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/> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance.<br /> 4. 2YT medium<br /> The recipe | /> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance.<br /> 4. 2YT medium<br /> The recipe | ||
for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L<br /> 5. 0.5mM | for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L<br /> 5. 0.5mM | ||
− | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃<br /> 6. 50mM PBS buffer,PH8.0<br /> A:0.05mol/L | + | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃<br /> 6. 50mM PBS buffer,PH8.0<br /> A:0.05mol/L Na2HPO4<br |
− | /> B:0.05mol/L | + | /> B:0.05mol/L KH2P04<br /> 137mMNaCl,2.7mMKCl,10mMNa2HPO4,2mMKH2PO4 for 1L.<br /> |
</h4> | </h4> |
Revision as of 02:43, 22 October 2017