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<div class="container"> | <div class="container"> | ||
<div class="row"><div class="col-md-8"> | <div class="row"><div class="col-md-8"> | ||
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− | By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other. </p> | + | <p style="text-align:left !important;">From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After then we sent the parts to BGI for sequencing. The result is shown as above. |
− | Base on the fact we can conclude that we have successfully cloned | + | By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other.</p> |
− | <p style="text-align:left !important;">Here are the results:< | + | |
− | From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour) | + | <p style="text-align:left !important;">Base on the fact we can conclude that we have successfully cloned</p> |
− | </p> | + | |
+ | <p style="text-align:left !important;">Here are the results:</p> | ||
+ | |||
+ | <p style="text-align:left !important;">From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour)</p> | ||
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<h3 class="sectiontitle">Project 300</h3> | <h3 class="sectiontitle">Project 300</h3> | ||
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+ | <div class="divider"></div> | ||
+ | |||
<div class="row"> | <div class="row"> | ||
− | <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2017/1/1b/T--Hong_Kong_UCCKE--_project_300_screenshot_1.png" style="width:100%;"/ | + | |
− | + | <div class="col-sm-6"> | |
+ | <img src="https://static.igem.org/mediawiki/2017/1/1b/T--Hong_Kong_UCCKE--_project_300_screenshot_1.png" style="width:100%;"/> | ||
</div> | </div> | ||
+ | |||
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/16/T--Hong_Kong_UCCKE--_project_300_screenshot_2.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
<h3 class="sectiontitle" style="clear:both;">Project 400</h3> | <h3 class="sectiontitle" style="clear:both;">Project 400</h3> | ||
<div class="divider"></div> | <div class="divider"></div> | ||
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− | < | + | <div class="row"> |
+ | |||
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/2d/T--Hong_Kong_UCCKE--_project_400_screenshot_1.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/e1/T--Hong_Kong_UCCKE--_project_400_screenshot_2.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
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+ | |||
<h3 class="sectiontitle" style="clear:both;">Project 500</h3> | <h3 class="sectiontitle" style="clear:both;">Project 500</h3> | ||
<div class="divider"></div> | <div class="divider"></div> | ||
− | + | <div class="row"> | |
− | < | + | |
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/63/T--Hong_Kong_UCCKE--_project_500_screenshot_1.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/be/T--Hong_Kong_UCCKE--_project_500_screenshot_2.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
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+ | |||
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<h3 class="sectiontitle" style="clear:both;">Project 502</h3> | <h3 class="sectiontitle" style="clear:both;">Project 502</h3> | ||
<div class="divider"></div> | <div class="divider"></div> | ||
− | + | <div class="row"> | |
− | < | + | |
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/48/T--Hong_Kong_UCCKE--_project_502_screenshot_1.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/40/T--Hong_Kong_UCCKE--_project_502_screenshot_2.png" style="width:100%;"/> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
<div class="col-md-4"> | <div class="col-md-4"> | ||
</div> | </div> |
Revision as of 12:06, 17 October 2017
From CU we cloned cells that contained our part. Then we did miniprep hence purified the plasmid. After then we sent the parts to BGI for sequencing. The result is shown as above. By subtracting 1000 bp of the template sequence aligned with our own sequence. It is found that the both matches with each other.
Base on the fact we can conclude that we have successfully cloned
Here are the results:
From the pictures below, the continuous grey line formed by rectangle boxes represents the theoretical sequence from the IDT,. Below it is the sequence of the gene we cloned. While there are flaws such as mutations along the gene we cloned (highlighted in red colour)