; float: right; /* These specific fonts have the Unicode char we need. */ font-family: 'Lucida Grande', 'Lucida Sans Unicode', 'DejaVu Sans', sans-serif; margin-right: -20px; margin-top: -1px;
}
.pure-menu-can-have-children > .pure-menu-label {
padding-right: 30px;
}
.pure-menu-separator {
background-color: #dfdfdf; display: block; height: 1px; font-size: 0; margin: 7px 2px; overflow: hidden;
}
.pure-menu-hidden {
display: none;
}
/* FIXED MENU */ .pure-menu-fixed {
position: fixed; top: 0; left: 0; width: 100%;
}
/* HORIZONTAL MENU CODE */
/* Initial menus should be inline-block so that they are horizontal */ .pure-menu-horizontal li {
display: inline-block; *display: inline; zoom: 1; vertical-align: middle;
}
/* Submenus should still be display: block; */ .pure-menu-horizontal li li {
display: block;
}
/* Content after should be down arrow */ .pure-menu-horizontal > .pure-menu-children > .pure-menu-can-have-children > .pure-menu-label:after {
content: "\25BE";
} /*Add extra padding to elements that have the arrow so that the hover looks nice */ .pure-menu-horizontal > .pure-menu-children > .pure-menu-can-have-children > .pure-menu-label {
padding-right: 30px;
}
/* Adjusting separator for vertical menus */ .pure-menu-horizontal li.pure-menu-separator { height: 50%; width: 1px; margin: 0 7px; }
/* Submenus should be horizontal separator again */ .pure-menu-horizontal li li.pure-menu-separator { height: 1px; width: auto; margin: 7px 2px; }
/*csslint adjoining-classes:false*/
/*TODO: Remove this lint rule override after a refactor of this code.*/
/* MAIN MENU STYLING */
.pure-menu.pure-menu-open, .pure-menu.pure-menu-horizontal li .pure-menu-children {
background: #fff; /* Old browsers */ border: 1px solid #b7b7b7;
}
/* remove borders for horizontal menus */ .pure-menu.pure-menu-horizontal, .pure-menu.pure-menu-horizontal .pure-menu-heading {
border: none;
}
/* LINK STYLES */
.pure-menu a {
border: 1px solid transparent; border-left: none; border-right: none;
}
.pure-menu a, .pure-menu .pure-menu-can-have-children > li:after {
color: #777;
}
.pure-menu .pure-menu-can-have-children > li:hover:after {
color: #fff;
}
/* Focus style for a dropdown menu-item when the parent has been opened */ .pure-menu .pure-menu-open {
background: #dedede;
}
.pure-menu li a:hover,
.pure-menu li a:focus {
background: #eee;
}
/* DISABLED STATES */ .pure-menu li.pure-menu-disabled a:hover, .pure-menu li.pure-menu-disabled a:focus {
background: #fff; color: #bfbfbf;
}
.pure-menu .pure-menu-disabled > a {
background-image: none; border-color: transparent; cursor: default;
}
.pure-menu .pure-menu-disabled > a, .pure-menu .pure-menu-can-have-children.pure-menu-disabled > a:after {
color: #bfbfbf;
}
/* HEADINGS */ .pure-menu .pure-menu-heading {
color: #565d64; text-transform: uppercase; font-size: 90%; margin-top: 0.5em; border-bottom-width: 1px; border-bottom-style: solid; border-bottom-color: #dfdfdf;
}
/* ACTIVE MENU ITEM */ .pure-menu .pure-menu-selected a {
color: #000;
}
/* FIXED MENU */ .pure-menu.pure-menu-open.pure-menu-fixed {
border: none; border-bottom: 1px solid #b7b7b7;
}
/*csslint box-model:false*/ /*TODO: Remove this lint rule override after a refactor of this code.*/
.pure-paginator {
/* `pure-g` Grid styles */ letter-spacing: -0.31em; /* Webkit: collapse white-space between units */ *letter-spacing: normal; /* reset IE < 8 */ *word-spacing: -0.43em; /* IE < 8: collapse white-space between units */ text-rendering: optimizespeed; /* Webkit: fixes text-rendering: optimizeLegibility */
/* `pure-paginator` Specific styles */ list-style: none; margin: 0; padding: 0;
} .opera-only :-o-prefocus, .pure-paginator {
word-spacing: -0.43em;
}
/* `pure-u` Grid styles */ .pure-paginator li {
display: inline-block; *display: inline; /* IE < 8: fake inline-block */ zoom: 1; letter-spacing: normal; word-spacing: normal; vertical-align: top; text-rendering: auto;
}
.pure-paginator .pure-button {
border-radius: 0; padding: 0.8em 1.4em; vertical-align: top; height: 1.1em;
