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| | <div style="height: 200px; width: 100%;"> | | <div style="height: 200px; width: 100%;"> |
| | </br></br> | | </br></br> |
| − | <p>No lab work done</p> | + | <p>We did stuff</p> |
| | </div> | | </div> |
| | </div> | | </div> |
| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <ul>
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| − | <li>First day in lab!</li>
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| − | <li>Made 1L of LB Media</li>
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| − | </ul>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
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| − | <li>Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803</li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Designed PCR primers for <i>pgk</i>, <i>pck</i>, <i>fldA</i>, <i>petF</i>, ATP Synthase 1, and ATP Synthase 2</li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <ul>
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| − | <li>Primers Arrived</li>
| |
| − | <li>Ran PCR on ATP Synthase 2 , <i><i>pgk</i></i>, <i><i>fldA</i></i>, <i><i>petF</i></i>, and pdCas9 plasmid</li>
| |
| − | <li>Ran all PCR products on gel and recovered ATP Synthase 2, <i><i>fldA</i></i>, and <i><i>petF</i></i></li>
| |
| − | <li>Ran gradient PCR on pdCas9 plasmid</li>
| |
| − | </ul>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − |
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| − | <div style="height: 200px; width: 100%;">
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| − |
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li> Made and ran gel for pdCas9</li>
| |
| − | <li>Ran PCR on ATP Synthase 1, <i><i>pgk</i></i>, and <i><i>pck</i></i></li>
| |
| − | <li>Performed gel extraction on yesterday’s ATP Synthase 2</li>
| |
| − | <li>Ran <i><i>pck</i></i>, <i><i>pgk</i></i>, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes</li>
| |
| − | <li>Performed gel extraction on pdCas9, <i>fldA</i>, <i>petF</i></li>
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| − | <li>Ran PCR on psc101 and psc102 plasmid</li>
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| − | <li>Ran psc101 and psc102 PCR products on gel - were recovered</li>
| |
| − | <li>Ran gradient PCR on ATP Synthase 1</li>
| |
| − | <li>Made agar plates (forgot the antibiotic)</li>
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| − | <li>Ran golden gate one pot assembly for <i>fldA</i> <i>petF</i> genes and the pdCas9 plasmid</li>
| |
| − | </ul>
| |
| − | </div>
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| − |
| |
| − | </div>
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| − |
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| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran ATP Synthase 1 PCR products on gel - no bands seen</li>
| |
| − | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
| |
| − | <li>Ran PCR on ATP Synthase 1 (3rd times the charm)</li>
| |
| − | <li>Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED</li>
| |
| − | <li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction</li>
| |
| − | <li>Made new agar plates (with the chloramphenicol)</li>
| |
| − | <li>Transformed the <i>petF</i>, <i>fldA</i> plasmids into competent cells</li>
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| − | <li>Plated colonies on agar plates and set in incubator</li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Checked for colonies (both genes were successful)</li>
| |
| − | <li>Picked colonies and incubated overnight</li>
| |
| − | <li>Ran yesterday’s PCR products on gel - bands were light but all were recovered</li>
| |
| − | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
| |
| − | <li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction</li>
| |
| − | <li>Ran PCR products on gel - all were recovered</li>
| |
| − | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprepped plasmids for <i>fldA</i>, <i>petF</i></li>
| |
| − | <li>Ran sequence PCR on both plasmids</li>
| |
| − | <li>Ran PCR product on gel to confirm</li>
| |
| − | <li>Clean and Concentrated PCR product</li>
| |
| − | <li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction (for the fourth time)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Prepared DNA for sequencing</li>
| |
| − | <li>Delivered DNA to WashU Med School</li>
| |
| − | <li>Added DPnI to psc101 and psc102 gel extract products, incubated for an hour, did Clean and Concentrate, measured conc</li>
| |
| − | <li>Ran Golden Gate assembly on <i>pck</i>, <i>pgk</i>, and ATP Synthase</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Electroporation with golden gate products</li>
| |
| − | <li>Redid golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase (did not run properly first time)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Electroporation with better golden gate products</li>
| |
| − | <li>Picked colonies for worse golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprep worse ATP, <i>pck</i>, <i>pgk</i> golden gate</li>
| |
| − | <li>Seq PCR worse ATP, <i>pck</i>, <i>pgk</i> golden gate</li>
| |
| − | <li>Picked colonies for better golden gate for <i>pck</i>, <i>pgk</i>, ATP synthase</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
