Difference between revisions of "Team:SHSBNU China/Notebook"

 
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         <div class="post-75 post type-post status-publish format-standard hentry category-azir-quantum-science concavebox">
 
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                 <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Project</p>
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                 <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Notebook</p>
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                 <p></p>
+
                 <p>SHSBNU_China iGEM2017 would like to share with you the document of the work done every week for our project. Although we have only 6 team members in the team, we go all out to do the experiments.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week1</p>
 +
                <p><strong>10/07/2017-16/07/2017</strong></p>
 +
                <p>Do Interlab experiment at Peking iGEM team lab.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week2</p>
 +
                <p><strong>17/07/2017-23/07/2017</strong></p>
 +
                <p>Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).</p>
 +
                <p>Order Sequence synthesis of ThsS,ThsR,TtrS,TtrR by synbio, Suzhou.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week3</p>
 +
                <p><strong>24/07/2017-30/07/2017</strong></p>
 +
                <p>Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week4</p>
 +
                <p><strong>31/07/2017-06/08/2017</strong></p>
 +
                <p>Order Primers for constructing BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013 parts by Golden Gate.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week5</p>
 +
                <p><strong>07/08/2017-13/08/2017</strong></p>
 +
                <p>Receive synthesized sequences from synbio, and started PCR and then Golden Gate.</p>
 +
                <p>Failed several times of constructing the parts.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week6</p>
 +
                <p><strong>14/08/2017-20/08/2017</strong></p>
 +
                <p>Succeed construct BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013.</p>
 +
                <p>Order primers for constructing BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.</p>
 +
                <p>Get E.coli Nissle 1917 from BNDS_China.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week7</p>
 +
                <p><strong>21/08/2017-27/08/2017</strong></p>
 +
                <p>Construct BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.</p>
 +
                <p>Test which chromoprotein can be obvious in curry (replace excrement) for the first time.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week8</p>
 +
                <p><strong>28/08/2017-03/09/2017</strong></p>
 +
                <p>Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp.</p>
 +
                <p>Qualitative test the response curve of ThsSR-pink, purple, blue/ TtrSR-pink, purple, blue.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week9</p>
 +
                <p><strong>04/09/2017-10/09/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the first time.</p>
 +
                <p>Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp in aerobic and anaerobic condition.</p>
 +
                <p>Go to the clinical laboratory of navy general hospital and made an interview</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week10</p>
 +
                <p><strong>11/09/2017-17/09/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the second time.</p>
 +
                <p>Construct Biobrick BBa_K2507005, BBa_K2507007.</p>
 +
                <p>Construct Biobrick BBa_K2507012, BBa_K25070017.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week11</p>
 +
                <p><strong>18/09/2017-24/09/2017</strong></p>
 +
                <p>Transform Biobrick BBa_K2507004 with BBa_K2507012, and BBa_K2507006 with BBa_K2507017 into E. Coli Top10.</p>
 +
                <p>Test ThsSR-vio/TtrSR-vio system.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week12</p>
 +
                <p><strong>25/09/2017-01/10/2017</strong></p>
 +
                <p>Construct Biobrick BBa_K2507000-004.</p>
 +
                <p>Establish synthetic biology club in SHSBNU.</p>
 +
                <p>Update safety form.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week13</p>
 +
                <p><strong>02/10/2017-08/10/2017</strong></p>
 +
                <p>Start test chemical transform of <i>Bacillus subtilis.</i></p>
 +
                <p>Design Safety devices.
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week14</p>
 +
                <p><strong>09/10/2017-15/10/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the third time.</p>
 +
                <p>Model our project.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week15</p>
 +
                <p><strong>16/10/2017-22/10/2017</strong></p>
 +
                <p>Send part to Genscript.</p>
 
             </div><!-- .entry -->
 
             </div><!-- .entry -->
  

Latest revision as of 19:22, 1 November 2017

SHSBNU-China iGEM 2017 Home Page

Notebook

SHSBNU_China iGEM2017 would like to share with you the document of the work done every week for our project. Although we have only 6 team members in the team, we go all out to do the experiments.

Week1

10/07/2017-16/07/2017

Do Interlab experiment at Peking iGEM team lab.

Week2

17/07/2017-23/07/2017

Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Order Sequence synthesis of ThsS,ThsR,TtrS,TtrR by synbio, Suzhou.

Week3

24/07/2017-30/07/2017

Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Week4

31/07/2017-06/08/2017

Order Primers for constructing BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013 parts by Golden Gate.

Week5

07/08/2017-13/08/2017

Receive synthesized sequences from synbio, and started PCR and then Golden Gate.

Failed several times of constructing the parts.

Week6

14/08/2017-20/08/2017

Succeed construct BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013.

Order primers for constructing BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.

Get E.coli Nissle 1917 from BNDS_China.

Week7

21/08/2017-27/08/2017

Construct BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.

Test which chromoprotein can be obvious in curry (replace excrement) for the first time.

Week8

28/08/2017-03/09/2017

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp.

Qualitative test the response curve of ThsSR-pink, purple, blue/ TtrSR-pink, purple, blue.

Week9

04/09/2017-10/09/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the first time.

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp in aerobic and anaerobic condition.

Go to the clinical laboratory of navy general hospital and made an interview

Week10

11/09/2017-17/09/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the second time.

Construct Biobrick BBa_K2507005, BBa_K2507007.

Construct Biobrick BBa_K2507012, BBa_K25070017.

Week11

18/09/2017-24/09/2017

Transform Biobrick BBa_K2507004 with BBa_K2507012, and BBa_K2507006 with BBa_K2507017 into E. Coli Top10.

Test ThsSR-vio/TtrSR-vio system.

Week12

25/09/2017-01/10/2017

Construct Biobrick BBa_K2507000-004.

Establish synthetic biology club in SHSBNU.

Update safety form.

Week13

02/10/2017-08/10/2017

Start test chemical transform of Bacillus subtilis.

Design Safety devices.

Week14

09/10/2017-15/10/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the third time.

Model our project.

Week15

16/10/2017-22/10/2017

Send part to Genscript.

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division