Difference between revisions of "Team:WashU StLouis/Notebook"

Line 522: Line 522:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em>Micah, Maddie, Alex</em></p>
 
     <p><em>Micah, Maddie, Alex</em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
         <li> Prepared LB Media </li>
 
         <li> Prepared LB Media </li>
Line 529: Line 530:
 
         <li> Prepared overnight culture to make competent cells (MHD42) </li>
 
         <li> Prepared overnight culture to make competent cells (MHD42) </li>
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 536: Line 538:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em>Micah, Maddie</em></p>
 
     <p><em>Micah, Maddie</em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
         <li> Prepared MHD42 competent cells </li>
 
         <li> Prepared MHD42 competent cells </li>
Line 545: Line 548:
 
         <li> Prepared TSS Buffer </li>
 
         <li> Prepared TSS Buffer </li>
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 566: Line 570:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em>Micah, Maddie, Alex, Mark, Collin</em></p>
 
     <p><em>Micah, Maddie, Alex, Mark, Collin</em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
         <li> Prepared Chloramphenicol (CM) plates </li>
 
         <li> Prepared Chloramphenicol (CM) plates </li>
Line 571: Line 576:
 
         <li> Made more LB Agar </li>
 
         <li> Made more LB Agar </li>
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 578: Line 584:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em>Micah, Maddie, Alex, Mark, Collin</em></p>
 
     <p><em>Micah, Maddie, Alex, Mark, Collin</em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
         <li> Prepared DH5α competent cells</li>
 
         <li> Prepared DH5α competent cells</li>
Line 583: Line 590:
 
         <li> Tested DH5α cells for competency</li>
 
         <li> Tested DH5α cells for competency</li>
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 590: Line 598:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 600: Line 610:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 610: Line 622:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 620: Line 634:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 630: Line 646:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 640: Line 658:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 650: Line 670:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 660: Line 682:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 670: Line 694:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 680: Line 706:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 690: Line 718:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 700: Line 730:
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><em></em></p>
 
     <p><em></em></p>
 +
    <p><strong> Lab Work </strong></p>
 
     <ol>
 
     <ol>
 
        
 
        
 
     </ol>
 
     </ol>
 +
    <p><strong> Outside Work / Discussion </strong><p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>

Revision as of 03:39, 7 June 2017

HOME
Team
Project
Background
Description
Design
Model
Results
Demonstrate
Improve
Attributions
Citations
Wet Lab
Notebook
Experiments/Protocols
InterLab
Safety
Parts
Parts
Basic Parts
Composite Parts
Human Practices
Silver HP
Public Engagement
Awards
Hardware
Model
Judging Form

Notebook

June 2017
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

Micah, Maddie, Alex

Lab Work

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Outside Work / Discussion

Micah, Maddie

Lab Work

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

Outside Work / Discussion

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Outside Work / Discussion

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion