Difference between revisions of "Team:Nanjing-China/Parts"

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       <p>SQR is a NAD(P)/FAD-dependent  oxidoreductase, which oxidizes S2- to zero-valent S0.  SqrR, encoded by <em>sqrR</em>, is a  constitutively expressed suppressor protein of <em>sqr</em> promotor. RFP is the indicator. When there is no H2S  in the environment, SqrR combines with the <em>sqr</em> promotor and keeps the expression of both the enzyme and RFP at a low level. While  in the case where H2S exists, it will be oxidized by the small  amount of expressed enzyme, and the product will interact with SqrR, forming a  tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of  SqrR. The change in the structure of SqrR makes it fall off from the <em>sqr</em> promotor and thus the expression of  SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.</p>
 
       <p>SQR is a NAD(P)/FAD-dependent  oxidoreductase, which oxidizes S2- to zero-valent S0.  SqrR, encoded by <em>sqrR</em>, is a  constitutively expressed suppressor protein of <em>sqr</em> promotor. RFP is the indicator. When there is no H2S  in the environment, SqrR combines with the <em>sqr</em> promotor and keeps the expression of both the enzyme and RFP at a low level. While  in the case where H2S exists, it will be oxidized by the small  amount of expressed enzyme, and the product will interact with SqrR, forming a  tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of  SqrR. The change in the structure of SqrR makes it fall off from the <em>sqr</em> promotor and thus the expression of  SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.</p>
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Revision as of 07:40, 26 October 2017

Team:Nanjing-China - 2017.igem.org

H2: BBa_K2257000

SQR is a NAD(P)/FAD-dependent oxidoreductase, which oxidizes S2- to zero-valent S0. SqrR, encoded by sqrR, is a constitutively expressed suppressor protein of sqr promotor. RFP is the indicator. When there is no H2S in the environment, SqrR combines with the sqr promotor and keeps the expression of both the enzyme and RFP at a low level. While in the case where H2S exists, it will be oxidized by the small amount of expressed enzyme, and the product will interact with SqrR, forming a tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of SqrR. The change in the structure of SqrR makes it fall off from the sqr promotor and thus the expression of SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.

H2S: BBa_K2257006

SQR is a NAD(P)/FAD-dependent oxidoreductase, which oxidizes S2- to zero-valent S0. SqrR, encoded by sqrR, is a constitutively expressed suppressor protein of sqr promotor. RFP is the indicator. When there is no H2S in the environment, SqrR combines with the sqr promotor and keeps the expression of both the enzyme and RFP at a low level. While in the case where H2S exists, it will be oxidized by the small amount of expressed enzyme, and the product will interact with SqrR, forming a tri- or tetrasulfide cross-links linking C41 and C107 on the same subunit of SqrR. The change in the structure of SqrR makes it fall off from the sqr promotor and thus the expression of SQR and RFP turns on. The part can be used to detect hydrogen sulfide at a μmol level.

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Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

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