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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> | <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/Silver">Silver</a> </li> | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li> |
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold_Integrated</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li> |
<li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> | <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li> |
<li class="dropdown active"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | <li class="dropdown active"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | ||
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</h4> | </h4> | ||
<h2 id="section-6" style="padding-top: 100px; margin-top: -50px;">Knock out the genes of Saccharomyces cerevisiae with Crispr-Cas9</h2> | <h2 id="section-6" style="padding-top: 100px; margin-top: -50px;">Knock out the genes of Saccharomyces cerevisiae with Crispr-Cas9</h2> | ||
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<img src="https://static.igem.org/mediawiki/2017/c/c6/Sxt_%284%29.png" style="max-width:60%;"></a> | <img src="https://static.igem.org/mediawiki/2017/c/c6/Sxt_%284%29.png" style="max-width:60%;"></a> | ||
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<h2 id="section-9" style="padding-top: 100px; margin-top: -50px;">Point mutation</h2> | <h2 id="section-9" style="padding-top: 100px; margin-top: -50px;">Point mutation</h2> | ||
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<img src="https://static.igem.org/mediawiki/2017/0/0f/Sxt_%289%29.png" style="max-width:60%;"></a> | <img src="https://static.igem.org/mediawiki/2017/0/0f/Sxt_%289%29.png" style="max-width:60%;"></a> | ||
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<h2 id="section-10" style="padding-top: 100px; margin-top: -50px;">Reagents</h2> | <h2 id="section-10" style="padding-top: 100px; margin-top: -50px;">Reagents</h2> | ||
<h4>1. LB medium(lysogeny broth)<br /> The recipe for 1l LB media is as follows:<br /> Tryptone 10g/L <br /> Yeast | <h4>1. LB medium(lysogeny broth)<br /> The recipe for 1l LB media is as follows:<br /> Tryptone 10g/L <br /> Yeast | ||
− | extract 5g/L <br /> NaCl 10g/L | + | extract 5g/L <br /> NaCl 10g/L<br /> 2. 0.1mM Kanamycin<br /> MW of Kanamycin:582.58<br /> Store at -20℃<br /> 3. LB plate<br /> The recipe for 1l LB plate is as follows:<br /> Tryptone 10g/L <br /> Yeast extract 5g/L <br |
− | /> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance. | + | /> NaCl 10g/L<br /> 15g Agar<br /> Add appropriate amount of resistance.<br /> 4. 2YT medium<br /> The recipe |
− | for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L | + | for 1l LB plate is as follows:<br /> Tryptone 16g/L <br /> Yeast extract 10g/L <br /> NaCl 5g/L<br /> 5. 0.5mM |
− | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃ | + | IPTG <br /> MW of IPTG:238.30<br /> Store at -20℃<br /> 6. 50mM PBS buffer,PH8.0<br /> A:0.05mol/L Na2HPO4<br |
/> B:0.05mol/L KH2P04<br /> 137mMNaCl,2.7mMKCl,10mMNa2HPO4,2mMKH2PO4 for 1L.<br /> | /> B:0.05mol/L KH2P04<br /> 137mMNaCl,2.7mMKCl,10mMNa2HPO4,2mMKH2PO4 for 1L.<br /> | ||
Revision as of 12:59, 24 October 2017