Difference between revisions of "Team:Freiburg/Basic Part"

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<p>Our project involved the characterization of different promoters as well as their signaling inputs. Their unique properties make them not only applicable to many medical purposes but they can also be used for any other field necessitating input dependent expression. Hence we want to share the sequences encoding the main parts of our CARTEL<sup>TM</sup> AND gate. Future iGEM teams will have the opportunity to take advantage of our research if they are planning to work with pH, hypoxia as well as VEGF. Most of the sequences were obtained by literature research and have been synthesized by <a href="https://eu.idtdna.com/site" target="_blank">Integrated DNA technologies</a>, engaging their special offer for iGEM teams. Also sequences have been purchased on <a href="https://www.addgene.org/" target="blank">Addgene</a> and have been produced via amplification of cDNA. Recognition sites for restriction enzymes used for standard cloning have been removed without causing amino acid changes. Cloning our inserts into pSB1C3, was done using <a href="https://2017.igem.org/Team:Freiburg/Methods">extension PCR and Gibson Assembly</a>.</p>
  
  
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Revision as of 16:05, 28 October 2017

Logo
Overview Motivation CAR T Cells Tumor Microenvironment AND Gate Outlook Achievements References
Members Attributions Partners Contact
Main Project Modeling Applied Design Proof of Concept BioBricks Basic Part BioBrick Improvement Interlab Study
Safety Cloning HIF1A Knockdown Cell Culture Lab Book
Human Practice Integrated Human Practice Collaborations

Basic Part

Our project involved the characterization of different promoters as well as their signaling inputs. Their unique properties make them not only applicable to many medical purposes but they can also be used for any other field necessitating input dependent expression. Hence we want to share the sequences encoding the main parts of our CARTELTM AND gate. Future iGEM teams will have the opportunity to take advantage of our research if they are planning to work with pH, hypoxia as well as VEGF. Most of the sequences were obtained by literature research and have been synthesized by Integrated DNA technologies, engaging their special offer for iGEM teams. Also sequences have been purchased on Addgene and have been produced via amplification of cDNA. Recognition sites for restriction enzymes used for standard cloning have been removed without causing amino acid changes. Cloning our inserts into pSB1C3, was done using extension PCR and Gibson Assembly.