Difference between revisions of "Team:UNOTT/Results"

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<h4>OBJECTIVE: CREATE pgRNA</h4>
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  <h4>STEP 1: Create guideRNA Plasmid</h4>
  
 
        
 
        
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   <h4>OBJECTIVE: CREATE REPORTER PLASMID</h4>
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   <h4>STEP 2: Create Reporter Plasmid </h4>
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   <h4>OBJECTIVE: PROMOTER LIBRARY</h4>
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   <h4>STEP 3 : Create Promoter Library</h4>
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  <h4>OBJECTIVE: RANDOM LIGATIONS</h4>
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  <h4>STEP 4 : Random Ligations      </h4>
 
        
 
        
 
    
 
    
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  <h4 style="color:#4b524a; font-weight: bold; font-size: 30px;">OBJECTIVE: FREEZE DRYING</h4>
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  <h4>STEP 5: Freeze drying & Revivial</h4>
 
        
 
        
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  <h4>OBJECTIVE: CRISPRi & guideRNA EFFICIENCY</h4>
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  <h4>STEP 6: CRISPRi & guideRNA efficiency</h4>
 
        
 
        
 
<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,
 
<p>This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid,

Revision as of 18:39, 29 October 2017





RESULTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3 : Create Promoter Library

STEP 4 : Random Ligations

STEP 5: Freeze drying & Revivial

STEP 6: CRISPRi & guideRNA efficiency

This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.