Difference between revisions of "Team:MSU-Michigan/Parts"

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<h5>Adding parts to the registry</h5>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
  
  
  
 
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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<p>The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on http://regprecise.lbl.gov/RegPrecise/.  Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence.  This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted.  All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.</p>
 
<p>The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on http://regprecise.lbl.gov/RegPrecise/.  Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence.  This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted.  All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.</p>

Revision as of 14:49, 27 October 2017

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Parts

The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on http://regprecise.lbl.gov/RegPrecise/. Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence. This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted. All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.

Part Table

Biobricks

"Confirmation of promoters induced by (from left to right) Blue light, Paraquat, Nitrate (NO3), and Copper"

<groupparts>iGEM17 MSU-Michigan</groupparts>