} .pure-paginator .pure-button:focus, .pure-paginator .pure-button:active {
outline-style: none;
} .pure-paginator .prev, .pure-paginator .next {
color: #C0C1C3; text-shadow: 0 -1px 0 rgba(0,0,0, 0.45);
} .pure-paginator .prev {
border-radius: 2px 0 0 2px;
} .pure-paginator .next {
border-radius: 0 2px 2px 0;
}
.pure-table {
/* Remove spacing between table cells (from Normalize.css) */ border-collapse: collapse; border-spacing: 0; empty-cells: show; border: 1px solid #cbcbcb;
}
.pure-table caption {
color: #000; font: italic 85%/1 arial, sans-serif; padding: 1em 0; text-align: center;
}
.pure-table td, .pure-table th {
border-left: 1px solid #cbcbcb;/* inner column border */ border-width: 0 0 0 1px; font-size: inherit; margin: 0; overflow: visible; /*to make ths where the title is really long work*/ padding: 0.5em 1em; /* cell padding */
} .pure-table td:first-child, .pure-table th:first-child {
border-left-width: 0;
}
.pure-table thead {
background: #e0e0e0; color: #000; text-align: left; vertical-align: bottom;
}
/* striping:
even - #fff (white) odd - #f2f2f2 (light gray)
- /
.pure-table td {
background-color: transparent;
} .pure-table-odd td {
background-color: #f2f2f2;
}
/* nth-child selector for modern browsers */ .pure-table-striped tr:nth-child(2n-1) td {
background-color: #f2f2f2;
}
/* BORDERED TABLES */ .pure-table-bordered td {
border-bottom: 1px solid #cbcbcb;
} .pure-table-bordered tbody > tr:last-child td, .pure-table-horizontal tbody > tr:last-child td {
border-bottom-width: 0;
}
/* HORIZONTAL BORDERED TABLES */
.pure-table-horizontal td, .pure-table-horizontal th {
border-width: 0 0 1px 0; border-bottom: 1px solid #cbcbcb;
} .pure-table-horizontal tbody > tr:last-child td {
border-bottom-width: 0;
}
Materials
- Guanidinium chloride (GdmCl)
- 47mm polycarbonate filter membranes with 10um pores
- Nuclease solution (Benzonase)
- 5% (m/v) SDS
- Distilled water
- After the induction period, add guanidinium chloride to the induction culture to reach a final concentration of 0.8M
- Incubate for 1-2 hours at 4°C prior to filtration
- Withdraw 30-50mL of the Gdm-containing culture, then vacuum filter using 47mm polycarbonate filter membranes with 10um pores
- Incubate the filtered biomass with 5mL of 8M GdmCl for 5 minutes
- Vacuum filter, then rinse 3 times with DI water
- Add 5mL of an aqueous solution (2uM MgCl2) of nuclease to the filtered biomass
- Vacuum filter to remove the liquid then rinse 3 times with 5mL of DI water
- Incubate the biomass on the filter with 5mL of 5% (m/v) SDS in water for 5 minutes
- Vacuum filter, then rinse 5 times with 5mL DI water
- Scrape the semi-purified curli from the filter paper with a flat spatula
- Lyophilize, and store at 4°C
Materials
- LB media
- Ice bucket and ice
- Temperature controlled centrifuge
- 2 polypropylene JA 10 500mL bottles
- 15% glycerol
- 0.6 mL tubes
- P1000 pipette and tips
- Stripettes and automatic pipette for larger volumes
- Shaker at 37 °C, 200rpm
- Inoculate 250mL of LB medium with PQN4.
- Incubate at 37°C, 200 rpm shaking overnight.
- Add 1mL of overnight culture to an 1L flask of LB media and incubate with shaking at 200rpm, 37C.
- Incubate until OD600 of around 0.4-0.7 is reached
- Before continuing, set up the necessary materials:
- Pre-cool centrifuge to 4°C
- Prepare ice bucket
- Chill 1L MQ water and 15% glycerol buffer on ice
- Once the cultures reach desired OD600, take them out of incubation and put them on ice for 30 minutes (the tube should feel cool).
- From here on, keep cells always cool, at or below 4°C
- After cooling, spin the tubes in a refrigerated (4°C) centrifuge for 30min at 5000rpm.
- Pour off supernatant carefully, taking care not to pour off the pellet. If the pellet is not attached to the wall after centrifugation, smear the pellet onto the wall of the tube and centrifuge again using longer centrifugation times. Re-suspend bacteria in 500mL MQ water
- Centrifuge again for 30 minutes at 5000 rpm and 4 °C temp
- Pour off supernatant again, re-suspend pellet in 250mL MQ water on ice as before.
