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Revision as of 18:33, 29 October 2017
RESULTS:
OBJECTIVE: CREATE pgRNA
OBJECTIVE: CREATE REPORTER PLASMID
OBJECTIVE: PROMOTER LIBRARY
OBJECTIVE: RANDOM LIGATIONS
OBJECTIVE: FREEZE DRYING
OBJECTIVE: CRISPRi & guideRNA EFFICIENCY
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.