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<a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | ||
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+ | <h4>The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardware | ||
+ | to model the industrial production and enhance our method. However, the gap does exist between the | ||
+ | reality and our expectation. | ||
+ | </h4> | ||
− | < | + | <h3 style="text-align:center">List of what we use</h3> |
− | <h4> | + | <h4>By listing the main items we have purchased and actually used, we hope to provide some experience and |
− | the | + | inspiration for the future team wishing to have HW production. |
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− | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/19/Hardware_tab.png" width="60%" height="60%" > | |
+ | </div> | ||
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+ | <h3 style="text-align:center">Hardware Design</h3> | ||
− | <h4> | + | <h4>Our system is mainly composed of two parts: reaction part and concentrated crystallization part. The |
− | + | overall idea is that the reaction part provides the bacteria with appropriate reaction temperature, | |
− | + | adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the | |
− | + | reaction solution will be transferred to the concentrated crystallization part, where the concentration | |
− | + | of the productivity is increased by the reverse osmosis membrane; the temperature of the reaction | |
− | + | solution is lowered by the device, and the acrylic acid is finally crystallized. | |
− | + | </h4> | |
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+ | <h4>1)Reaction Part</h4> | ||
− | < | + | <h4>The reaction part mainly uses miniature DC self-priming pump, foam machine, acrylic material container, |
− | </ | + | aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor. |
+ | </h4> | ||
− | <h4> | + | <h4> |
− | + | (图)Fig1 Hontainer made of Acrylics Fig2 Hollow cylinder made of aluminum alloy Fig3Miniature DC self - priming pump Fig4 | |
+ | 3D design drawing | ||
+ | </h4> | ||
− | < | + | <h4>Instructions:When the system starts, the pump will continue to pump the upper reaction solution, which |
− | + | will blend a large amount of air bubbles after passing the foam machine, and then will be pumped | |
− | + | back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and | |
− | + | the bubbles are continuously rising, the purpose of stirring the reaction solution and providing | |
− | are | + | sufficient air can be achieved. Simultaneously, with the help of the temperature sensor, the system |
− | + | will be able to control heating wire inside the aluminum alloy cylinder, so that the reaction solution | |
+ | can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials | ||
+ | is conducive to insulation. (We had considered heating the air mixed with the reaction solution at | ||
+ | the foaming machine so that the reaction liquid pumped back to the reaction vessel would be blended | ||
+ | with a large amount of hot air, which could replace the additional heating device, but due to the | ||
+ | safety and energy efficiency, this thought was given up.) | ||
</h4> | </h4> | ||
+ | <h4>2)Concentrated Crystallization Part</h4> | ||
− | + | <h4>After the reaction has been performed for one cycle, the reaction part stops, and the reaction solution | |
− | + | is thus introduced into the concentrated crystallization part and the work begins. | |
− | + | <br> In this part we mainly use the acrylic material container, aluminum alloy cold source (including | |
− | <h4> | + | collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor |
− | + | and semiconductor refrigeration film, etc. | |
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</h4> | </h4> | ||
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− | <h4> | + | <h4> |
− | + | (图)Fig5 Hontainer made of Acrylics Fig6 Cold source produced by aluminum alloy Fig 7 Reverse osmosis RO membrane Fig9 3D | |
− | + | design drawing | |
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</h4> | </h4> | ||
+ | <h4>Instructions:Concentrated crystallization part is relatively independent. As long as the system detects | ||
+ | that the internal water level is higher than the reverse osmosis membrane, the part will start to | ||
+ | work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will | ||
+ | be continuously extracted, which continues to improve the concentration of acrylic acid. And the | ||
+ | semiconductor refrigeration film will help keep the solution temperature below the melting point | ||
+ | 286K of acrylic acid. After many rounds of cycles, acrylic acid crystals can be collected on the | ||
+ | bottom cooling source (but mixed with engineered bacteria). | ||
+ | <br> 3)System Controlling | ||
+ | <br> In order to keep as much as possible in line with the actual industrial production, our device involves | ||
+ | a lot of electronic components, pumps, etc., so we designed a set of controlling procedures. | ||
+ | <br> Because of our lack of experience and staff, we chose to master the Arduino controlling board due | ||
+ | to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display, | ||
+ | Relay module, NTC thermistor temperature sensor, Adjustable DC power supply, Semiconductor refrigeration | ||
+ | chip, Arduino controlling panel, Water level sensor, DuPont line, LCD1602 LCD adapter board, Motor | ||
+ | drive extension and so on. | ||
+ | <br> Here is our main program of the controlling system: | ||
+ | </h4> | ||
+ | (压缩文件) | ||
+ | <h3 style="text-align:center">Video</h3> | ||
+ | <h3 style="text-align:center">Impression summary</h3> | ||
− | + | <h4>We have no relevant laboratory and company support, staff is also very scarce (the main staff is only | |
+ | one person). The road of our first HW work was quite stumbling. So in this part, we hope to be able | ||
+ | to provide a lesson that feels most profound: | ||
+ | <br> Before the actual production and debugging, full consideration and deduction must be carried out. | ||
+ | The delivery of the things bought often cost two or three days (in China, we mainly do online shopping, | ||
+ | so the courier time needs to be considered), and the purchase of goods may be inconsistent with what | ||
+ | we wanted. Plus, the negligence of some important details may lead to the purchase of wrong things, | ||
+ | which will waste a lot of valuable time. | ||
+ | <br> If you want to know more or cooperate with us, welcome to contact us | ||
+ | </h4> | ||
</div> | </div> |
Revision as of 10:46, 27 October 2017