Difference between revisions of "Team:Queens Canada/Safety"

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<img class="bioleft" align="left" src="https://static.igem.org/mediawiki/2017/c/cf/T--Queens_Canada--Fluorescence.png" height="auto" width="35%" style="object-fit:contain;padding:10px 50px 10px 10px;">
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<Div style="position: absolute; top:1400px; left:110px; width:400px; height:25px">Agar plate streaked with negative control device (left) and positive control<br> device (right) viewed under UV light. Looks like they work!</Div>
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<h1><font color="black" face="Arial"><center><span style="font-weight:normal; font-size: 23pt">Background</span></center></font></h1><hr/>
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<p class="big"><font size="5" color="black"><br>In 2017, the Queen's University iGEM team is participating in the Fourth International Interlaboratory Measurement Study for their first time. The Interlab Study aims to analyze and improve the replicability of fluorescence measurements. This year, the reliability of some RBS devices (BCDs) will be tested all around the world, using the expression of green fluorescent protein (GFP) to quantify translation.<br><br> Participating iGEM teams measure fluorescence exhibited by the GFP across six test devices, each with different RBS sequences. A positive and negative control are also used to calculate expression levels using fluorescence/OD600. Reproducibility is one of the most challenging and critical aspects of scientific research. We hope that the data from this study can help establish a baseline for GFP measurement reproducibility, given GFP's widespread use as a tool in molecular biology. This collaborative effort is a small but meaningful step towards this goal.</font></p>
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<p class="big"><font size="5" face="Corbel"> The ability to reproduce results in biological systems is difficult due to the stochastic nature of living cells and inconsistent laboratory practices [1]. Comparing quantitative results between experiments is often difficult with many variables impacting the results. These may include:</font></p>
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<ul><font size="4" face="Lucida Sans Unicode"><li>The various instruments used and their different calibrations</li>
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<li>Variation in laboratory practices/protocols</li>
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<li>Systematic variability e.g. differences in strains used, physical laboratory conditions</li>
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<li>Variation in interpreting and communicating results</li>
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Revision as of 18:24, 28 August 2017

Background


The ability to reproduce results in biological systems is difficult due to the stochastic nature of living cells and inconsistent laboratory practices [1]. Comparing quantitative results between experiments is often difficult with many variables impacting the results. These may include:

  • The various instruments used and their different calibrations
  • Variation in laboratory practices/protocols
  • Systematic variability e.g. differences in strains used, physical laboratory conditions
  • Variation in interpreting and communicating results
    •

Safety

Please visit the main Safety page to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.

On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device
Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?