Difference between revisions of "Team:Gaston Day School/Notebook"

Line 61: Line 61:
 
         <tr>
 
         <tr>
 
             <td>2.5</td>
 
             <td>2.5</td>
             <td>98 (denaturation)<br>
+
             <td>Buffer</td>
                45-72 (annealing) <a href="#Note1">see Note 1</a><br>
+
             <td>20</td>
                72 (extension)</td>
+
             <td>5-10 seconds <br>
+
                10-30 seconds<br>
+
                15-30 seconds per kb</td>
+
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
             <td>Final extension</td>
+
             <td>1</td>
             <td>72</td>
+
             <td>MgCl2</td>
             <td>2-5 minutes</td>
+
             <td>8</td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
             <td>Hold</td>
+
             <td>1</td>
             <td>4</td>
+
             <td>dNTP</td>
             <td>Indefinitely</td>
+
             <td>8</td>
 
         </tr>
 
         </tr>
 
 
     </tbody>
 
     </tbody>
 
</table>
 
</table>

Revision as of 04:24, 24 October 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

??? ??? ???
14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8