Difference between revisions of "Team:Uppsala/Safety"

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<h3>Working with microorganisms</h3>
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<p>When working with class 1 bacteria it is important to consider the potential harm it can cause, both towards the students and the environment. That why we had several precautions to make sure the risk was as limited as possible. The lab worked in is classified as biosafety level 1. This allowed us to work with non-pathogenic bacteria such as Escherichia coli DH5α. To limit the spread of bacteria in the lab 70% ethanol was frequently used on benches and other surfaces as well as the lab crews hands to disinfect. This way the bacteria is killed off straight away and its risk of spreading both in the environment and in us is reduced. All liquid bacterial cultures and LB/SOB that may have been contaminated were treated with “Jodopax”, which is an iodine rich substance that kill bacteria. The Jodopax was left in the bacterial solution for at least 1h before disposing of it in the sink. All glass pipettes used were put into buckets with “Virkon” solution to ensure sterilization. They are left in the buckets for at least one day. </p>
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<h3>Hazardous chemicals</h3>
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<p><b>Acrylamide:</b><br>
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Acrylamide is used in SDS-PAGE. The substance is toxic which can cause skin and eye irritation and can cause after long exposure organ damage. To protect us when using the substance thick nitrile gloves, safety glasses and lab coats were used. When handling the substance the one working with it was always extra cautious and disposed anything that might have come in contact with the substance in a special hazardous waste container.
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Revision as of 15:21, 29 October 2017