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Revision as of 14:18, 28 October 2017
![](https://static.igem.org/mediawiki/2017/0/03/T--UNOTT--Results.jpeg)
RESULTS:
OBJECTIVE: CREATE pgRNA
OBJECTIVE: CREATE pREPORTER
OBJECTIVE: PROMOTER LIBRARY
OBJECTIVE: RANDOM LIGATIONS
OBJECTIVE: FREEZE DRYING
OBJECTIVE: CRISPRi & gRNA EFFICIENCY
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.
![](https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg)