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Line 60: | Line 60: | ||
} | } | ||
+ | .wrapper { | ||
+ | width: 50%; | ||
+ | margin: 0 auto; | ||
+ | padding: 3em 0 0; | ||
+ | } | ||
+ | .dropdown { | ||
+ | float: left; | ||
+ | width: 100%; | ||
+ | position: relative; | ||
+ | padding-bottom: 2em; | ||
+ | } | ||
+ | |||
+ | .dropdown input[type=checkbox] { | ||
+ | position: absolute; | ||
+ | top: 0; | ||
+ | left: 0; | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | opacity: 0; | ||
+ | cursor: pointer; | ||
+ | } | ||
+ | |||
+ | .dropdown label { | ||
+ | cursor: pointer; | ||
+ | display: inline-block; | ||
+ | padding-left: 1.75rem; | ||
+ | font-size: 1.4rem; | ||
+ | line-height: 2rem; | ||
+ | font-weight: 600; | ||
+ | color: #333; | ||
+ | font-family: Arial, sans-serif; | ||
+ | } | ||
+ | |||
+ | .dropdown label:before { | ||
+ | content: ""; | ||
+ | position: absolute; | ||
+ | top: 6px; | ||
+ | left: 0; | ||
+ | border-left: 8px solid #F5634A; | ||
+ | border-top: 8px solid transparent; | ||
+ | border-bottom: 8px solid transparent; | ||
+ | margin-left: 4px; | ||
+ | } | ||
+ | |||
+ | .dropdown input[type=checkbox]:checked ~ label:before { | ||
+ | border-left: 8px solid transparent; | ||
+ | border-top: 8px solid #F5634A; | ||
+ | border-right: 8px solid transparent; | ||
+ | margin: 6px 0 0; | ||
+ | } | ||
+ | |||
+ | .dropdown .text { | ||
+ | max-height: 0; | ||
+ | -webkit-transition: max-height 1s; | ||
+ | -moz-transition: max-height 1s; | ||
+ | -ms-transition: max-height 1s; | ||
+ | -o-transition: max-height 1s; | ||
+ | transition: max-height 1s; | ||
+ | overflow: hidden; | ||
+ | font-family: Georgia, serif; | ||
+ | font-size: 1.2rem; | ||
+ | line-height: 1.6rem; | ||
+ | letter-spacing: 0.05em; | ||
+ | color: #333; | ||
+ | padding-left: 1.75rem; | ||
+ | } | ||
+ | |||
+ | .dropdown input[type=checkbox]:checked ~ .text { | ||
+ | max-height: 100px; | ||
+ | } | ||
</style> | </style> | ||
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</div> | </div> | ||
+ | |||
+ | <div class="wrapper"> | ||
+ | <div class="dropdown"> | ||
+ | <input type="checkbox" id="question-1"> | ||
+ | <label for="question-1">Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor?</label> | ||
+ | <div class="text"> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="dropdown"> | ||
+ | <input type="checkbox" id="question-2"> | ||
+ | <label for="question-2">Lorem ipsum dolor sit amet, consectetur adipisicing?</label> | ||
+ | <div class="text"> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="dropdown"> | ||
+ | <input type="checkbox" id="question-3"> | ||
+ | <label for="question-3">Magna aliqua ut enim ad minim?</label> | ||
+ | <div class="text"> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="dropdown"> | ||
+ | <input type="checkbox" id="question-4"> | ||
+ | <label for="question-4">Lorem ipsum dolor sit amet, consectetur adipisicing?</label> | ||
+ | <div class="text"> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
Revision as of 20:00, 29 October 2017
![](https://static.igem.org/mediawiki/2017/0/03/T--UNOTT--Results.jpeg)
RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Create Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.
![](https://static.igem.org/mediawiki/2017/6/6a/T--UNOTT--gRNAresultspic.jpeg)
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Enim ad minim veniam.