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| − |
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| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Made frozen stock of better golden gate <i>pck</i>, <i>pgk</i>, ATP synthase</li>
| |
| − | <li>Miniprepped better golden gate <i>pck</i>, <i>pgk</i>, ATP synthase (low concentration)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
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| − |
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| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran sequencing PCR for <i>pgk</i>, <i>pck</i> (4 colonies each)</li>
| |
| − | <li>Gel Extraction for <i>pgk</i>, <i>pck</i> PCR product (no bands)</li>
| |
| − | </ul> </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran sequencing PCR for <i>pck</i> and <i>pgk</i> on a gradient</li>
| |
| − | <li>Gel Extraction for <i>pck</i> PCR product was successful, not <i>pgk</i></li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
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| − | <ul>
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| − | <li>Ran sequencing PCR for <i>pgk</i> on a different gradient</li>
| |
| − | <li>Gel Extraction for <i>pgk</i> PCR product was not successful</li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
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| − | <p>No lab work done</p>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Picked four new colonies of <i>pgk</i></li>
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| − | <li>Started 4 new cultures from frozen stock</li>
| |
| − | </ul>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
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| − | </br></br>
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| − | <ul>
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| − | <li>Made frozen stock of the 4 new colonies</li>
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| − | <li>Miniprepped all 8 <i>pgk</i> cultures and measured concentrations</li>
| |
| − | <li>Ran gradient PCR on all 8 <i>pgk</i> plasmids</li>
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| − | <li>Prepped and sent ATP synthase and <i>pck</i> in for sequencing</li>
| |
| − | </ul>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
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| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
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| − | <p>No lab work done</p>
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| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
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| − | <li>Mixed new <i>pgk</i> sequencing primers</li>
| |
| − | </ul>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran gradient PCR on all 8 <i>pgk</i> plasmids</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
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| − | <p>No lab work done</p>
| |
| − | </div>
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| − | </div>
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| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
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| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> 4th of July! </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Sequencing results for ATP and <i>pck</i> were not good</li>
| |
| − | <li>Reran seq PCR on ATP Synthase (bad results)</li>
| |
| − | <li>Picked new ATP colonies</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprepped overnight cultures of ATP 5-8 and forgot to make frozen stock</li>
| |
| − | <li>Ran PCR on ATP plasmids 5-8</li>
| |
| − | <li>PCR for <i>pgk</i> with new primers with gradient</li>
| |
| − | <li>Ran <i>pgk</i> PCR product on gels</li>
| |
| − | <li>Ran golden gate on <i>pgk</i> PCR gel extract</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Electroporation with golden gate <i>pgk</i> product</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Picked 4 <i>pgk</i> products and inoculated them overnight</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Made frozen stock of new 4 <i>pgk</i> cultures</li>
| |
| − | <li>Miniprepped cultures</li>
| |
| − | <li>Seq PCR on new cultures</li>
| |
| − | <li>All 4 cultures showed correct bands</li>
| |
| − | <li>Clean and Concentrate <i>pgk</i> PCR product</li>
| |
| − | <li>Prepped <i>pgk</i> for sequencing</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Delivered <i>pgk</i> for sequencing</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>New ATP synthase sequencing primers and <i>pck</i> regular primers came in</li>
| |
| − | <li>Seq PCR of ATP synthase plasmids with new sequencing primers. No bands</li>
| |
| − | <li>Picked 4 new ATP synthase colonies (again)</li>
| |
| − | <li>Inoculated MG1655 from frozens stock to make more genomic DNA</li>
| |
| − | </ul> </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Extracted genomic DNA from MG1655</li>
| |
| − | <li>Miniprepped new ATP cultures</li>
| |
| − | <li>Ran sequencing PCR on new ATP and got no bands at all. Will use newly transformed cells tomorrow</li>
| |
| − | <li>Ran PCR with <i>pck</i> primers, got light bands</li>
| |
