Difference between revisions of "Team:Arizona State/Notebook"

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<center><img src="https://static.igem.org/mediawiki/2017/6/65/BjaR_PCR.png" alt="Design Flowchart" style="max-width: 800px; width: 100%"></center>
 
<center><img src="https://static.igem.org/mediawiki/2017/6/65/BjaR_PCR.png" alt="Design Flowchart" style="max-width: 800px; width: 100%"></center>
 
   
 
   
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<h2>Friday 9/26/17 - PCR Cleanup of BjaR</h2>
  
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<h3> PCR Cleanup </h3>
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<p> The volume of this PCR is 128 uL. The following is the protocl for PCR cleanup in lab. </p>
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<ul>
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<li> Binding</li>
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          <li> Add 5x Volume of PB </li>
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          <li> Add 1/10 X Volume of PCR results of NaAc pH 5.3 </li>
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          <li> Spin through PCR Cleanup tube at maximum speed for 1 minute and dump the flow through</li>
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<li> Wash</li>
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          <li> Add 750 uL of PE wash solution </li>
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          <li> Spin through column at maximum speed for 1 minute </li>
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          <li> Dump the flow through liquid into waster container </li>
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<li> Spin Again and dump flow through liquid</li>
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<li> Transfer PCR Column to a new epindorph tube </li>
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          <li> Add 35 uL of distilled water and let it sit for 1 minutes </li>
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          <li> Spin to elute at maximum speed for 1 minute </li>
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<li> Test the Concentration in (ng/uL) </li>
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<p> The concentration of BjaR was 123.12 ng/uL </p>
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</ul>
  
  

Revision as of 18:53, 28 October 2017