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<td>98 (denaturation)<br> | <td>98 (denaturation)<br> | ||
45-72 (annealing) <a href="#Note1">see Note 1</a><br> | 45-72 (annealing) <a href="#Note1">see Note 1</a><br> | ||
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Revision as of 04:23, 24 October 2017
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
??? | ??? | ??? |
---|---|---|
14.3 | H2O | 114.4 |
2.5 | 98 (denaturation) 45-72 (annealing) see Note 1 72 (extension) |
5-10 seconds 10-30 seconds 15-30 seconds per kb |
Final extension | 72 | 2-5 minutes |
Hold | 4 | Indefinitely |