| − | <li>Reran PCR with <i>pck</i> products</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran <i>pck</i> on gel</li>
| |
| − | <li>Gel extracted <i>pck</i> and combined gel extracts to improve concentration</li>
| |
| − | <li>Golden gate ligation on <i>pck</i></li>
| |
| − | <li>Ran ATP sequencing PCR again with different backbone primers</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran gel from yesterday’s ATP PCR. No bands</li>
| |
| − | <li>Ran PCR for <i>pfo</i></li>
| |
| − | <li>Incubated <i>pck</i> and BioBrick cells</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprepped <i>pck</i> and biobrock genes (HSP)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran ATP seq PCR gradient again. No bands. Done with ATP</li>
| |
| − | <li>BioBrick digestion, ligation, transformation with HSP and GFP</li>
| |
| − | <li>Ran PCR seq for <i>pck</i> and gel extract</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Delivered <i>pck</i> sequence to med school</li>
| |
| − | <li>Ligated backbone for BioBrick</li>
| |
| − | <li>Extracted more genomic DNA from MG1655</li>
| |
| − | <li>Gradient PCR on <i>pfo</i></li>
| |
| − | <li>Received 𝛥<i>aceE</i> cells from Yale</li>
| |
| − | <li>Rehydrated and incubated 𝛥<i>aceE</i> cells</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran PCR and gel extract for <i>fldA</i> backbone and <i>petF</i> backbone</li>
| |
| − | <li>DPn1 the two backbones</li>
| |
| − | <li>golden gate ligation on <i>pfo</i> gene and two backbones</li>
| |
| − | <li>Streaked colony from 𝛥<i>aceE</i> plate onto LB/kan plate</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Making media for competent cell protocol</li>
| |
| − | <li>Made new LB with MgSO4 for chemical transformation</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Digested BioBrick miniprep to check length</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Chemically competent cell protocol with 𝛥<i>aceE</i></li>
| |
| − | <li>Miniprepped <i>pfo</i>-<i>petF</i> and <i>fldA</i>-<i>petF</i></li>
| |
| − | <li>Seq PCR miniprepped <i>pfo</i>-<i>petF</i> and <i>fldA</i>=<i>petF</i></li>
| |
| − | <li>Remade LB with MgSO4 with correct concentration</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Restarted comp cell protocol (3rd times the charm)</li>
| |
| − | <li>Clean and Concentrate junction 2 for <i>pfo</i>-<i>fldA</i></li>
| |
| − | <li>Conducted HSP test in LB</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Redid HSP test in minimal media M9</li>
| |
| − | <li>Recultured BioBrick and DH10B control</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Reset up BioBrick experiment but grew diluted cells with LB instead of M9, so we have to do it again</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Redid HSP test in minimal media M9</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Chemical transformation of <i>pfo</i>-<i>petF</i>, <i>pfo</i>-<i>fldA</i>, <i>petF</i>, <i>fldA</i> into knockout strain, plated 80 and 240 ul, also control</li>
| |
| − | <li>Part 1 of interlab study (LUDOX)</li>
| |
| − | <li>Transforming 5 interlab plasmids</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Picked and incubated electron donor colonies plated yesterday</li>
| |
| − | <li>Picked and incubated interlab colonies plated yesterday (2 per construct)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p><ul>
| |
| − | <li>Made frozen stock of incubated colonies and interlab parts</li>
| |
| − | <li>Redid BioBrick experiment at 37C</li>
| |
| − | </ul></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Started induction but had to stop bc no frozen stock of <i>fldA</i>-<i>pfo</i> or <i>petF</i>-<i>fldA</i> in DH10B</li>
| |
| − | <li>Transformed <i>fldA</i>-<i>pfo</i> and <i>petF</i>-<i>pfo</i> into DH10B</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Made frozen stock of 𝛥<i>aceE</i></li>
| |
| − | <li>Made 60% ethanol</li>
| |
| − | <li>Incubated 8 constructs for biotin assay w/ methanol extraction (4 constructs and blank in DH10B and 𝛥<i>aceE</i>)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Methanol extraction assay (grew cells and froze pellets)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Assayed <i>petF</i>-<i>aceE</i> cells. Absorbance was too low to be meaningful</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran preliminary sonication test</li>
| |
| − | <li>Incubated large volume of DH10B</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran large sonication test on DH10B to decide protocol</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Incubated large volume of DH10B</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>More sonication tests</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Made ATP standards from assay instructions</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Made more LB</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Inoculated <i>pgk</i>, <i>pck</i>, DH10B for assay tomorrow</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>ATP luminescence assay</li>
| |
| − | <li>Inoculated 4 constructs and blank in 𝛥<i>aceE</i> cells for tomorrow</li>
| |
| − | </ul> </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Nothing grew, couldn’t do induction</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Inoculated DH10B constructs and control from frozen stock</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>DH10B electron donor induction, froze pellets, measured OD600</li>
