Difference between revisions of "Team:UNOTT/Design2"
| Line 89: | Line 89: | ||
<h3 style="text-align: center;"><span style="color: #D74214;"> Plasmid Design </span></h3> | <h3 style="text-align: center;"><span style="color: #D74214;"> Plasmid Design </span></h3> | ||
<p style="text-align: center;"><span style="color: #ffffff;">___________________________</span></p> | <p style="text-align: center;"><span style="color: #ffffff;">___________________________</span></p> | ||
| − | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/ | + | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/5/56/UNOTT2017-plasmiddescript.png" alt="" width="800" height="400" /> </p> |
Revision as of 00:14, 14 September 2017
.
KEY PLASMID DESIGN
Random Brick formation (Components of plasmid)
___________________________
These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator "T". This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal.
Brick stitching
___________________________
These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown.
Plasmid Design
___________________________
