Difference between revisions of "Team:UCSC/Acetaminophen"
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| − | + | <p>We aim to genetically modify PCC 7942 to produce acetaminophen, a common mild anesthetic and antipyretic recognized by the WHO as an essential medicine <sup>[1]</sup>. However, in many countries with lower regulations and faulty policies regarding drug manufacturing, acetaminophen can be synthesized with lethal toxins that result in hundreds of deaths worldwide <sup>[23]</sup>. Acetaminophen is oftenused in conjunction with opioid pain medications postoperatively to enhance pain relief, thus reducing reliance upon opioid pharmaceuticals <sup>[24]</sup>.</p> | |
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<figcaption>Current synthetic biology approach to manufacturing acetaminophen in <span style="font-style: italic";>E. coli</span> <sup>[25, 26]</sup>. Genes <span style="font-style: italic";>4ABH</span> and <span style="font-style: italic";>nhoA</span> were inserted to synthesize the pathway in PCC 7942. The gene from <span style="font-style: italic";>A. bisporus, 4ABH</span>, produces 4-aminophenol while the <span style="font-style: italic";>E. coli</span> gene <span style="font-style: italic";>nhoA</span> converts that 4-aminophenol to acetaminophen <sup>[25]</sup>.</figcaption> | <figcaption>Current synthetic biology approach to manufacturing acetaminophen in <span style="font-style: italic";>E. coli</span> <sup>[25, 26]</sup>. Genes <span style="font-style: italic";>4ABH</span> and <span style="font-style: italic";>nhoA</span> were inserted to synthesize the pathway in PCC 7942. The gene from <span style="font-style: italic";>A. bisporus, 4ABH</span>, produces 4-aminophenol while the <span style="font-style: italic";>E. coli</span> gene <span style="font-style: italic";>nhoA</span> converts that 4-aminophenol to acetaminophen <sup>[25]</sup>.</figcaption> | ||
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| − | + | <p>We are using a previously engineered pathway in <span style="font-style: italic";>E. coli</span> as a model of acetaminophen biosynthesis to enhance PCC 7942 <sup>[26, 25]</sup>. The pathway converts chorismate, an abundant aminoacid precursor of tryptophan, phenylalanine, and tyrosine, into acetaminophen with the addition ofthe <span style="font-style: italic";>4ABH</span> gene from <span style="font-style: italic";>A. bisporus</span>, an edible mushroom, and <span style="font-style: italic";>nhoA</span> from <span style="font-style: italic";>E. coli</span>.</p> | |
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Revision as of 21:26, 20 October 2017
Acetaminophen Metabolics
"Antipyretic drugs, by being analgesics, reduce not only the fever but also the pain."
~Clinical Manual of Fever in Children
We aim to genetically modify PCC 7942 to produce acetaminophen, a common mild anesthetic and antipyretic recognized by the WHO as an essential medicine [1]. However, in many countries with lower regulations and faulty policies regarding drug manufacturing, acetaminophen can be synthesized with lethal toxins that result in hundreds of deaths worldwide [23]. Acetaminophen is oftenused in conjunction with opioid pain medications postoperatively to enhance pain relief, thus reducing reliance upon opioid pharmaceuticals [24].
We are using a previously engineered pathway in E. coli as a model of acetaminophen biosynthesis to enhance PCC 7942 [26, 25]. The pathway converts chorismate, an abundant aminoacid precursor of tryptophan, phenylalanine, and tyrosine, into acetaminophen with the addition ofthe 4ABH gene from A. bisporus, an edible mushroom, and nhoA from E. coli.
