Difference between revisions of "Team:Hong Kong UCCKE/Results"
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<p style="text-align:left !important;">From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After then we sent the parts to BGI for sequencing. The result is shown as above. | <p style="text-align:left !important;">From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After then we sent the parts to BGI for sequencing. The result is shown as above. | ||
| − | By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other. | + | By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other. </p> |
Base on the fact we can conclude that we have successfully cloned | Base on the fact we can conclude that we have successfully cloned | ||
| − | <p style="text-align:left !important;">Here are the results: | + | <p style="text-align:left !important;">Here are the results:<br> |
| − | + | From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour) | |
</p> | </p> | ||
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Revision as of 12:03, 17 October 2017
From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After then we sent the parts to BGI for sequencing. The result is shown as above. By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other.
Base on the fact we can conclude that we have successfully clonedHere are the results:
From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour)
Project 300
Project 400
https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png
https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png
Project 500
https://static.igem.org/mediawiki/2017/6/63/T--Hong_Kong_UCCKE--_project_500_screenshot_1.png
https://static.igem.org/mediawiki/2017/b/be/T--Hong_Kong_UCCKE--_project_500_screenshot_2.png
Project 502
https://static.igem.org/mediawiki/2017/4/48/T--Hong_Kong_UCCKE--_project_502_screenshot_1.png
https://static.igem.org/mediawiki/2017/4/40/T--Hong_Kong_UCCKE--_project_502_screenshot_2.png