- Centrifuge again for 30 minutes at 5000 rpm, 4 °C.
- Pour off supernatant and re-suspend pellet in 10mL ice cold 15% glycerol solution.
- Spin in 15mL tubes in large centrifuge for 15 minutes at 3400rpm, 4 °C
- Resuspend pellets in 2mL of 15% glycerol mixture
- Pipette 50ul aliquots into tubes. Label tubes properly.
- Store samples on ice for immediate use or freeze 50ul aliquots in-80°C. According to some reports, the efficiency of electrocompetent cells reduces after each freezing, so immediate use may result in highest efficiencies.
Materials
- Congo Red powder
- Brilliant Blue G250 dye
- Yeast extract powder
- Casamino acids
- Agar
- IPTG
- Antibiotic of choice
- CR stock: dissolve 1 g of Congo Red in 100 mL of water and filter sterilize. Store at 4°C.
- Brilliant Blue stock: dissolve 1 g Brilliant Blue G250 dye in 100 mL water and filter sterilize. Store at 4°C. [brilliant blue increases the colour contrast of the colonies on the agar]
- YESCA CR agar plates: 10 g/L casamino acids, 1 g/L yeast extract, and 20 g/L agar, 100 ug/mL antibiotic (for Amp; will vary depending on your antibiotic), 0.5 mM IPTG, 50 µg/mL Congo Red and 1 µg/mL Brilliant Blue. Autoclave only the dissolved agar and yeast extract, and filter sterilize the other components. Add the filtered components after autoclaved mixture has cooled.
- Pick single colonies and streak out on a YESCA CR agar plate
- To induce curli production, grow bacteria on YESCA CR agar at 26°C for 48 h
- Check the color of the bacterial colonies. Wild-type curli-producing E. coli cells stain red on YESCA CR agar, whereas curli defective mutants are usually pink or white. E. coli mutants with hyper curli production sometimes stain dark red
Materials
- Congo Red powder
- Brilliant Blue G250 dye
- Yeast extract powder
- Casamino acids
- Agar
- IPTG
- Antibiotic of choice
- CR stock: dissolve 1 g of Congo Red in 100 mL of water and filter sterilize. Store at 4°C.
- Brilliant Blue stock: dissolve 1 g Brilliant Blue G250 dye in 100 mL water and filter sterilize. Store at 4°C. [brilliant blue increases the colour contrast of the colonies on the agar]
- YESCA CR agar plates: 10 g/L casamino acids, 1 g/L yeast extract, and 20 g/L agar, 100 ug/mL antibiotic (for Amp; will vary depending on your antibiotic), 0.5 mM IPTG, 50 µg/mL Congo Red and 1 µg/mL Brilliant Blue. Autoclave only the dissolved agar and yeast extract, and filter sterilize the other components. Add the filtered components after autoclaved mixture has cooled.
- Pick single colonies and streak out on a YESCA CR agar plate
- To induce curli production, grow bacteria on YESCA CR agar at 26°C for 48 h
- Check the color of the bacterial colonies. Wild-type curli-producing E. coli cells stain red on YESCA CR agar, whereas curli defective mutants are usually pink or white. E. coli mutants with hyper curli production sometimes stain dark red
Materials
- Congo Red powder
- Brilliant Blue G250 dye
- Yeast extract powder
- Casamino acids
- Agar
- IPTG
- Antibiotic of choice
- CR stock: dissolve 1 g of Congo Red in 100 mL of water and filter sterilize. Store at 4°C.
- Brilliant Blue stock: dissolve 1 g Brilliant Blue G250 dye in 100 mL water and filter sterilize. Store at 4°C. [brilliant blue increases the colour contrast of the colonies on the agar]
- YESCA CR agar plates: 10 g/L casamino acids, 1 g/L yeast extract, and 20 g/L agar, 100 ug/mL antibiotic (for Amp; will vary depending on your antibiotic), 0.5 mM IPTG, 50 µg/mL Congo Red and 1 µg/mL Brilliant Blue. Autoclave only the dissolved agar and yeast extract, and filter sterilize the other components. Add the filtered components after autoclaved mixture has cooled.
- Pick single colonies and streak out on a YESCA CR agar plate
- To induce curli production, grow bacteria on YESCA CR agar at 26°C for 48 h
- Check the color of the bacterial colonies. Wild-type curli-producing E. coli cells stain red on YESCA CR agar, whereas curli defective mutants are usually pink or white. E. coli mutants with hyper curli production sometimes stain dark red