| |
| − | <li>Re-transformed constructs into 𝛥<i>aceE</i> cells</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Sonicated and assayed pellets for biotin (1 minute, 40 amp, 1mL PBS)</li>
| |
| − | <li>Reinoculated 𝛥<i>aceE</i> constructs in all antibiotic combinations to determine problem, in LB and plates</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Visited Monsanto</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Measured absorbance data from biotin in DH10B cells</li>
| |
| − | <li>Did ATP Assay and got luminescence data</li>
| |
| − | <li>Incubated all four 𝛥<i>aceE</i> electron donor constructs from frozen stock plates and original plates and incubated 𝛥<i>aceE</i> and DH10B</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Nothing grew from yesterday's inoculations</li>
| |
| − | <li>Interlab study</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Remade competent cells</li>
| |
| − | <li>Finished interlab study (FITC) and sent to iGEM HQ</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Picked colonies from 4 new 𝛥<i>aceE</i> plates and incubated</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>𝛥<i>aceE</i> cell induction</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Incubated <i>pck</i>, <i>pgk</i>, DH10B from frozen stock</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran ATP assay</li>
| |
| − | <li>BioBrick digestion and ligation</li>
| |
| − | <li>Sonicated 𝛥<i>aceE</i> pellets</li>
| |
| − | <li>Ran 𝛥<i>aceE</i> cell absorbance scan</li>
| |
| − | <li>Ran biotin assay on 𝛥<i>aceE</i> cells</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Transformed ligated <i>petF</i> and <i>pck</i> BioBrick plasmids into DH10B (wrong antibiotic)</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Transformed ligated <i>petF</i> and <i>pck</i> BioBrick plasmids into DH10B (kan = right)</li>
| |
| − | <li>Wrapped <i>pck</i> and <i>pgk</i> plasmids to be sent to Cardiff University iGEM</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Small colonies grew on all plates</li>
| |
| − | <li>Picked colonies and inoculated</li>
| |
| − | <li>Sent plasmids to Cardiff University iGEM</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprep on <i>pck</i> and <i>petF</i> BioBrick</li>
| |
| − | <li>Reusupended TetR-pTet gBlocks</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Digestion/Ligation/Transformation of pSB1C3 and tetr-ptet BioBrick</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>TetR-pTet colonies grew, picked colonies</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Miniprepped TetR-pTet BioBrick</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>digested BioBricks to check bands sizes</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>No lab work done</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran PCR on BioBrick plasmids</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran PCR products on gel</li>
| |
| − | <li>Only <i>petF</i> showed up</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>digestion of <i>pck</i>, TetR-pTet, and pSB1C3 with EcoRI</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Digestion of <i>pck</i>, TetR-pTet, and pSB1C3 with PstI</li>
| |
| − | <li>Ligated <i>pck</i> and TetR-pTet to pSB1C3</li>
| |
| − | <li>Transformed into DH10B</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Only TetR-pTet grew</li>
| |
| − | <li>Ran colony PCR with 8 <i>pck</i> colonies and 8 TetR-pTet colonies</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Ran colony PCR products on a gel but got no bands</li>
| |
| − | <li>Miniprepped pTet1-8</li>
| |
| − | <li>Ran PCR on pTet 1-8 plasmids to check if insert was present</li>
| |
| − | <li>Ran PCR product on gel, got the band!</li>
| |
| − | <li>Prepped <i>petF</i>, <i>pck</i>, and TetR-pTet to be sent to iGEM HQ</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <ul>
| |
| − | <li>Last day in lab!</li>
| |
| − | <li>Sent parts to iGEM!</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>WIKIFREEZE</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>Done in lab</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Prepping for Jamboree </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Prepping for Jamboree </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>Prepping for Jamboree</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Prepping for Jamboree </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Prepping for Jamboree </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Prepping for Jamboree </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>Fly to Boston for Jamboree</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> GIANT JAMBOREE</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>GIANT JAMBOREE</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p>PRESENTATION</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="mySlides fade">
| |
| − | <div style="height: 200px; width: 100%;">
| |
| − | </br></br>
| |
| − | <p> Fly back to St. Louis</p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | </div>
| |
| | | | |
| | | | |
| − | </div>
| |
| | | | |
| | | | |
