Difference between revisions of "Team:WashU StLouis/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p style="font-size:4vw; text-align:center"> Safety </p>
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    <p style="font-size:2vw; text-align:center"> Lab Space </p>
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    <p>Officially, our team operates under the Energy, Environmental, and Chemical Engineering (EECE) Department at Washington University in St. Louis. The majority of our wet lab work is done in the Tang and Zhang Labs, both of which are <strong>Biosafety Level 1 (BSL 1)</strong>. Both labs are equipped with open bench space and biosafety cabinets, and they share 4°C, 30°C, and 37°C rooms for cell cultures and plates. Within the Zhang and Tang labs there are also safety showers, eyewash stations and fire extinguishers throughout. The Zhang lab designates a specific area and enforces safety measures for work involving the use of Ethidium Bromide (such as in gel electrophoresis). In addition to wearing proper lab attire and designated gloves within Ethidium Bromide areas, while working with or around the EtBr we only used designated lab equipment and instrumentation. Any waste connected to EtBr was disposed of in designated EtBr waste containers.</p>
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    <img src="https://static.igem.org/mediawiki/2017/8/8b/T--WashU_StLouis--LabPicOne.jpg" style="width:40%;margin:2vw">
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    <img src="https://static.igem.org/mediawiki/2017/9/91/T--WashU_StLouis--LabPicTwo.jpg" style="width:40%; margin:2vw">
  
 
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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    <p style="font-size:2vw; text-align:center"> Safety Training </p>
  
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    <p style="text-align:center">Prior to starting lab work, we took a mandatory online training course and attend a lab-specific safety training session. The online training required the completion of a lengthy slideshow and a safety quiz in order to receive certification from WashU's Environmental Health and Safety (EHS) department. Our lab-specific training was given by graduate students in the Zhang and Tang labs. During the lab-specific training, we learned the specific location of safety equipment and other materials as well as the various safety protocols instituted by both labs, including safety equipment training and hazardous waste disposal protocols.</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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    <p style="font-size:2vw; text-align:center"> Personal Protective Equipment </p>
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    <p style="text-align:center">Our PPE falls in line with the requirements of BSL 1 laboratories. This means our attire entering the lab includes long pants, appropriate closed-toe shoes, and shirts with sleeves. Once we enter the lab, we also put on fitting nitrile gloves, lab coats, and goggles/glasses when necessary.</p>
  
 
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<h5>Safe Shipment</h5>
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    <p style="font-size:2vw; text-align:center"> Chassis/Organisms Used </p>
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    <p>DH5α Escherichia Coli cells</p>
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    <p>MHD42 Escherichia Coli cells</p>
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    <p>Synechocystis PCC 6803</p>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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    <p style="font-size:2vw; text-align:center"> Oversight </p>
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    <p style="text-align:center">The development of our project is overseen by Dr. Janie Brennan and Eugene Kim, both of whom we formally meet biweekly. During these meetings, Dr. Brennan makes sure that the direction of our research is environmentally safe. In the wet lab, the Zhang Lab and Tang Lab graduate students, including Eugene, oversee our work and make sure that we are conducting our experiments safely.</p>
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    <p style="font-size:2vw; text-align:center"> Technique and Waste </p>
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    <p style="text-align:center">While working in lab we followed all the safety protocols put forth by the Zhang and Tang labs and the WUSTL EHS department. We properly disposed of all hazardous material in the appropriately labeled containers. Cultures containing bacterial cells were bleached and disposed of accordingly. Sterile technique was utilized when handling cultures of bacteria,
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or any equipment and media that would eventually come in contact with bacteria.</p>
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Latest revision as of 07:55, 24 August 2017

HOME
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Judging Form

Safety

Lab Space

Officially, our team operates under the Energy, Environmental, and Chemical Engineering (EECE) Department at Washington University in St. Louis. The majority of our wet lab work is done in the Tang and Zhang Labs, both of which are Biosafety Level 1 (BSL 1). Both labs are equipped with open bench space and biosafety cabinets, and they share 4°C, 30°C, and 37°C rooms for cell cultures and plates. Within the Zhang and Tang labs there are also safety showers, eyewash stations and fire extinguishers throughout. The Zhang lab designates a specific area and enforces safety measures for work involving the use of Ethidium Bromide (such as in gel electrophoresis). In addition to wearing proper lab attire and designated gloves within Ethidium Bromide areas, while working with or around the EtBr we only used designated lab equipment and instrumentation. Any waste connected to EtBr was disposed of in designated EtBr waste containers.

Safety Training

Prior to starting lab work, we took a mandatory online training course and attend a lab-specific safety training session. The online training required the completion of a lengthy slideshow and a safety quiz in order to receive certification from WashU's Environmental Health and Safety (EHS) department. Our lab-specific training was given by graduate students in the Zhang and Tang labs. During the lab-specific training, we learned the specific location of safety equipment and other materials as well as the various safety protocols instituted by both labs, including safety equipment training and hazardous waste disposal protocols.

Personal Protective Equipment

Our PPE falls in line with the requirements of BSL 1 laboratories. This means our attire entering the lab includes long pants, appropriate closed-toe shoes, and shirts with sleeves. Once we enter the lab, we also put on fitting nitrile gloves, lab coats, and goggles/glasses when necessary.

Chassis/Organisms Used

DH5α Escherichia Coli cells

MHD42 Escherichia Coli cells

Synechocystis PCC 6803

Oversight

The development of our project is overseen by Dr. Janie Brennan and Eugene Kim, both of whom we formally meet biweekly. During these meetings, Dr. Brennan makes sure that the direction of our research is environmentally safe. In the wet lab, the Zhang Lab and Tang Lab graduate students, including Eugene, oversee our work and make sure that we are conducting our experiments safely.

Technique and Waste

While working in lab we followed all the safety protocols put forth by the Zhang and Tang labs and the WUSTL EHS department. We properly disposed of all hazardous material in the appropriately labeled containers. Cultures containing bacterial cells were bleached and disposed of accordingly. Sterile technique was utilized when handling cultures of bacteria, or any equipment and media that would eventually come in contact with bacteria.