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Revision as of 07:06, 23 October 2017
INTERLAB
![](https://static.igem.org/mediawiki/2017/8/80/Unott_interlab_plate.jpeg)
Introduction
is organised by iGEM and is aimed at tackling a prominent issue regarding experimental
fluorescent reporter data. At present, experiments using fluorescent reporters are almost
incomparable; separate groups often interpret fluorescence measurements in largely different
ways, making it hard to properly compare results. To combat this, the interlab study is
performed each year, iteratively refining an optimal protocol for fluorescence measurement.
Theoretically, this would be followed by anyone and yield comparably robust units of
fluorescence, facilitating more meaningful comparisons of data between researchers.
The study required us to measure a number of different fluorescent test devices sent to us by iGEM HQ, we followed their protocol and then shared the results we obtain. Teams around the world do the same; collectively, we provide a large set of data illustrating how similar the results were, potentially highlighting any aspects in which the protocol could be improved and, hopefully, showing that this is possible. This is quite an important issue, we felt we should participate and help in any way we could!
Methods
Results
LUDOX-S40 and Fluoroscein Reference Measurements
Standard Results
Interpretive Data