Difference between revisions of "Team:Macquarie Australia/Results"
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| − | <h1> | + | <center> <h1>Aim</h1> </center> |
| + | <br> | ||
| + | <ul> | ||
| + | <li>We designed and ordered the gBlocks for 4 genes, which encoded for proteins involved in hydrogen production (<i>fer</i>, <i>hyd1</i>, <i>hydEF</i>, <i>hydG</i>) from <i>Chlamydomonas reinhardtii</i>. </li> | ||
| + | <li>Improved gBlock <i>hydG</i> which demonstrated a loss of functionality (2016) due to a point mutation.</li> | ||
| + | <li>These gBlocks were inserted into one Biobrick (known as the Hydrogen Producing Gene Cluster) and transformed into <i>Escherichia coli</i> with a <i>lac</i> promoter and chloramphenicol resistance. </li> | ||
| + | <li>Once induced, we aimed to test the rate of hydrogen gas production in these cells.<li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <br> | ||
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<p align="justify">Here you can describe the results of your project and your future plans. </p> | <p align="justify">Here you can describe the results of your project and your future plans. </p> | ||
Revision as of 10:35, 29 October 2017
Aim
- We designed and ordered the gBlocks for 4 genes, which encoded for proteins involved in hydrogen production (fer, hyd1, hydEF, hydG) from Chlamydomonas reinhardtii.
- Improved gBlock hydG which demonstrated a loss of functionality (2016) due to a point mutation.
- These gBlocks were inserted into one Biobrick (known as the Hydrogen Producing Gene Cluster) and transformed into Escherichia coli with a lac promoter and chloramphenicol resistance.
- Once induced, we aimed to test the rate of hydrogen gas production in these cells.
Here you can describe the results of your project and your future plans.
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