Difference between revisions of "Team:Hong Kong UCCKE/Contribution"
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| − | <h3 class="sectiontitle"> | + | <h3 class="sectiontitle">BBa_K2197600</h3> |
<div class="divider"></div> | <div class="divider"></div> | ||
<p style="text-align:left !important;">This composite part consists of two sessions which is the promoter and BBa_K1456006 (designed by Mustafa Semih Elitok iGEM14_ATOMS-Turkiye). The promoter HucR which is a bacterial transcriptional repressor will dissociates from the binding site hucO8 in the presence of uric acid and allow the expression of BBa_K1456006, Aprotinin.</p> | <p style="text-align:left !important;">This composite part consists of two sessions which is the promoter and BBa_K1456006 (designed by Mustafa Semih Elitok iGEM14_ATOMS-Turkiye). The promoter HucR which is a bacterial transcriptional repressor will dissociates from the binding site hucO8 in the presence of uric acid and allow the expression of BBa_K1456006, Aprotinin.</p> | ||
| − | <p style="text-align:left !important;">Aprotinin is a potent protease inhibitor which prevents the formation of xanthine oxidase (XO) from xanthine dehydrogenase (XDH). </p><br> | + | <a href="https://static.igem.org/mediawiki/2017/2/27/T--Hong_Kong_UCCKE--600sequence.jpg" data-caption="Image caption"> |
| − | <a href="https://static.igem.org/mediawiki/parts/ | + | <figure><img src="https://static.igem.org/mediawiki/2017/2/27/T--Hong_Kong_UCCKE--600sequence.jpg" alt=""></figure> |
| + | </a><br> | ||
| + | <h3 class="sectiontitle" style="clear:both;">How does it work?</h3> | ||
| + | <div class="divider"></div> | ||
| + | <p style="text-align:left !important;">The promoter is designed to be sensitive to the concentration of uric acid. This promoter control the expression of Aprotinin that is downstream the promoter. The promoter session consists of a constitutive promoter J23100, a RBS B0034, a strong repressor KRAB-HucR, a double terminator B0015. HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS). hucO is an operative site for mUTS to bind. When mUTS is binded to hucO, the expression of downstream gene is restricted according concentration of substrates. The presence of uric acid limits the binding of mUTS to hucO. The limitation varies as the concentration of uric acid. Downstream of the promoter session is a constitutive promoter J23106, a RBS B0034, a gene K1456006 and a double terminator B0015. As a result mUTS binds to hucO and GFP is not expressed when uric acid is absent or at very low concentration. Alternatively, the complex detaches from hucO and GFP is expressed according to the concentration of uric acid. The promoter control the expression of aprotinin.</p><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/4/4e/T--Hong_Kong_UCCKE--600noua.jpg" data-caption="Image caption"> | ||
| + | <figure><img src="https://static.igem.org/mediawiki/2017/4/4e/T--Hong_Kong_UCCKE--600noua.jpg" alt=""></figure> | ||
| + | </a><br> | ||
| + | <p style="text-align:left !important;">Expression with uric acid (+UA)</p><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/8/8b/T--Hong_Kong_UCCKE--realbindsitea.jpg" data-caption="Image caption"> | ||
| + | <figure><img src="https://static.igem.org/mediawiki/2017/8/8b/T--Hong_Kong_UCCKE--realbindsitea.jpg" alt=""></figure> | ||
| + | </a><br> | ||
| + | <p style="text-align:left !important;">Expression without uric acid (+UA)</p><br> | ||
| + | <p style="text-align:left !important;">As Xanthine Oxidase (XO) is an enzyme that catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. Aprotinin is a potent protease inhibitor which prevents the formation of xanthine oxidase (XO) from xanthine dehydrogenase (XDH). Therefore, theoretically, when there are higher uric acid level in blood, more aprotinin will be expressed and less xanthine or uric acid will be produced by oxidation.</p><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/7/72/T--Hong_Kong_UCCKE--newpathforpurine.jpg" data-caption="Image caption"> | ||
| + | <figure><img src="https://static.igem.org/mediawiki/2017/7/72/T--Hong_Kong_UCCKE--newpathforpurine.jpg" alt=""></figure> | ||
| + | </a><br> | ||
| + | <p style="text-align:left !important;">The pathway from purine to uric acid</p><br> | ||
| + | <a href="https://static.igem.org/mediawiki/parts/d/d9/T--Hong_Kong_UCCKE--aprotinin.jpg" data-caption="Image caption"> | ||
<figure><img src="https://static.igem.org/mediawiki/parts/d/d9/T--Hong_Kong_UCCKE--aprotinin.jpg" alt=""></figure> | <figure><img src="https://static.igem.org/mediawiki/parts/d/d9/T--Hong_Kong_UCCKE--aprotinin.jpg" alt=""></figure> | ||
| − | </a><br><p> | + | </a><br> |
| + | <p style="text-align:left !important;">Aprotinin stopping oxidation from XDH to XO</p><br> | ||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
Revision as of 15:52, 28 October 2017
BBa_K2197600
This composite part consists of two sessions which is the promoter and BBa_K1456006 (designed by Mustafa Semih Elitok iGEM14_ATOMS-Turkiye). The promoter HucR which is a bacterial transcriptional repressor will dissociates from the binding site hucO8 in the presence of uric acid and allow the expression of BBa_K1456006, Aprotinin.
How does it work?
The promoter is designed to be sensitive to the concentration of uric acid. This promoter control the expression of Aprotinin that is downstream the promoter. The promoter session consists of a constitutive promoter J23100, a RBS B0034, a strong repressor KRAB-HucR, a double terminator B0015. HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS). hucO is an operative site for mUTS to bind. When mUTS is binded to hucO, the expression of downstream gene is restricted according concentration of substrates. The presence of uric acid limits the binding of mUTS to hucO. The limitation varies as the concentration of uric acid. Downstream of the promoter session is a constitutive promoter J23106, a RBS B0034, a gene K1456006 and a double terminator B0015. As a result mUTS binds to hucO and GFP is not expressed when uric acid is absent or at very low concentration. Alternatively, the complex detaches from hucO and GFP is expressed according to the concentration of uric acid. The promoter control the expression of aprotinin.
Expression with uric acid (+UA)
Expression without uric acid (+UA)
As Xanthine Oxidase (XO) is an enzyme that catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. Aprotinin is a potent protease inhibitor which prevents the formation of xanthine oxidase (XO) from xanthine dehydrogenase (XDH). Therefore, theoretically, when there are higher uric acid level in blood, more aprotinin will be expressed and less xanthine or uric acid will be produced by oxidation.
The pathway from purine to uric acid
Aprotinin stopping oxidation from XDH to XO
