Lichenyuan (Talk | contribs) |
Lichenyuan (Talk | contribs) |
||
Line 119: | Line 119: | ||
<h2 id="section-1" style="padding-top: 100px; margin-top: -50px;">DNA gel extraction</h2> | <h2 id="section-1" style="padding-top: 100px; margin-top: -50px;">DNA gel extraction</h2> | ||
<h4> | <h4> | ||
− | 1. Excise the agarose gel slice, transfer the gel slice into a 1.5ml microfuge tube. | + | 1. Excise the agarose gel slice, transfer the gel slice into a 1.5ml microfuge tube.<br /> 2. Add a 3X sample |
volume of Buffer DE-A. the weight of gel is equivalent to a 100ul volume.<br /> 3. Resuspend the gel in Buffer | volume of Buffer DE-A. the weight of gel is equivalent to a 100ul volume.<br /> 3. Resuspend the gel in Buffer | ||
DE-A by vortexing. Heat at 75℃ until the gel is completely dissolved(no more than 10 min)<br /> 4. Add 0.5X Buffer | DE-A by vortexing. Heat at 75℃ until the gel is completely dissolved(no more than 10 min)<br /> 4. Add 0.5X Buffer |
Revision as of 15:04, 21 October 2017