Difference between revisions of "Team:Bielefeld-CeBiTec/Project/translational system/library and selection"
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+ | <b>Yuval Nov</b>, (2011). When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis. Applied and Environmental Microbiology 099-2240/12/12.00. | ||
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Revision as of 08:45, 11 October 2017
Library and Selection
Tyrosine and Pyrosyl-Lysine tRNA/aminoacyl-synthetase
Tyrosine tRNA/aminoacyl-synthetase
Figure 1: Tyrosine-binding site in apo M. jannaschii TyrRS
The electrostatic distribution around the tyrosine-binding site is shown. Positive potential is blue (10 mV), neutral potential (0 mV) is white, and negative potential (−10 mV) is red (Zhang et al.2005).
Pyrosyl-Lysine tRNA/aminoacyl-synthetase
Generating the Library
Selection
Modification of the aaRS
Figure 2: Adaption of the aminoacyl-synthetase.
Positive and negative selection for the specificity of the orthogonal synthetase for the ncAA in E. coli.