Labels

For MCF tubes:

1. P: Plasmid Prep
2. D: Digest
3. T: Transformation
4. L: Ligation

Transformation

1. x = the number of transformations you are doing
2. Place the SOC medium (from the fridge) in the incubator
3. Also place X plates in the incubator (With the appropriate antibiotic resistance, Chl for complete, Amp for parts)
4. Make an ice-water bath
5. Obtain X MCF tubes and a float
6. Add 50 ul of competent cells
7. Add 2ul of the DNA
8. Place it in the ice-water bath for 2 mins
9. While waiting, turn on the sink's hot water, obtain a beaker, add hot water, and using a thermometer, adjust water to 42o C (using the hot plate if needed)
10. Place tubes in the incubator for 30 seconds
11. Another 2 mins on ice
12. Add 1 ml of warm SOC medium
13. Place each tube in the incubator (37o C) for 1 hour
14. Plating