Difference between revisions of "Team:Bielefeld-CeBiTec/Results/translational system/library and selection"

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<h2> Generating the library </h2>
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The integration of a new amino acid in the translational cycle implies the creation of a large number of tRNA/aminoacyl tRNA synthetase (tRNA/aaRS) variants. This can be achieved by generating a library. This library serves as a basis for selective processes or screenings, where an optimal tRNA/aaRS can be obtained.
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The library is based on the pSB1C3 plasmid, with the tyrosyl-tRNA synthetase (TyrRS) under control of a glnS promoter inserted between the iGEM BioBrick prefix and suffix.
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For the cloning of the vector, we used the primers 17jj and 17jk for the amplification of the pSB1C3 backbone and the primers 17hq and 17ht for the amplification of the TyrRS.
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<p class="figure subtitle"><b>Figure 1: Library plasmid pSB1C3 containing the tyrosyl tRNA synthetase.</b></p>
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Revision as of 07:52, 11 October 2017

Library and Selection

Generating the library

The integration of a new amino acid in the translational cycle implies the creation of a large number of tRNA/aminoacyl tRNA synthetase (tRNA/aaRS) variants. This can be achieved by generating a library. This library serves as a basis for selective processes or screenings, where an optimal tRNA/aaRS can be obtained. The library is based on the pSB1C3 plasmid, with the tyrosyl-tRNA synthetase (TyrRS) under control of a glnS promoter inserted between the iGEM BioBrick prefix and suffix. For the cloning of the vector, we used the primers 17jj and 17jk for the amplification of the pSB1C3 backbone and the primers 17hq and 17ht for the amplification of the TyrRS.

Figure 1: Library plasmid pSB1C3 containing the tyrosyl tRNA synthetase.